An inhibition enzyme immunoassay using a human monoclonal antibody (K14) reactive with gp41 of HIV-1 for the serology of HIV-1 infections

VJP Teeuwsen; JJ Schalken; G van der Groen; R Van de Akker; J Goudsmit; ADME Osterhaus

doi:10.1097/00002030-199107000-00017

An inhibition enzyme immunoassay using a human monoclonal antibody (K14) reactive with gp41 of HIV-1 for the serology of HIV-1 infections

VJP Teeuwsen, JJ Schalken, G van der Groen, R Van de Akker, J Goudsmit, ADME Osterhaus

Institute of Tropical Medicine

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

An inhibition enzyme immunoassay (IEIA), using a human monoclonal antibody (K14) reactive with gp41 of HIV-1, was evaluated for its applicability to the serology of HIV-1 infections. Using panels of serum samples from seronegative and confirmed HIV-1-seropositive individuals, it was shown that all the HIV-1-positive samples in a panel from The Netherlands and 97% of the HIV-1-positive samples from Tanzania were identified by this IEIA. Six per cent of the IEIA-positive samples from Tanzania could not be confirmed in other assays. Testing of serial dilutions of serum samples from African individuals with confirmed HIV-1, HIV-2 or HIV ANT70 infections in the K14 IEIA, indicated that a HIV-1-specific assay based on this principle may be developed.

Original language	English
Journal	AIDS
Volume	5
Issue number	7
Pages (from-to)	893-896
ISSN	0269-9370
DOIs	https://doi.org/10.1097/00002030-199107000-00017
Publication status	Published - 1991

Keywords

B780-tropical-medicine
Virology
HIV-1
Serology
Immunoenzyme techniques
Inhibition enzyme immunoassay
Monoclonal antibodies
Laboratory techniques and procedures

Access to Document

10.1097/00002030-199107000-00017

Cite this

@article{0ceb40ed69a149b09c0e30b18e3f2fff,

title = "An inhibition enzyme immunoassay using a human monoclonal antibody (K14) reactive with gp41 of HIV-1 for the serology of HIV-1 infections",

abstract = "An inhibition enzyme immunoassay (IEIA), using a human monoclonal antibody (K14) reactive with gp41 of HIV-1, was evaluated for its applicability to the serology of HIV-1 infections. Using panels of serum samples from seronegative and confirmed HIV-1-seropositive individuals, it was shown that all the HIV-1-positive samples in a panel from The Netherlands and 97% of the HIV-1-positive samples from Tanzania were identified by this IEIA. Six per cent of the IEIA-positive samples from Tanzania could not be confirmed in other assays. Testing of serial dilutions of serum samples from African individuals with confirmed HIV-1, HIV-2 or HIV ANT70 infections in the K14 IEIA, indicated that a HIV-1-specific assay based on this principle may be developed.",

keywords = "B780-tropical-medicine, Virology, HIV-1, Serology, Immunoenzyme techniques, Inhibition enzyme immunoassay, Monoclonal antibodies, Laboratory techniques and procedures",

author = "VJP Teeuwsen and JJ Schalken and {van der Groen}, G and {Van de Akker}, R and J Goudsmit and ADME Osterhaus",

year = "1991",

doi = "10.1097/00002030-199107000-00017",

language = "English",

volume = "5",

pages = "893--896",

journal = "AIDS",

issn = "0269-9370",

publisher = "Lippincott Williams & Wilkins",

number = "7",

}

TY - JOUR

T1 - An inhibition enzyme immunoassay using a human monoclonal antibody (K14) reactive with gp41 of HIV-1 for the serology of HIV-1 infections

AU - Teeuwsen, VJP

AU - Schalken, JJ

AU - van der Groen, G

AU - Van de Akker, R

AU - Goudsmit, J

AU - Osterhaus, ADME

PY - 1991

Y1 - 1991

N2 - An inhibition enzyme immunoassay (IEIA), using a human monoclonal antibody (K14) reactive with gp41 of HIV-1, was evaluated for its applicability to the serology of HIV-1 infections. Using panels of serum samples from seronegative and confirmed HIV-1-seropositive individuals, it was shown that all the HIV-1-positive samples in a panel from The Netherlands and 97% of the HIV-1-positive samples from Tanzania were identified by this IEIA. Six per cent of the IEIA-positive samples from Tanzania could not be confirmed in other assays. Testing of serial dilutions of serum samples from African individuals with confirmed HIV-1, HIV-2 or HIV ANT70 infections in the K14 IEIA, indicated that a HIV-1-specific assay based on this principle may be developed.

AB - An inhibition enzyme immunoassay (IEIA), using a human monoclonal antibody (K14) reactive with gp41 of HIV-1, was evaluated for its applicability to the serology of HIV-1 infections. Using panels of serum samples from seronegative and confirmed HIV-1-seropositive individuals, it was shown that all the HIV-1-positive samples in a panel from The Netherlands and 97% of the HIV-1-positive samples from Tanzania were identified by this IEIA. Six per cent of the IEIA-positive samples from Tanzania could not be confirmed in other assays. Testing of serial dilutions of serum samples from African individuals with confirmed HIV-1, HIV-2 or HIV ANT70 infections in the K14 IEIA, indicated that a HIV-1-specific assay based on this principle may be developed.

KW - B780-tropical-medicine

KW - Virology

KW - HIV-1

KW - Serology

KW - Immunoenzyme techniques

KW - Inhibition enzyme immunoassay

KW - Monoclonal antibodies

KW - Laboratory techniques and procedures

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:A1991FX26400017

U2 - 10.1097/00002030-199107000-00017

DO - 10.1097/00002030-199107000-00017

M3 - A1: Web of Science-article

SN - 0269-9370

VL - 5

SP - 893

EP - 896

JO - AIDS

JF - AIDS

IS - 7

ER -

An inhibition enzyme immunoassay using a human monoclonal antibody (K14) reactive with gp41 of HIV-1 for the serology of HIV-1 infections

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this