A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax

B Russell; R Suwanarusk; C Borlon; FTM Costa; CS Chu; MJ Rijken; K Sriprawat; L Warter; EGL Koh; B Malleret; Y Colin; O Bertrand; JH Adams; U D'Alessandro; G Snounou; F Nosten; L Rénia

doi:10.1182/blood-2011-04-348748

A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax

B Russell, R Suwanarusk, C Borlon, FTM Costa, CS Chu, MJ Rijken, K Sriprawat, L Warter, EGL Koh, B Malleret, Y Colin, O Bertrand, JH Adams, U D'Alessandro, G Snounou, F Nosten, L Rénia

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Currently, there are no reliable red blood cells invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P. vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin treated P. vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P. vivax isolates freshly collected from patients in Thailand.

Original language	English
Journal	Blood
Volume	118
Issue number	13
Pages (from-to)	e74-e81
Number of pages	8
ISSN	0006-4971
DOIs	https://doi.org/10.1182/blood-2011-04-348748
Publication status	Published - 2011

Keywords

B780-tropical-medicine
Protozoal diseases
Malaria
Plasmodium vivax
Protocols
Assays
Ex vivo
Routine
Red blood cells
Laboratory diagnosis
Reticulocytes
Schizonts
Performance
Efficiency
Rapid diagnostic tests
Reproducibility
Laboratory techniques and procedures

Access to Document

10.1182/blood-2011-04-348748

http://lib.itg.be/pdf/itg/2011/2011bloo0e74.pdf

Cite this

Russell, B., Suwanarusk, R., Borlon, C., Costa, FTM., Chu, CS., Rijken, MJ., Sriprawat, K., Warter, L., Koh, EGL., Malleret, B., Colin, Y., Bertrand, O., Adams, JH., D'Alessandro, U., Snounou, G., Nosten, F., & Rénia, L. (2011). A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax. Blood, 118(13), e74-e81. https://doi.org/10.1182/blood-2011-04-348748

@article{596b74207f2949a48b7cad66c404f82a,

title = "A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax",

abstract = "Currently, there are no reliable red blood cells invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P. vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin treated P. vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P. vivax isolates freshly collected from patients in Thailand.",

keywords = "B780-tropical-medicine, Protozoal diseases, Malaria, Plasmodium vivax, Protocols, Assays, Ex vivo, Routine, Red blood cells, Laboratory diagnosis, Reticulocytes, Schizonts, Performance, Efficiency, Rapid diagnostic tests, Reproducibility, Laboratory techniques and procedures",

author = "B Russell and R Suwanarusk and C Borlon and FTM Costa and CS Chu and MJ Rijken and K Sriprawat and L Warter and EGL Koh and B Malleret and Y Colin and O Bertrand and JH Adams and U D'Alessandro and G Snounou and F Nosten and L R{\'e}nia",

note = "NPP; ITG-P3B; ITG-P14A; PARAS; U-MALAR; JIF; E-only; DOI; PDF; Abstract; DSPACE",

year = "2011",

doi = "10.1182/blood-2011-04-348748",

language = "English",

volume = "118",

pages = "e74--e81",

journal = "Blood",

issn = "0006-4971",

publisher = "American Society of Hematology",

number = "13",

}

Russell, B, Suwanarusk, R, Borlon, C, Costa, FTM, Chu, CS, Rijken, MJ, Sriprawat, K, Warter, L, Koh, EGL, Malleret, B, Colin, Y, Bertrand, O, Adams, JH, D'Alessandro, U, Snounou, G, Nosten, F & Rénia, L 2011, 'A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax', Blood, vol. 118, no. 13, pp. e74-e81. https://doi.org/10.1182/blood-2011-04-348748

TY - JOUR

T1 - A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax

AU - Russell, B

AU - Suwanarusk, R

AU - Borlon, C

AU - Costa, FTM

AU - Chu, CS

AU - Rijken, MJ

AU - Sriprawat, K

AU - Warter, L

AU - Koh, EGL

AU - Malleret, B

AU - Colin, Y

AU - Bertrand, O

AU - Adams, JH

AU - D'Alessandro, U

AU - Snounou, G

AU - Nosten, F

AU - Rénia, L

N1 - NPP; ITG-P3B; ITG-P14A; PARAS; U-MALAR; JIF; E-only; DOI; PDF; Abstract; DSPACE

PY - 2011

Y1 - 2011

N2 - Currently, there are no reliable red blood cells invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P. vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin treated P. vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P. vivax isolates freshly collected from patients in Thailand.

AB - Currently, there are no reliable red blood cells invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P. vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin treated P. vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P. vivax isolates freshly collected from patients in Thailand.

KW - B780-tropical-medicine

KW - Protozoal diseases

KW - Malaria

KW - Plasmodium vivax

KW - Protocols

KW - Assays

KW - Ex vivo

KW - Routine

KW - Red blood cells

KW - Laboratory diagnosis

KW - Reticulocytes

KW - Schizonts

KW - Performance

KW - Efficiency

KW - Rapid diagnostic tests

KW - Reproducibility

KW - Laboratory techniques and procedures

U2 - 10.1182/blood-2011-04-348748

DO - 10.1182/blood-2011-04-348748

M3 - A1: Web of Science-article

C2 - 21768300

SN - 0006-4971

VL - 118

SP - e74-e81

JO - Blood

JF - Blood

IS - 13

ER -

A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax

Abstract

Keywords

Access to Document

Fingerprint

Cite this