A new PCR-ELISA for diagnosis of visceral leishmaniasis in blood of HIV-negative subjects

S De Doncker; V Hutse; S Abdellati; S Rijal; BMS Karki; S Decuypere; D Jacquet; D Le Ray; M Boelaert; S Koirala; JC Dujardin

doi:10.1016/j.trstmh.2004.01.015

A new PCR-ELISA for diagnosis of visceral leishmaniasis in blood of HIV-negative subjects

S De Doncker, V Hutse, S Abdellati, S Rijal, BMS Karki, S Decuypere, D Jacquet, D Le Ray, M Boelaert, S Koirala, JC Dujardin

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

The PCR-ELISA represents a promising advance for diagnosis of visceral. leishmaniasis (VL) in blood samples. However, the method has been validated mostly with HIV-positive patients who are known to have high levels of parasitaemia. We developed a new PCR-ELISA assay for specific detection of Leishmania in patients' blood and validated it in Nepalese subjects with clinically suspected VL, almost all of whom were HIV-negative. For blood samples, PCR-ELISA was more sensitive (83.9%) than conventional PCR (73.2%), and demonstrated 100% and 87.2% specificity when using healthy controls who had never travelled to a VL-endemic area and controls from a VL-endemic area as references, respectively. We have demonstrated the ability of PCR-ELISA to detect parasites in blood of HIV-negative patients. The method could be used for epidemiological as well as clinical purposes, as it reduces the need for traumatic bone marrow sampling and risky spleen aspiration.

Original language	English
Journal	Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume	99
Issue number	1
Pages (from-to)	25-31
ISSN	0035-9203
DOIs	https://doi.org/10.1016/j.trstmh.2004.01.015
Publication status	Published - 2005

Keywords

B780-tropical-medicine
Protozoal diseases
Leishmaniasis
Visceral
Molecular diagnostic techniques
Blood
PCR-ELISA
Sensitivity
Specificity
Nepal
Asia-South

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10.1016/j.trstmh.2004.01.015

http://lib.itg.be/pdf/itg/2005/2005trst0025.pdf

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De Doncker, S., Hutse, V., Abdellati, S., Rijal, S., Karki, BMS., Decuypere, S., Jacquet, D., Le Ray, D., Boelaert, M., Koirala, S., & Dujardin, JC. (2005). A new PCR-ELISA for diagnosis of visceral leishmaniasis in blood of HIV-negative subjects. Transactions of the Royal Society of Tropical Medicine and Hygiene, 99(1), 25-31. https://doi.org/10.1016/j.trstmh.2004.01.015

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title = "A new PCR-ELISA for diagnosis of visceral leishmaniasis in blood of HIV-negative subjects",

abstract = "The PCR-ELISA represents a promising advance for diagnosis of visceral. leishmaniasis (VL) in blood samples. However, the method has been validated mostly with HIV-positive patients who are known to have high levels of parasitaemia. We developed a new PCR-ELISA assay for specific detection of Leishmania in patients' blood and validated it in Nepalese subjects with clinically suspected VL, almost all of whom were HIV-negative. For blood samples, PCR-ELISA was more sensitive (83.9%) than conventional PCR (73.2%), and demonstrated 100% and 87.2% specificity when using healthy controls who had never travelled to a VL-endemic area and controls from a VL-endemic area as references, respectively. We have demonstrated the ability of PCR-ELISA to detect parasites in blood of HIV-negative patients. The method could be used for epidemiological as well as clinical purposes, as it reduces the need for traumatic bone marrow sampling and risky spleen aspiration.",

keywords = "B780-tropical-medicine, Protozoal diseases, Leishmaniasis, Visceral, Molecular diagnostic techniques, Blood, PCR-ELISA, Sensitivity, Specificity, Nepal, Asia-South",

author = "{De Doncker}, S and V Hutse and S Abdellati and S Rijal and BMS Karki and S Decuypere and D Jacquet and {Le Ray}, D and M Boelaert and S Koirala and JC Dujardin",

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De Doncker, S, Hutse, V, Abdellati, S, Rijal, S, Karki, BMS, Decuypere, S, Jacquet, D, Le Ray, D, Boelaert, M, Koirala, S & Dujardin, JC 2005, 'A new PCR-ELISA for diagnosis of visceral leishmaniasis in blood of HIV-negative subjects', Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 99, no. 1, pp. 25-31. https://doi.org/10.1016/j.trstmh.2004.01.015

TY - JOUR

T1 - A new PCR-ELISA for diagnosis of visceral leishmaniasis in blood of HIV-negative subjects

AU - De Doncker, S

AU - Hutse, V

AU - Abdellati, S

AU - Rijal, S

AU - Karki, BMS

AU - Decuypere, S

AU - Jacquet, D

AU - Le Ray, D

AU - Boelaert, M

AU - Koirala, S

AU - Dujardin, JC

N1 - FTX abonnement

PY - 2005

Y1 - 2005

N2 - The PCR-ELISA represents a promising advance for diagnosis of visceral. leishmaniasis (VL) in blood samples. However, the method has been validated mostly with HIV-positive patients who are known to have high levels of parasitaemia. We developed a new PCR-ELISA assay for specific detection of Leishmania in patients' blood and validated it in Nepalese subjects with clinically suspected VL, almost all of whom were HIV-negative. For blood samples, PCR-ELISA was more sensitive (83.9%) than conventional PCR (73.2%), and demonstrated 100% and 87.2% specificity when using healthy controls who had never travelled to a VL-endemic area and controls from a VL-endemic area as references, respectively. We have demonstrated the ability of PCR-ELISA to detect parasites in blood of HIV-negative patients. The method could be used for epidemiological as well as clinical purposes, as it reduces the need for traumatic bone marrow sampling and risky spleen aspiration.

AB - The PCR-ELISA represents a promising advance for diagnosis of visceral. leishmaniasis (VL) in blood samples. However, the method has been validated mostly with HIV-positive patients who are known to have high levels of parasitaemia. We developed a new PCR-ELISA assay for specific detection of Leishmania in patients' blood and validated it in Nepalese subjects with clinically suspected VL, almost all of whom were HIV-negative. For blood samples, PCR-ELISA was more sensitive (83.9%) than conventional PCR (73.2%), and demonstrated 100% and 87.2% specificity when using healthy controls who had never travelled to a VL-endemic area and controls from a VL-endemic area as references, respectively. We have demonstrated the ability of PCR-ELISA to detect parasites in blood of HIV-negative patients. The method could be used for epidemiological as well as clinical purposes, as it reduces the need for traumatic bone marrow sampling and risky spleen aspiration.

KW - B780-tropical-medicine

KW - Protozoal diseases

KW - Leishmaniasis

KW - Visceral

KW - Molecular diagnostic techniques

KW - Blood

KW - PCR-ELISA

KW - Sensitivity

KW - Specificity

KW - Nepal

KW - Asia-South

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000225615000006

U2 - 10.1016/j.trstmh.2004.01.015

DO - 10.1016/j.trstmh.2004.01.015

M3 - A1: Web of Science-article

SN - 0035-9203

VL - 99

SP - 25

EP - 31

JO - Transactions of the Royal Society of Tropical Medicine and Hygiene

JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

IS - 1

ER -

A new PCR-ELISA for diagnosis of visceral leishmaniasis in blood of HIV-negative subjects

Abstract

Keywords

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