A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax

B Russell, R Suwanarusk, C Borlon, FTM Costa, CS Chu, MJ Rijken, K Sriprawat, L Warter, EGL Koh, B Malleret, Y Colin, O Bertrand, JH Adams, U D'Alessandro, G Snounou, F Nosten, L Rénia

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    Currently, there are no reliable red blood cells invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P. vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin treated P. vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P. vivax isolates freshly collected from patients in Thailand.
    Original languageEnglish
    JournalBlood
    Volume118
    Issue number13
    Pages (from-to)e74-e81
    Number of pages8
    ISSN0006-4971
    DOIs
    Publication statusPublished - 2011

    Keywords

    • B780-tropical-medicine
    • Protozoal diseases
    • Malaria
    • Plasmodium vivax
    • Protocols
    • Assays
    • Ex vivo
    • Routine
    • Red blood cells
    • Laboratory diagnosis
    • Reticulocytes
    • Schizonts
    • Performance
    • Efficiency
    • Rapid diagnostic tests
    • Reproducibility
    • Laboratory techniques and procedures

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