American tegumentary leishmaniasis: direct species identification of Leishmania in non-invasive clinical samples

AL Garcia; R Parrado; S De Doncker; H Bermudez; JC Dujardin

doi:10.1016/j.trstmh.2006.06.009

American tegumentary leishmaniasis: direct species identification of Leishmania in non-invasive clinical samples

AL Garcia, R Parrado, S De Doncker, H Bermudez, JC Dujardin

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Species identification is highly relevant for improved prognosis and adequate treatment of American tegumentary leishmaniasis (ATL). PCR-based methods are available for this purpose but should be simplified to improve accessibility. As a first step in this process, this paper describes a simplified protocol for collection of clinical samples. Using samples from 44 Bolivian patients with confirmed ATL, we demonstrated that hsp70 PCR-RFLP on skin scrapings collected with a tooth pick allowed identification of the parasite species with a sensitivity of 95% and specificity of 100%. Our method should greatly facilitate individual patient management and epidemiological surveillance of ATL. (C) 2006 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.

Original language	English
Journal	Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume	101
Issue number	4
Pages (from-to)	368-371
ISSN	0035-9203
DOIs	https://doi.org/10.1016/j.trstmh.2006.06.009
Publication status	Published - 2007

Keywords

B780-tropical-medicine
Protozoal diseases
Leishmaniasis
Tegumentary
Cutaneous
Species
Leishmania braziliensis
Leishmania lainsoni
Identification
Molecular diagnostic techniques
PCR
Simplified
Sensitivity
Specificity
Bolivia
America-Latin

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10.1016/j.trstmh.2006.06.009

http://lib.itg.be/pdf/itg/2007/2007trst0368.pdf

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title = "American tegumentary leishmaniasis: direct species identification of Leishmania in non-invasive clinical samples",

abstract = "Species identification is highly relevant for improved prognosis and adequate treatment of American tegumentary leishmaniasis (ATL). PCR-based methods are available for this purpose but should be simplified to improve accessibility. As a first step in this process, this paper describes a simplified protocol for collection of clinical samples. Using samples from 44 Bolivian patients with confirmed ATL, we demonstrated that hsp70 PCR-RFLP on skin scrapings collected with a tooth pick allowed identification of the parasite species with a sensitivity of 95% and specificity of 100%. Our method should greatly facilitate individual patient management and epidemiological surveillance of ATL. (C) 2006 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.",

keywords = "B780-tropical-medicine, Protozoal diseases, Leishmaniasis, Tegumentary, Cutaneous, Species, Leishmania braziliensis, Leishmania lainsoni, Identification, Molecular diagnostic techniques, PCR, Simplified, Sensitivity, Specificity, Bolivia, America-Latin",

author = "AL Garcia and R Parrado and {De Doncker}, S and H Bermudez and JC Dujardin",

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T1 - American tegumentary leishmaniasis: direct species identification of Leishmania in non-invasive clinical samples

AU - Garcia, AL

AU - Parrado, R

AU - De Doncker, S

AU - Bermudez, H

AU - Dujardin, JC

N1 - FTX abonnement

PY - 2007

Y1 - 2007

N2 - Species identification is highly relevant for improved prognosis and adequate treatment of American tegumentary leishmaniasis (ATL). PCR-based methods are available for this purpose but should be simplified to improve accessibility. As a first step in this process, this paper describes a simplified protocol for collection of clinical samples. Using samples from 44 Bolivian patients with confirmed ATL, we demonstrated that hsp70 PCR-RFLP on skin scrapings collected with a tooth pick allowed identification of the parasite species with a sensitivity of 95% and specificity of 100%. Our method should greatly facilitate individual patient management and epidemiological surveillance of ATL. (C) 2006 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.

AB - Species identification is highly relevant for improved prognosis and adequate treatment of American tegumentary leishmaniasis (ATL). PCR-based methods are available for this purpose but should be simplified to improve accessibility. As a first step in this process, this paper describes a simplified protocol for collection of clinical samples. Using samples from 44 Bolivian patients with confirmed ATL, we demonstrated that hsp70 PCR-RFLP on skin scrapings collected with a tooth pick allowed identification of the parasite species with a sensitivity of 95% and specificity of 100%. Our method should greatly facilitate individual patient management and epidemiological surveillance of ATL. (C) 2006 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.

KW - B780-tropical-medicine

KW - Protozoal diseases

KW - Leishmaniasis

KW - Tegumentary

KW - Cutaneous

KW - Species

KW - Leishmania braziliensis

KW - Leishmania lainsoni

KW - Identification

KW - Molecular diagnostic techniques

KW - PCR

KW - Simplified

KW - Sensitivity

KW - Specificity

KW - Bolivia

KW - America-Latin

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U2 - 10.1016/j.trstmh.2006.06.009

DO - 10.1016/j.trstmh.2006.06.009

M3 - A1: Web of Science-article

SN - 0035-9203

VL - 101

SP - 368

EP - 371

JO - Transactions of the Royal Society of Tropical Medicine and Hygiene

JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

IS - 4

ER -

American tegumentary leishmaniasis: direct species identification of Leishmania in non-invasive clinical samples

Abstract

Keywords

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