An innovative tool for moving malaria PCR detection of parasite reservoir into the field

L. Canier; N. Khim; S. Kim; V. Sluydts; S. Heng; D. Dourng; R. Eam; S. Chy; C. Khean; K. Loch; M. Ken; H. Lim; S. Siv; S. Tho; P. Masse-Navette; C. Gryseels; S. Uk; K. Van Roey; K. Peeters Grietens; M. Sokny; B. Thavrin; C.M. Chuor; V. Deubel; L. Durnez; M. Coosemans; D. Menard

doi:10.1186/1475-2875-12-405

An innovative tool for moving malaria PCR detection of parasite reservoir into the field

L. Canier, N. Khim, S. Kim, V. Sluydts, S. Heng, D. Dourng, R. Eam, S. Chy, C. Khean, K. Loch, M. Ken, H. Lim, S. Siv, S. Tho, P. Masse-Navette, C. Gryseels, S. Uk, K. Van Roey, K. Peeters Grietens, M. SoknyB. Thavrin, C.M. Chuor, V. Deubel, L. Durnez, M. Coosemans, D. Menard

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Abstract

BACKGROUND: To achieve the goal of malaria elimination in low transmission areas such as in Cambodia, new, inexpensive, high-throughput diagnostic tools for identifying very low parasite densities in asymptomatic carriers are required. This will enable a switch from passive to active malaria case detection in the field. METHODS: DNA extraction and real-time PCR assays were implemented in an "in-house" designed mobile laboratory allowing implementation of a robust, sensitive and rapid malaria diagnostic strategy in the field. This tool was employed in a survey organized in the context of the MalaResT project (NCT01663831). RESULTS: The real-time PCR screening and species identification assays were performed in the mobile laboratory between October and November 2012, in Rattanakiri Province, to screen approximately 5,000 individuals in less than four weeks and treat parasite carriers within 24--48 hours after sample collection. An average of 240 clinical samples (and 40 quality control samples) was tested every day, six/seven days per week. Some 97.7% of the results were available

Original language	English
Journal	Malaria Journal
Volume	12
Issue number	405
Pages (from-to)	1-12
Number of pages	12
ISSN	1475-2875
DOIs	https://doi.org/10.1186/1475-2875-12-405
Publication status	Published - 2013

Keywords

Protozoal diseases
Malaria
Plasmodium falciparum
Plasmodium vivax
Plasmodium malariae
Plasmodium ovale
Vectors
Mosquitoes
Anopheles
Diagnosis
Detection
Rapid diagnostic tests
Real-time
Polymerase chain reaction
PCR
Tools
Development
Case detection
Carriers
Mobile
Cambodia
Asia-Southeast

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2013mjou0405Final published version, 703 KB

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Canier, L., Khim, N., Kim, S., Sluydts, V., Heng, S., Dourng, D., Eam, R., Chy, S., Khean, C., Loch, K., Ken, M., Lim, H., Siv, S., Tho, S., Masse-Navette, P., Gryseels, C., Uk, S., Van Roey, K., Peeters Grietens, K., ... Menard, D. (2013). An innovative tool for moving malaria PCR detection of parasite reservoir into the field. Malaria Journal, 12(405), 1-12. https://doi.org/10.1186/1475-2875-12-405

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abstract = "BACKGROUND: To achieve the goal of malaria elimination in low transmission areas such as in Cambodia, new, inexpensive, high-throughput diagnostic tools for identifying very low parasite densities in asymptomatic carriers are required. This will enable a switch from passive to active malaria case detection in the field. METHODS: DNA extraction and real-time PCR assays were implemented in an {"}in-house{"} designed mobile laboratory allowing implementation of a robust, sensitive and rapid malaria diagnostic strategy in the field. This tool was employed in a survey organized in the context of the MalaResT project (NCT01663831). RESULTS: The real-time PCR screening and species identification assays were performed in the mobile laboratory between October and November 2012, in Rattanakiri Province, to screen approximately 5,000 individuals in less than four weeks and treat parasite carriers within 24--48 hours after sample collection. An average of 240 clinical samples (and 40 quality control samples) was tested every day, six/seven days per week. Some 97.7% of the results were available ",

keywords = "Protozoal diseases, Malaria, Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, Plasmodium ovale, Vectors, Mosquitoes, Anopheles, Diagnosis, Detection, Rapid diagnostic tests, Real-time, Polymerase chain reaction, PCR, Tools, Development, Case detection, Carriers, Mobile, Cambodia, Asia-Southeast",

author = "L. Canier and N. Khim and S. Kim and V. Sluydts and S. Heng and D. Dourng and R. Eam and S. Chy and C. Khean and K. Loch and M. Ken and H. Lim and S. Siv and S. Tho and P. Masse-Navette and C. Gryseels and S. Uk and {Van Roey}, K. and {Peeters Grietens}, K. and M. Sokny and B. Thavrin and C.M. Chuor and V. Deubel and L. Durnez and M. Coosemans and D. Menard",

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Canier, L, Khim, N, Kim, S, Sluydts, V, Heng, S, Dourng, D, Eam, R, Chy, S, Khean, C, Loch, K, Ken, M, Lim, H, Siv, S, Tho, S, Masse-Navette, P, Gryseels, C, Uk, S, Van Roey, K, Peeters Grietens, K, Sokny, M, Thavrin, B, Chuor, CM, Deubel, V, Durnez, L, Coosemans, M & Menard, D 2013, 'An innovative tool for moving malaria PCR detection of parasite reservoir into the field', Malaria Journal, vol. 12, no. 405, pp. 1-12. https://doi.org/10.1186/1475-2875-12-405

TY - JOUR

T1 - An innovative tool for moving malaria PCR detection of parasite reservoir into the field

AU - Canier, L.

AU - Khim, N.

AU - Kim, S.

AU - Sluydts, V.

AU - Heng, S.

AU - Dourng, D.

AU - Eam, R.

AU - Chy, S.

AU - Khean, C.

AU - Loch, K.

AU - Ken, M.

AU - Lim, H.

AU - Siv, S.

AU - Tho, S.

AU - Masse-Navette, P.

AU - Gryseels, C.

AU - Uk, S.

AU - Van Roey, K.

AU - Peeters Grietens, K.

AU - Sokny, M.

AU - Thavrin, B.

AU - Chuor, C.M.

AU - Deubel, V.

AU - Durnez, L.

AU - Coosemans, M.

AU - Menard, D.

N1 - ITG-B4B; ITG-B5B; ITG-H16B; ITG-B18B; ITG-H19B; ITG-B24B; ITG-B25A; MULTI; DBM; U-ENTOM; DPH; U-MALAR; JIF; DOI; PDF; URL; Abstract; DSPACE56 FTX

PY - 2013

Y1 - 2013

N2 - BACKGROUND: To achieve the goal of malaria elimination in low transmission areas such as in Cambodia, new, inexpensive, high-throughput diagnostic tools for identifying very low parasite densities in asymptomatic carriers are required. This will enable a switch from passive to active malaria case detection in the field. METHODS: DNA extraction and real-time PCR assays were implemented in an "in-house" designed mobile laboratory allowing implementation of a robust, sensitive and rapid malaria diagnostic strategy in the field. This tool was employed in a survey organized in the context of the MalaResT project (NCT01663831). RESULTS: The real-time PCR screening and species identification assays were performed in the mobile laboratory between October and November 2012, in Rattanakiri Province, to screen approximately 5,000 individuals in less than four weeks and treat parasite carriers within 24--48 hours after sample collection. An average of 240 clinical samples (and 40 quality control samples) was tested every day, six/seven days per week. Some 97.7% of the results were available

AB - BACKGROUND: To achieve the goal of malaria elimination in low transmission areas such as in Cambodia, new, inexpensive, high-throughput diagnostic tools for identifying very low parasite densities in asymptomatic carriers are required. This will enable a switch from passive to active malaria case detection in the field. METHODS: DNA extraction and real-time PCR assays were implemented in an "in-house" designed mobile laboratory allowing implementation of a robust, sensitive and rapid malaria diagnostic strategy in the field. This tool was employed in a survey organized in the context of the MalaResT project (NCT01663831). RESULTS: The real-time PCR screening and species identification assays were performed in the mobile laboratory between October and November 2012, in Rattanakiri Province, to screen approximately 5,000 individuals in less than four weeks and treat parasite carriers within 24--48 hours after sample collection. An average of 240 clinical samples (and 40 quality control samples) was tested every day, six/seven days per week. Some 97.7% of the results were available

KW - Protozoal diseases

KW - Malaria

KW - Plasmodium falciparum

KW - Plasmodium vivax

KW - Plasmodium malariae

KW - Plasmodium ovale

KW - Vectors

KW - Mosquitoes

KW - Anopheles

KW - Diagnosis

KW - Detection

KW - Rapid diagnostic tests

KW - Real-time

KW - Polymerase chain reaction

KW - PCR

KW - Tools

KW - Development

KW - Case detection

KW - Carriers

KW - Mobile

KW - Cambodia

KW - Asia-Southeast

U2 - 10.1186/1475-2875-12-405

DO - 10.1186/1475-2875-12-405

M3 - A1: Web of Science-article

C2 - 24206649

SN - 1475-2875

VL - 12

SP - 1

EP - 12

JO - Malaria Journal

JF - Malaria Journal

IS - 405

ER -

An innovative tool for moving malaria PCR detection of parasite reservoir into the field

Abstract

Keywords

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