A LiTat 1.5 variant surface glycoprotein-derived peptide with diagnostic potential for Trypanosoma brucei gambiense

L. Van Nieuwenhove, Philippe Büscher, F. Balharbi, M. Humbert, Y. Guisez, V. Lejon

Research output: Contribution to journalA1: Web of Science-article

Abstract

OBJECTIVE: To evaluate the accuracy of a peptide, corresponding to the variant surface glycoprotein (VSG) LiTat 1.5 amino acid (AA) sequence 268-281 and identified through alignment of monoclonal antibody selected mimotopes, for diagnosis of Trypanosoma brucei gambiense sleeping sickness. METHODS: A synthetic biotinylated peptide (peptide 1.5/268-281), native VSG LiTat 1.3 and VSG LiTat 1.5 were tested in an indirect ELISA with 102 sera from patients with HAT and 102 endemic HAT-negative controls. RESULTS: The area under the curve (AUC) of peptide 1.5/268-281 was 0.954 (95% confidence interval 0.918-0.980), indicating diagnostic potential. The areas under the curve of VSG LiTat 1.3 and LiTat 1.5 were 1.000 (0.982-1.000) and 0.997 (0.973-1.000), respectively, and significantly higher than the AUC of peptide 1.5/268-281. On a model of VSG LiTat 1.5, peptide 1.5/268-281 was mapped near the top of the VSG. CONCLUSIONS: A biotinylated peptide corresponding to AA 268-281 of VSG LiTat 1.5 may replace the native VSG in serodiagnostic tests, but the diagnostic accuracy is lower than for the full-length native VSG LiTat 1.3 and VSG LiTat 1.5.
Original languageEnglish
JournalTropical Medicine and International Health
Volume18
Issue number4
Pages (from-to)461-465
Number of pages5
ISSN1360-2276
DOIs
Publication statusPublished - 2013

Keywords

  • Protozoal diseases
  • Sleeping sickness
  • Trypanosomiasis-African
  • Trypanosoma brucei gambiense
  • Vectors
  • Tsetse flies
  • Glossina morsitans
  • Diagnosis
  • Peptides
  • Variable surface glycoproteins
  • VSG
  • LiTat 1.5
  • Amino acid sequences
  • Mimotopes
  • Accuracy
  • Specificity
  • Sensitivity
  • Laboratory techniques and procedures

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