Accordance and concordance of PCR and NASBA followed by oligochromatography for the molecular diagnosis of Trypanosoma brucei and Leishmania

CM Mugasa; S Deborggraeve; GJ Schoone; T Laurent; MM Leeflang; RA Ekangu; S El Safi; AF Saad; FL Basiye; S De Doncker; GW Lubega; PA Kager; P Büscher; HD Schallig

doi:10.1111/j.1365-3156.2010.02547.x

Accordance and concordance of PCR and NASBA followed by oligochromatography for the molecular diagnosis of Trypanosoma brucei and Leishmania

CM Mugasa, S Deborggraeve, GJ Schoone, T Laurent, MM Leeflang, RA Ekangu, S El Safi, AF Saad, FL Basiye, S De Doncker, GW Lubega, PA Kager, P Büscher, HD Schallig

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Objective To evaluate the repeatability and reproducibility of four simplified molecular assays for the diagnosis of Trypanosoma brucei spp. or Leishmania ssp. in a multicentre ring trial with seven participating laboratories.
Methods The tests are based on PCR or NASBA amplification of the parasites nucleic acids followed by rapid read-out by oligochromatographic dipstick (PCR-OC and NASBA-OC).
Results On purified nucleic acid specimens, the repeatability and reproducibility of the tests were Tryp-PRC-OC, 91.7% and 95.5%; Tryp-NASBA-OC, 95.8% and 100%; Leish-PCR-OC, 95.9% and 98.1%; Leish-NASBA-OC, 92.3% and 98.2%. On blood specimens spiked with parasites, the repeatability and reproducibility of the tests were Tryp-PRC-OC, 78.4% and 86.6%; Tryp-NASBA-OC, 81.5% and 89.0%; Leish-PCR-OC, 87.1% and 91.7%; Leish-NASBA-OC, 74.8% and 86.2%. Conclusion As repeatability and reproducibility of the tests were satisfactory, further phase II and III evaluations in clinical and population specimens from disease endemic countries are justified

Original language	English
Journal	Tropical Medicine and International Health
Volume	15
Issue number	7
Pages (from-to)	800-805
Number of pages	6
ISSN	1360-2276
DOIs	https://doi.org/10.1111/j.1365-3156.2010.02547.x
Publication status	Published - 2010

Keywords

B780-tropical-medicine
Protozoal diseases
Trypanosomiasis-African
Trypanosoma brucei
Leishmaniasis
Leishmania
Vectors
Tsetse flies
Glossina
Sandflies
Diagnosis
Molecular diagnostic techniques
Polymerase chain reaction
PCR
NASBA
Repeatability
Reproducibility
Assays
Oligochromatography
Dipstick
Laboratory techniques and procedures

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Mugasa, CM., Deborggraeve, S., Schoone, GJ., Laurent, T., Leeflang, MM., Ekangu, RA., El Safi, S., Saad, AF., Basiye, FL., De Doncker, S., Lubega, GW., Kager, PA., Büscher, P., & Schallig, HD. (2010). Accordance and concordance of PCR and NASBA followed by oligochromatography for the molecular diagnosis of Trypanosoma brucei and Leishmania. Tropical Medicine and International Health, 15(7), 800-805. https://doi.org/10.1111/j.1365-3156.2010.02547.x

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title = "Accordance and concordance of PCR and NASBA followed by oligochromatography for the molecular diagnosis of Trypanosoma brucei and Leishmania",

abstract = "Objective To evaluate the repeatability and reproducibility of four simplified molecular assays for the diagnosis of Trypanosoma brucei spp. or Leishmania ssp. in a multicentre ring trial with seven participating laboratories. Methods The tests are based on PCR or NASBA amplification of the parasites nucleic acids followed by rapid read-out by oligochromatographic dipstick (PCR-OC and NASBA-OC). Results On purified nucleic acid specimens, the repeatability and reproducibility of the tests were Tryp-PRC-OC, 91.7% and 95.5%; Tryp-NASBA-OC, 95.8% and 100%; Leish-PCR-OC, 95.9% and 98.1%; Leish-NASBA-OC, 92.3% and 98.2%. On blood specimens spiked with parasites, the repeatability and reproducibility of the tests were Tryp-PRC-OC, 78.4% and 86.6%; Tryp-NASBA-OC, 81.5% and 89.0%; Leish-PCR-OC, 87.1% and 91.7%; Leish-NASBA-OC, 74.8% and 86.2%. Conclusion As repeatability and reproducibility of the tests were satisfactory, further phase II and III evaluations in clinical and population specimens from disease endemic countries are justified",

keywords = "B780-tropical-medicine, Protozoal diseases, Trypanosomiasis-African, Trypanosoma brucei, Leishmaniasis, Leishmania, Vectors, Tsetse flies, Glossina, Sandflies, Diagnosis, Molecular diagnostic techniques, Polymerase chain reaction, PCR, NASBA, Repeatability, Reproducibility, Assays, Oligochromatography, Dipstick, Laboratory techniques and procedures",

author = "CM Mugasa and S Deborggraeve and GJ Schoone and T Laurent and MM Leeflang and RA Ekangu and {El Safi}, S and AF Saad and FL Basiye and {De Doncker}, S and GW Lubega and PA Kager and P B{\"u}scher and HD Schallig",

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year = "2010",

doi = "10.1111/j.1365-3156.2010.02547.x",

language = "English",

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pages = "800--805",

journal = "Tropical Medicine and International Health",

issn = "1360-2276",

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Mugasa, CM, Deborggraeve, S, Schoone, GJ, Laurent, T, Leeflang, MM, Ekangu, RA, El Safi, S, Saad, AF, Basiye, FL, De Doncker, S, Lubega, GW, Kager, PA, Büscher, P & Schallig, HD 2010, 'Accordance and concordance of PCR and NASBA followed by oligochromatography for the molecular diagnosis of Trypanosoma brucei and Leishmania', Tropical Medicine and International Health, vol. 15, no. 7, pp. 800-805. https://doi.org/10.1111/j.1365-3156.2010.02547.x

TY - JOUR

T1 - Accordance and concordance of PCR and NASBA followed by oligochromatography for the molecular diagnosis of Trypanosoma brucei and Leishmania

AU - Mugasa, CM

AU - Deborggraeve, S

AU - Schoone, GJ

AU - Laurent, T

AU - Leeflang, MM

AU - Ekangu, RA

AU - El Safi, S

AU - Saad, AF

AU - Basiye, FL

AU - De Doncker, S

AU - Lubega, GW

AU - Kager, PA

AU - Büscher, P

AU - Schallig, HD

N1 - FTX abonnement

PY - 2010

Y1 - 2010

N2 - Objective To evaluate the repeatability and reproducibility of four simplified molecular assays for the diagnosis of Trypanosoma brucei spp. or Leishmania ssp. in a multicentre ring trial with seven participating laboratories. Methods The tests are based on PCR or NASBA amplification of the parasites nucleic acids followed by rapid read-out by oligochromatographic dipstick (PCR-OC and NASBA-OC). Results On purified nucleic acid specimens, the repeatability and reproducibility of the tests were Tryp-PRC-OC, 91.7% and 95.5%; Tryp-NASBA-OC, 95.8% and 100%; Leish-PCR-OC, 95.9% and 98.1%; Leish-NASBA-OC, 92.3% and 98.2%. On blood specimens spiked with parasites, the repeatability and reproducibility of the tests were Tryp-PRC-OC, 78.4% and 86.6%; Tryp-NASBA-OC, 81.5% and 89.0%; Leish-PCR-OC, 87.1% and 91.7%; Leish-NASBA-OC, 74.8% and 86.2%. Conclusion As repeatability and reproducibility of the tests were satisfactory, further phase II and III evaluations in clinical and population specimens from disease endemic countries are justified

AB - Objective To evaluate the repeatability and reproducibility of four simplified molecular assays for the diagnosis of Trypanosoma brucei spp. or Leishmania ssp. in a multicentre ring trial with seven participating laboratories. Methods The tests are based on PCR or NASBA amplification of the parasites nucleic acids followed by rapid read-out by oligochromatographic dipstick (PCR-OC and NASBA-OC). Results On purified nucleic acid specimens, the repeatability and reproducibility of the tests were Tryp-PRC-OC, 91.7% and 95.5%; Tryp-NASBA-OC, 95.8% and 100%; Leish-PCR-OC, 95.9% and 98.1%; Leish-NASBA-OC, 92.3% and 98.2%. On blood specimens spiked with parasites, the repeatability and reproducibility of the tests were Tryp-PRC-OC, 78.4% and 86.6%; Tryp-NASBA-OC, 81.5% and 89.0%; Leish-PCR-OC, 87.1% and 91.7%; Leish-NASBA-OC, 74.8% and 86.2%. Conclusion As repeatability and reproducibility of the tests were satisfactory, further phase II and III evaluations in clinical and population specimens from disease endemic countries are justified

KW - B780-tropical-medicine

KW - Protozoal diseases

KW - Trypanosomiasis-African

KW - Trypanosoma brucei

KW - Leishmaniasis

KW - Leishmania

KW - Vectors

KW - Tsetse flies

KW - Glossina

KW - Sandflies

KW - Diagnosis

KW - Molecular diagnostic techniques

KW - Polymerase chain reaction

KW - PCR

KW - NASBA

KW - Repeatability

KW - Reproducibility

KW - Assays

KW - Oligochromatography

KW - Dipstick

KW - Laboratory techniques and procedures

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000279245800003

U2 - 10.1111/j.1365-3156.2010.02547.x

DO - 10.1111/j.1365-3156.2010.02547.x

M3 - A1: Web of Science-article

C2 - 20487429

SN - 1360-2276

VL - 15

SP - 800

EP - 805

JO - Tropical Medicine and International Health

JF - Tropical Medicine and International Health

IS - 7

ER -

Accordance and concordance of PCR and NASBA followed by oligochromatography for the molecular diagnosis of Trypanosoma brucei and Leishmania

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Keywords

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