A modified semi-nested multiplex malaria PCR (SnM-PCR) for the identification of the five human Plasmodium species occurring in Southeast Asia

N. Hong; P. Van den Eede; C. Van Overmeir; I. Vythilingham; A. Rosanas-Urgell; P. Vinh Thanh; N. Thang; N. Hung; L. Hung; Umberto D'Alessandro; A. Erhart

doi:10.4269/ajtmh.13-0027

A modified semi-nested multiplex malaria PCR (SnM-PCR) for the identification of the five human Plasmodium species occurring in Southeast Asia

N. Hong, P. Van den Eede, C. Van Overmeir, I. Vythilingham, A. Rosanas-Urgell, P. Vinh Thanh, N. Thang, N. Hung, L. Hung, Umberto D'Alessandro, A. Erhart

Malariology

Research output: Contribution to journal › A1: Web of Science-article › peer-review

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Abstract

We have modified an existing semi-nested multiplex polymerase chain reaction (PCR) by adding one Plasmodium knowlesi-specific nested PCR, and validated the latter against laboratory and clinical samples. This new method has the advantage of being relatively affordable in low resource settings while identifying the five human Plasmodium species with a three-step PCR.

Original language	English
Journal	American Journal of Tropical Medicine and Hygiene
Volume	89
Issue number	4
Pages (from-to)	721-733
Number of pages	13
ISSN	0002-9637
DOIs	https://doi.org/10.4269/ajtmh.13-0027
Publication status	Published - 2013

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10.4269/ajtmh.13-0027Licence: CC BY

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Hong, N., Van den Eede, P., Van Overmeir, C., Vythilingham, I., Rosanas-Urgell, A., Vinh Thanh, P., Thang, N., Hung, N., Hung, L., D'Alessandro, U., & Erhart, A. (2013). A modified semi-nested multiplex malaria PCR (SnM-PCR) for the identification of the five human Plasmodium species occurring in Southeast Asia. American Journal of Tropical Medicine and Hygiene, 89(4), 721-733. https://doi.org/10.4269/ajtmh.13-0027

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abstract = "We have modified an existing semi-nested multiplex polymerase chain reaction (PCR) by adding one Plasmodium knowlesi-specific nested PCR, and validated the latter against laboratory and clinical samples. This new method has the advantage of being relatively affordable in low resource settings while identifying the five human Plasmodium species with a three-step PCR.",

author = "N. Hong and {Van den Eede}, P. and {Van Overmeir}, C. and I. Vythilingham and A. Rosanas-Urgell and {Vinh Thanh}, P. and N. Thang and N. Hung and L. Hung and Umberto D'Alessandro and A. Erhart",

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language = "English",

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Hong, N, Van den Eede, P, Van Overmeir, C, Vythilingham, I, Rosanas-Urgell, A, Vinh Thanh, P, Thang, N, Hung, N, Hung, L, D'Alessandro, U & Erhart, A 2013, 'A modified semi-nested multiplex malaria PCR (SnM-PCR) for the identification of the five human Plasmodium species occurring in Southeast Asia', American Journal of Tropical Medicine and Hygiene, vol. 89, no. 4, pp. 721-733. https://doi.org/10.4269/ajtmh.13-0027

A modified semi-nested multiplex malaria PCR (SnM-PCR) for the identification of the five human Plasmodium species occurring in Southeast Asia. / Hong, N.; Van den Eede, P.; Van Overmeir, C. et al.
In: American Journal of Tropical Medicine and Hygiene, Vol. 89, No. 4, 2013, p. 721-733.

Research output: Contribution to journal › A1: Web of Science-article › peer-review

TY - JOUR

T1 - A modified semi-nested multiplex malaria PCR (SnM-PCR) for the identification of the five human Plasmodium species occurring in Southeast Asia

AU - Hong, N.

AU - Van den Eede, P.

AU - Van Overmeir, C.

AU - Vythilingham, I.

AU - Rosanas-Urgell, A.

AU - Vinh Thanh, P.

AU - Thang, N.

AU - Hung, N.

AU - Hung, L.

AU - D'Alessandro, Umberto

AU - Erhart, A.

N1 - FTX; CC BY

PY - 2013

Y1 - 2013

N2 - We have modified an existing semi-nested multiplex polymerase chain reaction (PCR) by adding one Plasmodium knowlesi-specific nested PCR, and validated the latter against laboratory and clinical samples. This new method has the advantage of being relatively affordable in low resource settings while identifying the five human Plasmodium species with a three-step PCR.

AB - We have modified an existing semi-nested multiplex polymerase chain reaction (PCR) by adding one Plasmodium knowlesi-specific nested PCR, and validated the latter against laboratory and clinical samples. This new method has the advantage of being relatively affordable in low resource settings while identifying the five human Plasmodium species with a three-step PCR.

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000326122100017

U2 - 10.4269/ajtmh.13-0027

DO - 10.4269/ajtmh.13-0027

M3 - A1: Web of Science-article

C2 - 23980132

SN - 0002-9637

VL - 89

SP - 721

EP - 733

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

IS - 4

ER -

A modified semi-nested multiplex malaria PCR (SnM-PCR) for the identification of the five human Plasmodium species occurring in Southeast Asia

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