All Staphylococcus aureus bacteraemia-inducing strains can cause infective endocarditis: results of GWAS and experimental animal studies

Sylvère Bastien, Severien Meyers, Wilmara Salgado-Pabón, Stefano G Giulieri, Jean-Phillipe Rasigade, Laurens Liesenborghs, Kyle J Kinney, Florence Couzon, Patricia Martins-Simoes, Vincent Le Moing, Xavier Duval, Natasha E Holmes, Niels Eske Bruun, Robert Skov, Benjamin P Howden, Vance G Fowler, Peter Verhamme, Paal Skytt Andersen, Coralie Bouchiat, Karen MoreauFrançois Vandenesch

Research output: Contribution to journalA1: Web of Science-articlepeer-review

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OBJECTIVES: We aimed at determining whether specific S. aureus strains cause infective endocarditis (IE) in the course of Staphylococcus aureus bacteraemia (SAB).

METHODS: A genome-wide association study (GWAS) including 924 S. aureus genomes from IE (274) and non-IE (650) SAB patients from international cohorts was conducted, and a subset of strains was tested with two experimental animal models of IE, one investigating the early step of bacterial adhesion to inflamed mice valves, the second evaluating the local and systemic developmental process of IE on mechanically-damaged rabbit valves.

RESULTS: The genetic profile of S. aureus IE and non-IE SAB strains did not differ when considering single nucleotide polymorphisms, coding sequences, and k-mers analysed in GWAS. In the murine inflammation-induced IE model, no difference was observed between IE and non-IE SAB strains both in terms of adhesion to the cardiac valves and in the propensity to cause IE; in the mechanical IE-induced rabbit model, there was no difference between IE and non-IE SAB strains regarding the vegetation size and CFU.

CONCLUSION: All strains of S. aureus isolated from SAB patients must be considered as capable of causing this common and lethal infection once they have accessed the bloodstream.

Original languageEnglish
JournalJournal of Infection
Issue number2
Pages (from-to)123-133
Number of pages11
Publication statusPublished - 2023
Externally publishedYes


  • Animals
  • Rabbits
  • Mice
  • Genome-Wide Association Study
  • Bacteremia/microbiology
  • Staphylococcal Infections/microbiology
  • Staphylococcus aureus/genetics
  • Endocarditis, Bacterial/microbiology
  • Endocarditis/microbiology

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