A dual-chamber model of the female genital tract to evaluate epithelial toxicity of candidate anti-HIV microbicides

KK Ariën, G Vanham, Y Gali

    Research output: Contribution to journalA2: International peer reviewed article (not A1-type)

    Abstract

    Heterosexual transmission of human immunodeficiency virus (HIV) is the predominant mode of infection worldwide. The early steps of transepithelial infection are crucial, but how exactly infection is established in the female genital tract (FGT) is still under debate. Using epithelial cells originating from the FGT and primary cells as subepithelial HIV target cells, an in vitro dual-chamber model of the FGT was developed. Here we describe how this in vitro model can be used to assess the cellular toxicity and anti-HIV activity of antiretrovirals and formulations thereof that are intended to be used as microbicides.
    Curr. Protoc. Cell Biol. 52:26.13.1-26.13.17. (c) 2011 by John Wiley & Sons, Inc.
    Original languageEnglish
    JournalCurrent Protocols in Cell Biology
    Volume52
    Pages (from-to)26.13.1-26.13.17
    Number of pages17
    ISSN1934-2616
    DOIs
    Publication statusPublished - 2011

    Keywords

    • B780-tropical-medicine
    • Viral diseases
    • HIV
    • AIDS
    • Prevention
    • Disease transmission-sexual
    • Microbicides
    • Epithelial cells
    • Women
    • Genital tract
    • Primary
    • Dual-chamber model
    • In vitro
    • Cellular
    • Toxicity
    • Antiretrovirals

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