A LiTat 1.5 variant surface glycoprotein-derived peptide with diagnostic potential for Trypanosoma brucei gambiense

L. Van Nieuwenhove, Philippe Büscher, F. Balharbi, M. Humbert, Y. Guisez, V. Lejon

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    OBJECTIVE: To evaluate the accuracy of a peptide, corresponding to the variant surface glycoprotein (VSG) LiTat 1.5 amino acid (AA) sequence 268-281 and identified through alignment of monoclonal antibody selected mimotopes, for diagnosis of Trypanosoma brucei gambiense sleeping sickness. METHODS: A synthetic biotinylated peptide (peptide 1.5/268-281), native VSG LiTat 1.3 and VSG LiTat 1.5 were tested in an indirect ELISA with 102 sera from patients with HAT and 102 endemic HAT-negative controls. RESULTS: The area under the curve (AUC) of peptide 1.5/268-281 was 0.954 (95% confidence interval 0.918-0.980), indicating diagnostic potential. The areas under the curve of VSG LiTat 1.3 and LiTat 1.5 were 1.000 (0.982-1.000) and 0.997 (0.973-1.000), respectively, and significantly higher than the AUC of peptide 1.5/268-281. On a model of VSG LiTat 1.5, peptide 1.5/268-281 was mapped near the top of the VSG. CONCLUSIONS: A biotinylated peptide corresponding to AA 268-281 of VSG LiTat 1.5 may replace the native VSG in serodiagnostic tests, but the diagnostic accuracy is lower than for the full-length native VSG LiTat 1.3 and VSG LiTat 1.5.
    Original languageEnglish
    JournalTropical Medicine and International Health
    Volume18
    Issue number4
    Pages (from-to)461-465
    Number of pages5
    ISSN1360-2276
    DOIs
    Publication statusPublished - 2013

    Keywords

    • Protozoal diseases
    • Sleeping sickness
    • Trypanosomiasis-African
    • Trypanosoma brucei gambiense
    • Vectors
    • Tsetse flies
    • Glossina morsitans
    • Diagnosis
    • Peptides
    • Variable surface glycoproteins
    • VSG
    • LiTat 1.5
    • Amino acid sequences
    • Mimotopes
    • Accuracy
    • Specificity
    • Sensitivity
    • Laboratory techniques and procedures

    Fingerprint

    Dive into the research topics of 'A LiTat 1.5 variant surface glycoprotein-derived peptide with diagnostic potential for <i>Trypanosoma brucei gambiense</i>'. Together they form a unique fingerprint.

    Cite this