A simple and economical strategy for downstream processing of specific antibodies to human transferrin from egg yolk

V Ntakarutimana; P Demedts; M van Sande; S Scharpé

doi:10.1016/0022-1759(92)90315-K

A simple and economical strategy for downstream processing of specific antibodies to human transferrin from egg yolk

V Ntakarutimana, P Demedts, M van Sande, S Scharpé

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

A rapid and economical procedure for extraction of antibodies from egg yolk is described. Laying hens were immunized with human transferrin and extracts of egg-yolk were purified with a procedure based on affinity chromatography.

The resulting purified antibodies were evaluated in a nephelometric system for the assay of transferrin in human sera. The results agreed closely with those obtained with a commercially available anti-human transferrin serum from rabbits.

Original language	English
Journal	Journal of Immunological Methods
Volume	153
Issue number	1-2
Pages (from-to)	133-140
Number of pages	8
ISSN	0022-1759
DOIs	https://doi.org/10.1016/0022-1759(92)90315-K
Publication status	Published - 1992

Keywords

B780-tropical-medicine
Immunology
Laboratory
Antibodies
Purification
Chromatography
Transferrin
Nephelometry
Egg-yolk
IgY

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10.1016/0022-1759(92)90315-K

http://lib.itg.be/pdf/itg/1992/1992jime0133.pdf

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title = "A simple and economical strategy for downstream processing of specific antibodies to human transferrin from egg yolk",

abstract = "A rapid and economical procedure for extraction of antibodies from egg yolk is described. Laying hens were immunized with human transferrin and extracts of egg-yolk were purified with a procedure based on affinity chromatography.The resulting purified antibodies were evaluated in a nephelometric system for the assay of transferrin in human sera. The results agreed closely with those obtained with a commercially available anti-human transferrin serum from rabbits.",

keywords = "B780-tropical-medicine, Immunology, Laboratory, Antibodies, Purification, Chromatography, Transferrin, Nephelometry, Egg-yolk, IgY",

author = "V Ntakarutimana and P Demedts and {van Sande}, M and S Scharp{\'e}",

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year = "1992",

doi = "10.1016/0022-1759(92)90315-K",

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TY - JOUR

T1 - A simple and economical strategy for downstream processing of specific antibodies to human transferrin from egg yolk

AU - Ntakarutimana, V

AU - Demedts, P

AU - van Sande, M

AU - Scharpé, S

N1 - FTX: available in ITM print journal collection

PY - 1992

Y1 - 1992

N2 - A rapid and economical procedure for extraction of antibodies from egg yolk is described. Laying hens were immunized with human transferrin and extracts of egg-yolk were purified with a procedure based on affinity chromatography.The resulting purified antibodies were evaluated in a nephelometric system for the assay of transferrin in human sera. The results agreed closely with those obtained with a commercially available anti-human transferrin serum from rabbits.

AB - A rapid and economical procedure for extraction of antibodies from egg yolk is described. Laying hens were immunized with human transferrin and extracts of egg-yolk were purified with a procedure based on affinity chromatography.The resulting purified antibodies were evaluated in a nephelometric system for the assay of transferrin in human sera. The results agreed closely with those obtained with a commercially available anti-human transferrin serum from rabbits.

KW - B780-tropical-medicine

KW - Immunology

KW - Laboratory

KW - Antibodies

KW - Purification

KW - Chromatography

KW - Transferrin

KW - Nephelometry

KW - Egg-yolk

KW - IgY

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:A1992JM06800018

U2 - 10.1016/0022-1759(92)90315-K

DO - 10.1016/0022-1759(92)90315-K

M3 - A1: Web of Science-article

SN - 0022-1759

VL - 153

SP - 133

EP - 140

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

A simple and economical strategy for downstream processing of specific antibodies to human transferrin from egg yolk

Abstract

Keywords

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