Abstract
OBJECTIVE: To evaluate the accuracy of a peptide, corresponding to the variant surface glycoprotein (VSG) LiTat 1.5 amino acid (AA) sequence 268-281 and identified through alignment of monoclonal antibody selected mimotopes, for diagnosis of Trypanosoma brucei gambiense sleeping sickness. METHODS: A synthetic biotinylated peptide (peptide 1.5/268-281), native VSG LiTat 1.3 and VSG LiTat 1.5 were tested in an indirect ELISA with 102 sera from patients with HAT and 102 endemic HAT-negative controls. RESULTS: The area under the curve (AUC) of peptide 1.5/268-281 was 0.954 (95% confidence interval 0.918-0.980), indicating diagnostic potential. The areas under the curve of VSG LiTat 1.3 and LiTat 1.5 were 1.000 (0.982-1.000) and 0.997 (0.973-1.000), respectively, and significantly higher than the AUC of peptide 1.5/268-281. On a model of VSG LiTat 1.5, peptide 1.5/268-281 was mapped near the top of the VSG. CONCLUSIONS: A biotinylated peptide corresponding to AA 268-281 of VSG LiTat 1.5 may replace the native VSG in serodiagnostic tests, but the diagnostic accuracy is lower than for the full-length native VSG LiTat 1.3 and VSG LiTat 1.5.
Original language | English |
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Journal | Tropical Medicine and International Health |
Volume | 18 |
Issue number | 4 |
Pages (from-to) | 461-465 |
Number of pages | 5 |
ISSN | 1360-2276 |
DOIs | |
Publication status | Published - 2013 |
Keywords
- Protozoal diseases
- Sleeping sickness
- Trypanosomiasis-African
- Trypanosoma brucei gambiense
- Vectors
- Tsetse flies
- Glossina morsitans
- Diagnosis
- Peptides
- Variable surface glycoproteins
- VSG
- LiTat 1.5
- Amino acid sequences
- Mimotopes
- Accuracy
- Specificity
- Sensitivity
- Laboratory techniques and procedures