Antibody binding profile of purified and cell-bound CD26; designation of BT5/9 and TA5.9 to the CD26 cluster

I De Meester; S Scharpé; G Vanham; E Bosmans; H Heyligen; G Vanhoof; G Corte

doi:10.1016/S0171-2985(11)80494-8

Antibody binding profile of purified and cell-bound CD26; designation of BT5/9 and TA5.9 to the CD26 cluster

I De Meester, S Scharpé, G Vanham, E Bosmans, H Heyligen, G Vanhoof, G Corte

Virology

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

The CD26 activation antigen (Ag) which is expressed on a subpopulation of human T cells has been characterized as dipeptidyl peptidase IV (DPP IV, EC 3.4.14.5).In this paper, we describe the antibody binding profile of CD26/DPP IV, purified from human peripheral blood lymphocytes.The purified molecule binds to the anti-Ta1, anti-1F7 and anti-134-2C2 monoclonal antibodies (mAb), reported to react with cell-bound CD26 Ag.Among unclustered mAb recognizing T cell antigens, two, anti-BT5/9 and anti-TA5.9 were found to react with purified and cell-bound CD26 Ag.The classification of the BT5/9 Ag, the functional properties of the BT5/9+ T cell subset, as well as the in vivo effect of anti-BT5/9 mAb administration, are re-interpreted in the light of its specificity.
Applying the anti-TA5.9 mAb in three color FACS analyses, we demonstrated that CD26^+bright cells co-express CD45RO but not HLA-DR and CD38.

Original language	English
Journal	Immunobiology
Volume	188
Issue number	1-2
Pages (from-to)	145-158
Number of pages	8
DOIs	https://doi.org/10.1016/S0171-2985(11)80494-8
Publication status	Published - 1993

Keywords

B780-tropical-medicine
Virology
Immunology
Experimental
CD26

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10.1016/S0171-2985(11)80494-8

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title = "Antibody binding profile of purified and cell-bound CD26; designation of BT5/9 and TA5.9 to the CD26 cluster",

abstract = "The CD26 activation antigen (Ag) which is expressed on a subpopulation of human T cells has been characterized as dipeptidyl peptidase IV (DPP IV, EC 3.4.14.5).In this paper, we describe the antibody binding profile of CD26/DPP IV, purified from human peripheral blood lymphocytes.The purified molecule binds to the anti-Ta1, anti-1F7 and anti-134-2C2 monoclonal antibodies (mAb), reported to react with cell-bound CD26 Ag.Among unclustered mAb recognizing T cell antigens, two, anti-BT5/9 and anti-TA5.9 were found to react with purified and cell-bound CD26 Ag.The classification of the BT5/9 Ag, the functional properties of the BT5/9+ T cell subset, as well as the in vivo effect of anti-BT5/9 mAb administration, are re-interpreted in the light of its specificity.Applying the anti-TA5.9 mAb in three color FACS analyses, we demonstrated that CD26+bright cells co-express CD45RO but not HLA-DR and CD38.",

keywords = "B780-tropical-medicine, Virology, Immunology, Experimental, CD26",

author = "{De Meester}, I and S Scharp{\'e} and G Vanham and E Bosmans and H Heyligen and G Vanhoof and G Corte",

note = "This item is not available in the ITG Library",

year = "1993",

doi = "10.1016/S0171-2985(11)80494-8",

language = "English",

volume = "188",

pages = "145--158",

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T1 - Antibody binding profile of purified and cell-bound CD26; designation of BT5/9 and TA5.9 to the CD26 cluster

AU - De Meester, I

AU - Scharpé, S

AU - Vanham, G

AU - Bosmans, E

AU - Heyligen, H

AU - Vanhoof, G

AU - Corte, G

N1 - This item is not available in the ITG Library

PY - 1993

Y1 - 1993

N2 - The CD26 activation antigen (Ag) which is expressed on a subpopulation of human T cells has been characterized as dipeptidyl peptidase IV (DPP IV, EC 3.4.14.5).In this paper, we describe the antibody binding profile of CD26/DPP IV, purified from human peripheral blood lymphocytes.The purified molecule binds to the anti-Ta1, anti-1F7 and anti-134-2C2 monoclonal antibodies (mAb), reported to react with cell-bound CD26 Ag.Among unclustered mAb recognizing T cell antigens, two, anti-BT5/9 and anti-TA5.9 were found to react with purified and cell-bound CD26 Ag.The classification of the BT5/9 Ag, the functional properties of the BT5/9+ T cell subset, as well as the in vivo effect of anti-BT5/9 mAb administration, are re-interpreted in the light of its specificity.Applying the anti-TA5.9 mAb in three color FACS analyses, we demonstrated that CD26+bright cells co-express CD45RO but not HLA-DR and CD38.

AB - The CD26 activation antigen (Ag) which is expressed on a subpopulation of human T cells has been characterized as dipeptidyl peptidase IV (DPP IV, EC 3.4.14.5).In this paper, we describe the antibody binding profile of CD26/DPP IV, purified from human peripheral blood lymphocytes.The purified molecule binds to the anti-Ta1, anti-1F7 and anti-134-2C2 monoclonal antibodies (mAb), reported to react with cell-bound CD26 Ag.Among unclustered mAb recognizing T cell antigens, two, anti-BT5/9 and anti-TA5.9 were found to react with purified and cell-bound CD26 Ag.The classification of the BT5/9 Ag, the functional properties of the BT5/9+ T cell subset, as well as the in vivo effect of anti-BT5/9 mAb administration, are re-interpreted in the light of its specificity.Applying the anti-TA5.9 mAb in three color FACS analyses, we demonstrated that CD26+bright cells co-express CD45RO but not HLA-DR and CD38.

KW - B780-tropical-medicine

KW - Virology

KW - Immunology

KW - Experimental

KW - CD26

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:A1993LJ99500013

U2 - 10.1016/S0171-2985(11)80494-8

DO - 10.1016/S0171-2985(11)80494-8

M3 - A1: Web of Science-article

VL - 188

SP - 145

EP - 158

JO - Immunobiology

JF - Immunobiology

IS - 1-2

ER -

Antibody binding profile of purified and cell-bound CD26; designation of BT5/9 and TA5.9 to the CD26 cluster

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