An experimental latex agglutination test for antibody detection in human African trypanosomiasis

P Büscher; E Draelants; E Magnus; T Vervoort; N Van Meirvenne

An experimental latex agglutination test for antibody detection in human African trypanosomiasis

P Büscher, E Draelants, E Magnus, T Vervoort, N Van Meirvenne

Institute of Tropical Medicine

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

A latex card agglutination test for detection of antibodies in human African trypanosomiasis is presented. The latex was covalently coated with semipurified surface glycoprotein of Variable Antigen Type LiTat 1.6 of Trypanosoma brucei gambiense. Sera from 100 patients infected with T.b. gambiense, 26 patients infected with T.b. rhodesiense and 707 individuals without trypanosomiasis, including 132 malaria seropositives, have been tested. At serum dilution 1:16, sensitivity of the test was 91% for the T.b. gambiense and 42.3% for the T.b. rhodesiense group. Specificity was over 99%. The reagent remained stable at +/- 6 degrees C for at least 3 months. Reagent kept at 37 degrees C for 3 months retained its sensitivity and showed a slight decrease in specificity

Original language	English
Journal	Annales de la Société Belge de Médecine Tropicale
Volume	71
Pages (from-to)	267-273
ISSN	0365-6527
Publication status	Published - 1991

Keywords

B780-tropical-medicine
Protozoal diseases
Trypanosomiasis
Trypanosoma brucei gambiense
Trypanosoma brucei rhodesiense
Antibody detection
Latex fixation tests
Specificity
Sensitivity
Laboratory techniques and procedures

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Cite this

@article{1d54112df8174d82bb0001d81a3fbe58,

title = "An experimental latex agglutination test for antibody detection in human African trypanosomiasis",

abstract = "A latex card agglutination test for detection of antibodies in human African trypanosomiasis is presented. The latex was covalently coated with semipurified surface glycoprotein of Variable Antigen Type LiTat 1.6 of Trypanosoma brucei gambiense. Sera from 100 patients infected with T.b. gambiense, 26 patients infected with T.b. rhodesiense and 707 individuals without trypanosomiasis, including 132 malaria seropositives, have been tested. At serum dilution 1:16, sensitivity of the test was 91% for the T.b. gambiense and 42.3% for the T.b. rhodesiense group. Specificity was over 99%. The reagent remained stable at +/- 6 degrees C for at least 3 months. Reagent kept at 37 degrees C for 3 months retained its sensitivity and showed a slight decrease in specificity",

keywords = "B780-tropical-medicine, Protozoal diseases, Trypanosomiasis, Trypanosoma brucei gambiense, Trypanosoma brucei rhodesiense, Antibody detection, Latex fixation tests, Specificity, Sensitivity, Laboratory techniques and procedures",

author = "P B{\"u}scher and E Draelants and E Magnus and T Vervoort and {Van Meirvenne}, N",

year = "1991",

language = "English",

volume = "71",

pages = "267--273",

journal = "Annales de la Soci{\'e}t{\'e} Belge de M{\'e}decine Tropicale",

issn = "0365-6527",

publisher = "Acco",

}

TY - JOUR

T1 - An experimental latex agglutination test for antibody detection in human African trypanosomiasis

AU - Büscher, P

AU - Draelants, E

AU - Magnus, E

AU - Vervoort, T

AU - Van Meirvenne, N

PY - 1991

Y1 - 1991

N2 - A latex card agglutination test for detection of antibodies in human African trypanosomiasis is presented. The latex was covalently coated with semipurified surface glycoprotein of Variable Antigen Type LiTat 1.6 of Trypanosoma brucei gambiense. Sera from 100 patients infected with T.b. gambiense, 26 patients infected with T.b. rhodesiense and 707 individuals without trypanosomiasis, including 132 malaria seropositives, have been tested. At serum dilution 1:16, sensitivity of the test was 91% for the T.b. gambiense and 42.3% for the T.b. rhodesiense group. Specificity was over 99%. The reagent remained stable at +/- 6 degrees C for at least 3 months. Reagent kept at 37 degrees C for 3 months retained its sensitivity and showed a slight decrease in specificity

AB - A latex card agglutination test for detection of antibodies in human African trypanosomiasis is presented. The latex was covalently coated with semipurified surface glycoprotein of Variable Antigen Type LiTat 1.6 of Trypanosoma brucei gambiense. Sera from 100 patients infected with T.b. gambiense, 26 patients infected with T.b. rhodesiense and 707 individuals without trypanosomiasis, including 132 malaria seropositives, have been tested. At serum dilution 1:16, sensitivity of the test was 91% for the T.b. gambiense and 42.3% for the T.b. rhodesiense group. Specificity was over 99%. The reagent remained stable at +/- 6 degrees C for at least 3 months. Reagent kept at 37 degrees C for 3 months retained its sensitivity and showed a slight decrease in specificity

KW - B780-tropical-medicine

KW - Protozoal diseases

KW - Trypanosomiasis

KW - Trypanosoma brucei gambiense

KW - Trypanosoma brucei rhodesiense

KW - Antibody detection

KW - Latex fixation tests

KW - Specificity

KW - Sensitivity

KW - Laboratory techniques and procedures

M3 - A1: Web of Science-article

SN - 0365-6527

VL - 71

SP - 267

EP - 273

JO - Annales de la Société Belge de Médecine Tropicale

JF - Annales de la Société Belge de Médecine Tropicale

ER -

An experimental latex agglutination test for antibody detection in human African trypanosomiasis

Abstract

Keywords

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