A VSG expression site-associated gene confers resistance to human serum in Trypanosoma rhodesiense

HV Xong, L Vanhamme, M Chamekh, CE Chimfwembe, J Van Den Abbeele, A Pays, N Van Meirvenne, R Hamers, P De Baetselier, E Pays

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    Infectivity of Trypanosoma brucei rhodesiense to humans is due to its resistance to a lytic factor present in human serum. In the ETat 1 strain this character was associated with antigenic variation, since expression of the ETat 1.10 variant surface glycoprotein was required to generate resistant (R) clones. In addition, in this strain transcription of a gene termed SRA was detected in R clones only. We show that the ETat 1.10 expression site is the one selectively transcribed in R variants. This expression site contains SRA as an expression site-associated gene (ESAG) and is characterized by the deletion of several ESAGs. Transfection of SRA into T.b. brucei was sufficient to confer resistance to human serum, identifying this gene as one of those responsible for T.b. rhodesiense adaptation to humans
    Original languageEnglish
    JournalCell
    Volume95
    Pages (from-to)839-846
    Publication statusPublished - 1998

    Keywords

    • B780-tropical-medicine
    • Protozoology
    • Trypanosoma brucei rhodesiense
    • Genetics
    • Immunology
    • Pathogenicity
    • Glycoproteins
    • Antigenic variation
    • Serum

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