African 2; a clonal complex of Mycobacterium bovis epidemiologically important in East Africa

S Berg; MC Garcia-Pelayo; B Müller; E Hailu; B Asiimwe; K Kremer; J Dale; B Boniotti; S Rodriguez; M Hilty; L Rigouts; R Firdessa; A Machado; C Mucavele; BNR Ngandolo; J Bruchfeld; L Boschiroli; A Müller; N Sahraoui; M Pacciarini; S Cadmus; M Joloba; D van Soolingen; AL Michel; B Djonne; A Aranaz; J Zinsstag; P van Helden; F Portaels; R Kazwala; G Kallenius; RG Hewinson; A Aseffa; SV Gordon; NH Smith

doi:10.1128/JB.00750-10

African 2; a clonal complex of Mycobacterium bovis epidemiologically important in East Africa

S Berg, MC Garcia-Pelayo, B Müller, E Hailu, B Asiimwe, K Kremer, J Dale, B Boniotti, S Rodriguez, M Hilty, L Rigouts, R Firdessa, A Machado, C Mucavele, BNR Ngandolo, J Bruchfeld, L Boschiroli, A Müller, N Sahraoui, M PacciariniS Cadmus, M Joloba, D van Soolingen, AL Michel, B Djonne, A Aranaz, J Zinsstag, P van Helden, F Portaels, R Kazwala, G Kallenius, RG Hewinson, A Aseffa, SV Gordon, NH Smith

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Abstract

We have identified a clonal complex of Mycobacterium bovis isolated at high frequency from cattle in Uganda, Burundi, Tanzania, and Ethiopia. We have named this related group of M. bovis strains the African 2 (Af2) clonal complex of M. bovis. Af2 strains are defined by a specific chromosomal deletion (RDAf2) and can be identified by the absence of spacers 3 to 7 in their spoligotype patterns. Deletion analysis of M. bovis isolates from Algeria, Mali, Chad, Nigeria, Cameroon, South Africa, and Mozambique did not identify any strains of the Af2 clonal complex, suggesting that this clonal complex of M. bovis is localised in East Africa. The specific spoligotype pattern of the Af2 clonal complex was rarely identified among isolates from outside Africa and the few isolates that were found and tested were intact at the RDAf2 locus. We conclude that the Af2 clonal complex is localised to cattle in East Africa. We found that strains of the Af2 clonal complex of M. bovis have, in general, four or more copies of the insertion sequence IS6110 in contrast to the majority of M. bovis strains isolated from cattle that are thought to carry only one or a few copies.

Original language	English
Journal	Journal of Bacteriology
Volume	193
Issue number	3
Pages (from-to)	670-678
ISSN	0021-9193
DOIs	https://doi.org/10.1128/JB.00750-10
Publication status	Published - 2011

Keywords

B780-tropical-medicine
Bacterial diseases
Cattle
Mycobacterium bovis
Molecular epidemiology
Strains
Geographical distribution
Evolutionary genetics
Spoligotyping
Africa-East

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10.1128/JB.00750-10

2011jbac0670

http://lib.itg.be/pdf/itg/2011/2011jbac0670.pdf

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Berg, S., Garcia-Pelayo, MC., Müller, B., Hailu, E., Asiimwe, B., Kremer, K., Dale, J., Boniotti, B., Rodriguez, S., Hilty, M., Rigouts, L., Firdessa, R., Machado, A., Mucavele, C., Ngandolo, BNR., Bruchfeld, J., Boschiroli, L., Müller, A., Sahraoui, N., ... Smith, NH. (2011). African 2; a clonal complex of Mycobacterium bovis epidemiologically important in East Africa. Journal of Bacteriology, 193(3), 670-678. https://doi.org/10.1128/JB.00750-10

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title = "African 2; a clonal complex of Mycobacterium bovis epidemiologically important in East Africa",

abstract = "We have identified a clonal complex of Mycobacterium bovis isolated at high frequency from cattle in Uganda, Burundi, Tanzania, and Ethiopia. We have named this related group of M. bovis strains the African 2 (Af2) clonal complex of M. bovis. Af2 strains are defined by a specific chromosomal deletion (RDAf2) and can be identified by the absence of spacers 3 to 7 in their spoligotype patterns. Deletion analysis of M. bovis isolates from Algeria, Mali, Chad, Nigeria, Cameroon, South Africa, and Mozambique did not identify any strains of the Af2 clonal complex, suggesting that this clonal complex of M. bovis is localised in East Africa. The specific spoligotype pattern of the Af2 clonal complex was rarely identified among isolates from outside Africa and the few isolates that were found and tested were intact at the RDAf2 locus. We conclude that the Af2 clonal complex is localised to cattle in East Africa. We found that strains of the Af2 clonal complex of M. bovis have, in general, four or more copies of the insertion sequence IS6110 in contrast to the majority of M. bovis strains isolated from cattle that are thought to carry only one or a few copies.",

keywords = "B780-tropical-medicine, Bacterial diseases, Cattle, Mycobacterium bovis, Molecular epidemiology, Strains, Geographical distribution, Evolutionary genetics, Spoligotyping, Africa-East",

author = "S Berg and MC Garcia-Pelayo and B M{\"u}ller and E Hailu and B Asiimwe and K Kremer and J Dale and B Boniotti and S Rodriguez and M Hilty and L Rigouts and R Firdessa and A Machado and C Mucavele and BNR Ngandolo and J Bruchfeld and L Boschiroli and A M{\"u}ller and N Sahraoui and M Pacciarini and S Cadmus and M Joloba and {van Soolingen}, D and AL Michel and B Djonne and A Aranaz and J Zinsstag and {van Helden}, P and F Portaels and R Kazwala and G Kallenius and RG Hewinson and A Aseffa and SV Gordon and NH Smith",

note = "FTX; ITG-M11A; ITG-M29A; MICRO; U-MYCOB; JIF; DOI; PDF; Abstract; DSPACE",

year = "2011",

doi = "10.1128/JB.00750-10",

language = "English",

volume = "193",

pages = "670--678",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "3",

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Berg, S, Garcia-Pelayo, MC, Müller, B, Hailu, E, Asiimwe, B, Kremer, K, Dale, J, Boniotti, B, Rodriguez, S, Hilty, M, Rigouts, L, Firdessa, R, Machado, A, Mucavele, C, Ngandolo, BNR, Bruchfeld, J, Boschiroli, L, Müller, A, Sahraoui, N, Pacciarini, M, Cadmus, S, Joloba, M, van Soolingen, D, Michel, AL, Djonne, B, Aranaz, A, Zinsstag, J, van Helden, P, Portaels, F, Kazwala, R, Kallenius, G, Hewinson, RG, Aseffa, A, Gordon, SV & Smith, NH 2011, 'African 2; a clonal complex of Mycobacterium bovis epidemiologically important in East Africa', Journal of Bacteriology, vol. 193, no. 3, pp. 670-678. https://doi.org/10.1128/JB.00750-10

TY - JOUR

T1 - African 2; a clonal complex of Mycobacterium bovis epidemiologically important in East Africa

AU - Berg, S

AU - Garcia-Pelayo, MC

AU - Müller, B

AU - Hailu, E

AU - Asiimwe, B

AU - Kremer, K

AU - Dale, J

AU - Boniotti, B

AU - Rodriguez, S

AU - Hilty, M

AU - Rigouts, L

AU - Firdessa, R

AU - Machado, A

AU - Mucavele, C

AU - Ngandolo, BNR

AU - Bruchfeld, J

AU - Boschiroli, L

AU - Müller, A

AU - Sahraoui, N

AU - Pacciarini, M

AU - Cadmus, S

AU - Joloba, M

AU - van Soolingen, D

AU - Michel, AL

AU - Djonne, B

AU - Aranaz, A

AU - Zinsstag, J

AU - van Helden, P

AU - Portaels, F

AU - Kazwala, R

AU - Kallenius, G

AU - Hewinson, RG

AU - Aseffa, A

AU - Gordon, SV

AU - Smith, NH

N1 - FTX; ITG-M11A; ITG-M29A; MICRO; U-MYCOB; JIF; DOI; PDF; Abstract; DSPACE

PY - 2011

Y1 - 2011

N2 - We have identified a clonal complex of Mycobacterium bovis isolated at high frequency from cattle in Uganda, Burundi, Tanzania, and Ethiopia. We have named this related group of M. bovis strains the African 2 (Af2) clonal complex of M. bovis. Af2 strains are defined by a specific chromosomal deletion (RDAf2) and can be identified by the absence of spacers 3 to 7 in their spoligotype patterns. Deletion analysis of M. bovis isolates from Algeria, Mali, Chad, Nigeria, Cameroon, South Africa, and Mozambique did not identify any strains of the Af2 clonal complex, suggesting that this clonal complex of M. bovis is localised in East Africa. The specific spoligotype pattern of the Af2 clonal complex was rarely identified among isolates from outside Africa and the few isolates that were found and tested were intact at the RDAf2 locus. We conclude that the Af2 clonal complex is localised to cattle in East Africa. We found that strains of the Af2 clonal complex of M. bovis have, in general, four or more copies of the insertion sequence IS6110 in contrast to the majority of M. bovis strains isolated from cattle that are thought to carry only one or a few copies.

AB - We have identified a clonal complex of Mycobacterium bovis isolated at high frequency from cattle in Uganda, Burundi, Tanzania, and Ethiopia. We have named this related group of M. bovis strains the African 2 (Af2) clonal complex of M. bovis. Af2 strains are defined by a specific chromosomal deletion (RDAf2) and can be identified by the absence of spacers 3 to 7 in their spoligotype patterns. Deletion analysis of M. bovis isolates from Algeria, Mali, Chad, Nigeria, Cameroon, South Africa, and Mozambique did not identify any strains of the Af2 clonal complex, suggesting that this clonal complex of M. bovis is localised in East Africa. The specific spoligotype pattern of the Af2 clonal complex was rarely identified among isolates from outside Africa and the few isolates that were found and tested were intact at the RDAf2 locus. We conclude that the Af2 clonal complex is localised to cattle in East Africa. We found that strains of the Af2 clonal complex of M. bovis have, in general, four or more copies of the insertion sequence IS6110 in contrast to the majority of M. bovis strains isolated from cattle that are thought to carry only one or a few copies.

KW - B780-tropical-medicine

KW - Bacterial diseases

KW - Cattle

KW - Mycobacterium bovis

KW - Molecular epidemiology

KW - Strains

KW - Geographical distribution

KW - Evolutionary genetics

KW - Spoligotyping

KW - Africa-East

U2 - 10.1128/JB.00750-10

DO - 10.1128/JB.00750-10

M3 - A1: Web of Science-article

C2 - 21097608

SN - 0021-9193

VL - 193

SP - 670

EP - 678

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 3

ER -

African 2; a clonal complex of Mycobacterium bovis epidemiologically important in East Africa

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Keywords

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