A semi-quantitative ELISA for detection of Trypanosoma brucei gambiense specific antibodies in serum and cerebrospinal fluid of sleeping sickness patients

V Lejon; P Büscher; E Magnus; A Moons; I Wouters; N Van Meirvenne

doi:10.1016/S0001-706X(97)00137-X

A semi-quantitative ELISA for detection of Trypanosoma brucei gambiense specific antibodies in serum and cerebrospinal fluid of sleeping sickness patients

V Lejon, P Büscher, E Magnus, A Moons, I Wouters, N Van Meirvenne

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

A semi-quantitative ELISA, using variable surface glycoprotein of T.b. gambiense as antigen, was developed for the detection of antibodies of different immunoglobulin isotypes in serum and cerebrospinal fluid of sleeping sickness patients. Using the assay, the antibody profiles of paired serum and cerebrospinal fluid samples of 28 patients have been studied. Total concentrations of various Ig isotypes were determined as well. In serum and cerebrospinal fluid a drastic increase in IgG, basically IgG1, as a ell as in IgM levels was observed. The concentration of IgA remained relatively normal. The antitrypanosomal antibodies detected in serum and cerebrospinal fluid were mainly of the IgG (IgG1 and IgG3) and IgM isotypes. Measurement of immunoglobulin and trypanosome specific antibody concentrations in serum and CSF allows calculation of intrathecal antibody synthesis and is a possible tool for determining the clinical stage of sleeping sickness.

Original language	English
Journal	Acta Tropica
Volume	69
Issue number	2
Pages (from-to)	151-164
ISSN	0001-706X
DOIs	https://doi.org/10.1016/S0001-706X(97)00137-X
Publication status	Published - 1998

Keywords

B780-tropical-medicine
Protozoal diseases
Trypanosomiasis
Trypanosoma brucei gambiense
Diagnosis
Stage determination
ELISA
Antibodies
Albumin
Serum
Cerebrospinal fluid
CSF

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10.1016/S0001-706X(97)00137-X

http://lib.itg.be/pdf/itg/1998/1998atro0151.pdf

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title = "A semi-quantitative ELISA for detection of Trypanosoma brucei gambiense specific antibodies in serum and cerebrospinal fluid of sleeping sickness patients",

abstract = "A semi-quantitative ELISA, using variable surface glycoprotein of T.b. gambiense as antigen, was developed for the detection of antibodies of different immunoglobulin isotypes in serum and cerebrospinal fluid of sleeping sickness patients. Using the assay, the antibody profiles of paired serum and cerebrospinal fluid samples of 28 patients have been studied. Total concentrations of various Ig isotypes were determined as well. In serum and cerebrospinal fluid a drastic increase in IgG, basically IgG1, as a ell as in IgM levels was observed. The concentration of IgA remained relatively normal. The antitrypanosomal antibodies detected in serum and cerebrospinal fluid were mainly of the IgG (IgG1 and IgG3) and IgM isotypes. Measurement of immunoglobulin and trypanosome specific antibody concentrations in serum and CSF allows calculation of intrathecal antibody synthesis and is a possible tool for determining the clinical stage of sleeping sickness. ",

keywords = "B780-tropical-medicine, Protozoal diseases, Trypanosomiasis, Trypanosoma brucei gambiense, Diagnosis, Stage determination, ELISA, Antibodies, Albumin, Serum, Cerebrospinal fluid, CSF",

author = "V Lejon and P B{\"u}scher and E Magnus and A Moons and I Wouters and {Van Meirvenne}, N",

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T1 - A semi-quantitative ELISA for detection of Trypanosoma brucei gambiense specific antibodies in serum and cerebrospinal fluid of sleeping sickness patients

AU - Lejon, V

AU - Büscher, P

AU - Magnus, E

AU - Moons, A

AU - Wouters, I

AU - Van Meirvenne, N

N1 - FTX abonnement

PY - 1998

Y1 - 1998

N2 - A semi-quantitative ELISA, using variable surface glycoprotein of T.b. gambiense as antigen, was developed for the detection of antibodies of different immunoglobulin isotypes in serum and cerebrospinal fluid of sleeping sickness patients. Using the assay, the antibody profiles of paired serum and cerebrospinal fluid samples of 28 patients have been studied. Total concentrations of various Ig isotypes were determined as well. In serum and cerebrospinal fluid a drastic increase in IgG, basically IgG1, as a ell as in IgM levels was observed. The concentration of IgA remained relatively normal. The antitrypanosomal antibodies detected in serum and cerebrospinal fluid were mainly of the IgG (IgG1 and IgG3) and IgM isotypes. Measurement of immunoglobulin and trypanosome specific antibody concentrations in serum and CSF allows calculation of intrathecal antibody synthesis and is a possible tool for determining the clinical stage of sleeping sickness.

AB - A semi-quantitative ELISA, using variable surface glycoprotein of T.b. gambiense as antigen, was developed for the detection of antibodies of different immunoglobulin isotypes in serum and cerebrospinal fluid of sleeping sickness patients. Using the assay, the antibody profiles of paired serum and cerebrospinal fluid samples of 28 patients have been studied. Total concentrations of various Ig isotypes were determined as well. In serum and cerebrospinal fluid a drastic increase in IgG, basically IgG1, as a ell as in IgM levels was observed. The concentration of IgA remained relatively normal. The antitrypanosomal antibodies detected in serum and cerebrospinal fluid were mainly of the IgG (IgG1 and IgG3) and IgM isotypes. Measurement of immunoglobulin and trypanosome specific antibody concentrations in serum and CSF allows calculation of intrathecal antibody synthesis and is a possible tool for determining the clinical stage of sleeping sickness.

KW - B780-tropical-medicine

KW - Protozoal diseases

KW - Trypanosomiasis

KW - Trypanosoma brucei gambiense

KW - Diagnosis

KW - Stage determination

KW - ELISA

KW - Antibodies

KW - Albumin

KW - Serum

KW - Cerebrospinal fluid

KW - CSF

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000073188300007

U2 - 10.1016/S0001-706X(97)00137-X

DO - 10.1016/S0001-706X(97)00137-X

M3 - A1: Web of Science-article

SN - 0001-706X

VL - 69

SP - 151

EP - 164

JO - Acta Tropica

JF - Acta Tropica

IS - 2

ER -

A semi-quantitative ELISA for detection of Trypanosoma brucei gambiense specific antibodies in serum and cerebrospinal fluid of sleeping sickness patients

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Keywords

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