Investigating the role of chromatin accessibility in the global transcription regulation in Leishmania quiescent cellusing multiomic single cell approaches

Project Details

Description

Quiescence is a physiological state allowing micro-organisms to survive many environmental insults
and is characterized by a reversible cell division arrest and metabolic downregulation. It is essential
for the perpetuation of pathogen populations, contributes to the success of parasitism and might
explain several (sub-)clinical features like asymptomatic infections, relapses and treatment failure
(without drug resistance). Quiescence was recently demonstrated in Leishmania, but is still understudied.
One of the common features of quiescent cells is chromatin condensation, which is likely a
mechanism to downregulate transcription, protect the genome, and keep the cell at minimal
metabolic activity. It is currently unknown whether changes in chromatin conformation occur in
Leishmania quiescent cells. In this project focusing on Leishmania donovani, we will investigate the
chromatin accessibility during the entry, maintenance and exit of quiescence and correlate it with the
observed transcriptional changes. We will consider two quiescence models based on starvation and
exposure to drugs, respectively. These will be studied at single cell level by applying a cutting edge
method combining an assay for transposase-accessible chromatin using sequencing (ATACseq) and
RNAseq, allowing the simultaneous measurements in the same cells across thousands of cells.
AcronymLEISHCHROMAQ
StatusNot started
Effective start/end date1/12/2130/11/22

Funding

  • DIVERSE KLANTEN: €25,000.00