A dual-chamber model of the female genital tract to evaluate epithelial toxicity of candidate anti-HIV microbicides

KK Ariën; G Vanham; Y Gali

doi:10.1002/0471143030.cb2613s52

A dual-chamber model of the female genital tract to evaluate epithelial toxicity of candidate anti-HIV microbicides

KK Ariën, G Vanham, Y Gali

Research output: Contribution to journal › A2: International peer reviewed article (not A1-type) › peer-review

Abstract

Heterosexual transmission of human immunodeficiency virus (HIV) is the predominant mode of infection worldwide. The early steps of transepithelial infection are crucial, but how exactly infection is established in the female genital tract (FGT) is still under debate. Using epithelial cells originating from the FGT and primary cells as subepithelial HIV target cells, an in vitro dual-chamber model of the FGT was developed. Here we describe how this in vitro model can be used to assess the cellular toxicity and anti-HIV activity of antiretrovirals and formulations thereof that are intended to be used as microbicides.
Curr. Protoc. Cell Biol. 52:26.13.1-26.13.17. (c) 2011 by John Wiley & Sons, Inc.

Original language	English
Journal	Current Protocols in Cell Biology
Volume	52
Pages (from-to)	26.13.1-26.13.17
Number of pages	17
ISSN	1934-2500
DOIs	https://doi.org/10.1002/0471143030.cb2613s52
Publication status	Published - 2011

Keywords

B780-tropical-medicine
Viral diseases
HIV
AIDS
Prevention
Disease transmission-sexual
Microbicides
Epithelial cells
Women
Genital tract
Primary
Dual-chamber model
In vitro
Cellular
Toxicity
Antiretrovirals

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10.1002/0471143030.cb2613s52

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@article{de3877fad971495096df32f59119baaf,

title = "A dual-chamber model of the female genital tract to evaluate epithelial toxicity of candidate anti-HIV microbicides",

abstract = "Heterosexual transmission of human immunodeficiency virus (HIV) is the predominant mode of infection worldwide. The early steps of transepithelial infection are crucial, but how exactly infection is established in the female genital tract (FGT) is still under debate. Using epithelial cells originating from the FGT and primary cells as subepithelial HIV target cells, an in vitro dual-chamber model of the FGT was developed. Here we describe how this in vitro model can be used to assess the cellular toxicity and anti-HIV activity of antiretrovirals and formulations thereof that are intended to be used as microbicides. Curr. Protoc. Cell Biol. 52:26.13.1-26.13.17. (c) 2011 by John Wiley & Sons, Inc.",

keywords = "B780-tropical-medicine, Viral diseases, HIV, AIDS, Prevention, Disease transmission-sexual, Microbicides, Epithelial cells, Women, Genital tract, Primary, Dual-chamber model, In vitro, Cellular, Toxicity, Antiretrovirals",

author = "KK Ari{\"e}n and G Vanham and Y Gali",

note = "ITG-M1A; ITG-M2A; ITG-MLB; MICRO; U-VIROL; E-only; Abstract; Not available",

year = "2011",

doi = "10.1002/0471143030.cb2613s52",

language = "English",

volume = "52",

pages = "26.13.1--26.13.17",

journal = "Current Protocols in Cell Biology",

issn = "1934-2500",

publisher = "John Wiley & Sons",

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TY - JOUR

T1 - A dual-chamber model of the female genital tract to evaluate epithelial toxicity of candidate anti-HIV microbicides

AU - Ariën, KK

AU - Vanham, G

AU - Gali, Y

N1 - ITG-M1A; ITG-M2A; ITG-MLB; MICRO; U-VIROL; E-only; Abstract; Not available

PY - 2011

Y1 - 2011

N2 - Heterosexual transmission of human immunodeficiency virus (HIV) is the predominant mode of infection worldwide. The early steps of transepithelial infection are crucial, but how exactly infection is established in the female genital tract (FGT) is still under debate. Using epithelial cells originating from the FGT and primary cells as subepithelial HIV target cells, an in vitro dual-chamber model of the FGT was developed. Here we describe how this in vitro model can be used to assess the cellular toxicity and anti-HIV activity of antiretrovirals and formulations thereof that are intended to be used as microbicides. Curr. Protoc. Cell Biol. 52:26.13.1-26.13.17. (c) 2011 by John Wiley & Sons, Inc.

AB - Heterosexual transmission of human immunodeficiency virus (HIV) is the predominant mode of infection worldwide. The early steps of transepithelial infection are crucial, but how exactly infection is established in the female genital tract (FGT) is still under debate. Using epithelial cells originating from the FGT and primary cells as subepithelial HIV target cells, an in vitro dual-chamber model of the FGT was developed. Here we describe how this in vitro model can be used to assess the cellular toxicity and anti-HIV activity of antiretrovirals and formulations thereof that are intended to be used as microbicides. Curr. Protoc. Cell Biol. 52:26.13.1-26.13.17. (c) 2011 by John Wiley & Sons, Inc.

KW - B780-tropical-medicine

KW - Viral diseases

KW - HIV

KW - AIDS

KW - Prevention

KW - Disease transmission-sexual

KW - Microbicides

KW - Epithelial cells

KW - Women

KW - Genital tract

KW - Primary

KW - Dual-chamber model

KW - In vitro

KW - Cellular

KW - Toxicity

KW - Antiretrovirals

U2 - 10.1002/0471143030.cb2613s52

DO - 10.1002/0471143030.cb2613s52

M3 - A2: International peer reviewed article (not A1-type)

SN - 1934-2500

VL - 52

SP - 26.13.1-26.13.17

JO - Current Protocols in Cell Biology

JF - Current Protocols in Cell Biology

ER -

A dual-chamber model of the female genital tract to evaluate epithelial toxicity of candidate anti-HIV microbicides

Abstract

Keywords

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