Accordance and concordance of PCR and NASBA followed by oligochromatography for the molecular diagnosis of Trypanosoma brucei and Leishmania

CM Mugasa, S Deborggraeve, GJ Schoone, T Laurent, MM Leeflang, RA Ekangu, S El Safi, AF Saad, FL Basiye, S De Doncker, GW Lubega, PA Kager, P Büscher, HD Schallig

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    Summary Objective To evaluate the repeatability and reproducibility of four simplified molecular assays for the diagnosis of Trypanosoma brucei spp. or Leishmania ssp. in a multicentre ring trial with seven participating laboratories. Methods The tests are based on PCR or NASBA amplification of the parasites nucleic acids followed by rapid read-out by oligochromatographic dipstick (PCR-OC and NASBA-OC). Results On purified nucleic acid specimens, the repeatability and reproducibility of the tests were Tryp-PRC-OC, 91.7% and 95.5%; Tryp-NASBA-OC, 95.8% and 100%; Leish-PCR-OC, 95.9% and 98.1%; Leish-NASBA-OC, 92.3% and 98.2%. On blood specimens spiked with parasites, the repeatability and reproducibility of the tests were Tryp-PRC-OC, 78.4% and 86.6%; Tryp-NASBA-OC, 81.5% and 89.0%; Leish-PCR-OC, 87.1% and 91.7%; Leish-NASBA-OC, 74.8% and 86.2%. Conclusion As repeatability and reproducibility of the tests were satisfactory, further phase II and III evaluations in clinical and population specimens from disease endemic countries are justified
    Original languageEnglish
    JournalTropical Medicine and International Health
    Volume15
    Issue number7
    Pages (from-to)800-805
    Number of pages6
    ISSN1360-2276
    DOIs
    Publication statusPublished - 2010

    Keywords

    • B780-tropical-medicine
    • Protozoal diseases
    • Trypanosomiasis-African
    • Trypanosoma brucei
    • Leishmaniasis
    • Leishmania
    • Vectors
    • Tsetse flies
    • Glossina
    • Sandflies
    • Diagnosis
    • Molecular diagnostic techniques
    • Polymerase chain reaction
    • PCR
    • NASBA
    • Repeatability
    • Reproducibility
    • Assays
    • Oligochromatography
    • Dipstick
    • Laboratory techniques and procedures

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