African 2; a clonal complex of Mycobacterium bovis epidemiologically important in East Africa

S Berg, MC Garcia-Pelayo, B Müller, E Hailu, B Asiimwe, K Kremer, J Dale, B Boniotti, S Rodriguez, M Hilty, L Rigouts, R Firdessa, A Machado, C Mucavele, BNR Ngandolo, J Bruchfeld, L Boschiroli, A Müller, N Sahraoui, M PacciariniS Cadmus, M Joloba, D van Soolingen, AL Michel, B Djonne, A Aranaz, J Zinsstag, P van Helden, F Portaels, R Kazwala, G Kallenius, RG Hewinson, A Aseffa, SV Gordon, NH Smith

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    We have identified a clonal complex of Mycobacterium bovis isolated at high frequency from cattle in Uganda, Burundi, Tanzania, and Ethiopia. We have named this related group of M. bovis strains the African 2 (Af2) clonal complex of M. bovis. Af2 strains are defined by a specific chromosomal deletion (RDAf2) and can be identified by the absence of spacers 3 to 7 in their spoligotype patterns. Deletion analysis of M. bovis isolates from Algeria, Mali, Chad, Nigeria, Cameroon, South Africa, and Mozambique did not identify any strains of the Af2 clonal complex, suggesting that this clonal complex of M. bovis is localised in East Africa. The specific spoligotype pattern of the Af2 clonal complex was rarely identified among isolates from outside Africa and the few isolates that were found and tested were intact at the RDAf2 locus. We conclude that the Af2 clonal complex is localised to cattle in East Africa. We found that strains of the Af2 clonal complex of M. bovis have, in general, four or more copies of the insertion sequence IS6110 in contrast to the majority of M. bovis strains isolated from cattle that are thought to carry only one or a few copies.
    Original languageEnglish
    JournalJournal of Bacteriology
    Volume193
    Issue number3
    Pages (from-to)670-678
    ISSN0021-9193
    DOIs
    Publication statusPublished - 2011

    Keywords

    • B780-tropical-medicine
    • Bacterial diseases
    • Cattle
    • Mycobacterium bovis
    • Molecular epidemiology
    • Strains
    • Geographical distribution
    • Evolutionary genetics
    • Spoligotyping
    • Africa-East

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