A VSG expression site-associated gene confers resistance to human serum in Trypanosoma rhodesiense

HV Xong; L Vanhamme; M Chamekh; CE Chimfwembe; J Van Den Abbeele; A Pays; N Van Meirvenne; R Hamers; P De Baetselier; E Pays

A VSG expression site-associated gene confers resistance to human serum in Trypanosoma rhodesiense

HV Xong, L Vanhamme, M Chamekh, CE Chimfwembe, J Van Den Abbeele, A Pays, N Van Meirvenne, R Hamers, P De Baetselier, E Pays

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Infectivity of Trypanosoma brucei rhodesiense to humans is due to its resistance to a lytic factor present in human serum. In the ETat 1 strain this character was associated with antigenic variation, since expression of the ETat 1.10 variant surface glycoprotein was required to generate resistant (R) clones. In addition, in this strain transcription of a gene termed SRA was detected in R clones only. We show that the ETat 1.10 expression site is the one selectively transcribed in R variants. This expression site contains SRA as an expression site-associated gene (ESAG) and is characterized by the deletion of several ESAGs. Transfection of SRA into T.b. brucei was sufficient to confer resistance to human serum, identifying this gene as one of those responsible for T.b. rhodesiense adaptation to humans

Original language	English
Journal	Cell
Volume	95
Pages (from-to)	839-846
Publication status	Published - 1998

Keywords

B780-tropical-medicine
Protozoology
Trypanosoma brucei rhodesiense
Genetics
Immunology
Pathogenicity
Glycoproteins
Antigenic variation
Serum

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Cite this

@article{6ca9a8831027475aa45d1f254f816554,

title = "A VSG expression site-associated gene confers resistance to human serum in Trypanosoma rhodesiense",

abstract = "Infectivity of Trypanosoma brucei rhodesiense to humans is due to its resistance to a lytic factor present in human serum. In the ETat 1 strain this character was associated with antigenic variation, since expression of the ETat 1.10 variant surface glycoprotein was required to generate resistant (R) clones. In addition, in this strain transcription of a gene termed SRA was detected in R clones only. We show that the ETat 1.10 expression site is the one selectively transcribed in R variants. This expression site contains SRA as an expression site-associated gene (ESAG) and is characterized by the deletion of several ESAGs. Transfection of SRA into T.b. brucei was sufficient to confer resistance to human serum, identifying this gene as one of those responsible for T.b. rhodesiense adaptation to humans",

keywords = "B780-tropical-medicine, Protozoology, Trypanosoma brucei rhodesiense, Genetics, Immunology, Pathogenicity, Glycoproteins, Antigenic variation, Serum",

author = "HV Xong and L Vanhamme and M Chamekh and CE Chimfwembe and {Van Den Abbeele}, J and A Pays and {Van Meirvenne}, N and R Hamers and {De Baetselier}, P and E Pays",

note = "This item can be obtained at the ITG Library counter",

year = "1998",

language = "English",

volume = "95",

pages = "839--846",

journal = "Cell",

publisher = "Cell Press",

}

TY - JOUR

T1 - A VSG expression site-associated gene confers resistance to human serum in Trypanosoma rhodesiense

AU - Xong, HV

AU - Vanhamme, L

AU - Chamekh, M

AU - Chimfwembe, CE

AU - Van Den Abbeele, J

AU - Pays, A

AU - Van Meirvenne, N

AU - Hamers, R

AU - De Baetselier, P

AU - Pays, E

N1 - This item can be obtained at the ITG Library counter

PY - 1998

Y1 - 1998

N2 - Infectivity of Trypanosoma brucei rhodesiense to humans is due to its resistance to a lytic factor present in human serum. In the ETat 1 strain this character was associated with antigenic variation, since expression of the ETat 1.10 variant surface glycoprotein was required to generate resistant (R) clones. In addition, in this strain transcription of a gene termed SRA was detected in R clones only. We show that the ETat 1.10 expression site is the one selectively transcribed in R variants. This expression site contains SRA as an expression site-associated gene (ESAG) and is characterized by the deletion of several ESAGs. Transfection of SRA into T.b. brucei was sufficient to confer resistance to human serum, identifying this gene as one of those responsible for T.b. rhodesiense adaptation to humans

AB - Infectivity of Trypanosoma brucei rhodesiense to humans is due to its resistance to a lytic factor present in human serum. In the ETat 1 strain this character was associated with antigenic variation, since expression of the ETat 1.10 variant surface glycoprotein was required to generate resistant (R) clones. In addition, in this strain transcription of a gene termed SRA was detected in R clones only. We show that the ETat 1.10 expression site is the one selectively transcribed in R variants. This expression site contains SRA as an expression site-associated gene (ESAG) and is characterized by the deletion of several ESAGs. Transfection of SRA into T.b. brucei was sufficient to confer resistance to human serum, identifying this gene as one of those responsible for T.b. rhodesiense adaptation to humans

KW - B780-tropical-medicine

KW - Protozoology

KW - Trypanosoma brucei rhodesiense

KW - Genetics

KW - Immunology

KW - Pathogenicity

KW - Glycoproteins

KW - Antigenic variation

KW - Serum

M3 - A1: Web of Science-article

VL - 95

SP - 839

EP - 846

JO - Cell

JF - Cell

ER -

A VSG expression site-associated gene confers resistance to human serum in Trypanosoma rhodesiense

Abstract

Keywords

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