A modified semi-nested multiplex malaria PCR (SnM-PCR) for the identification of the five human Plasmodium species occurring in Southeast Asia

N. Hong, P. Van den Eede, C. Van Overmeir, I. Vythilingham, A. Rosanas-Urgell, P. Vinh Thanh, N. Thang, N. Hung, L. Hung, Umberto D'Alessandro, A. Erhart

Research output: Contribution to journalA1: Web of Science-article

Abstract

We have modified an existing semi-nested multiplex polymerase chain reaction (PCR) by adding one Plasmodium knowlesi-specific nested PCR, and validated the latter against laboratory and clinical samples. This new method has the advantage of being relatively affordable in low resource settings while identifying the five human Plasmodium species with a three-step PCR.
Original languageEnglish
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume89
Issue number4
Pages (from-to)721-733
Number of pages13
ISSN0002-9637
DOIs
Publication statusPublished - 2013

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