Application of real-time PCR in Ghana, a Buruli ulcer-endemic country, confirms the presence of Mycobacterium ulcerans in the environment

K Vandelannoote; L Durnez; D Amissah; S Gryseels; A Dodoo; S Yeboah; P Addo; M Eddyani; H Leirs; A Ablordey; F Portaels

doi:10.1111/j.1574-6968.2010.01902.x

Application of real-time PCR in Ghana, a Buruli ulcer-endemic country, confirms the presence of Mycobacterium ulcerans in the environment

K Vandelannoote
, L Durnez
, D Amissah
, S Gryseels
, A Dodoo
, S Yeboah
, P Addo
, M Eddyani
, H Leirs
, A Ablordey
, F Portaels

Research output: Contribution to journal › A1: Peer-reviewed journal articles › peer-review

Abstract

This study reports the first successful application of real-time PCR for the detection of Mycobacterium ulcerans, the causative agent of Buruli ulcer (BU), in Ghana, a BU-endemic country. Environmental samples and organs of small mammals were analyzed. The real-time PCR assays confirmed the presence of M. ulcerans in a water sample collected in a BU-endemic village in the Ashanti Region.

Original language	English
Journal	FEMS Microbiology Letters
Volume	304
Issue number	2
Pages (from-to)	191-194
Number of pages	4
ISSN	0378-1097
DOIs	https://doi.org/10.1111/j.1574-6968.2010.01902.x
Publication status	Published - 2010

Keywords

B780-tropical-medicine
Bacterial diseases
Buruli ulcer
Mycobacterium ulcerans
Detection
Real-time
PCR
Water sampling
Environmental factors
Ghana
Africa-West

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http://lib.itg.be/pdf/itg/2010/2010fmle0191.pdf

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Vandelannoote, K., Durnez, L., Amissah, D., Gryseels, S., Dodoo, A., Yeboah, S., Addo, P., Eddyani, M., Leirs, H., Ablordey, A., & Portaels, F. (2010). Application of real-time PCR in Ghana, a Buruli ulcer-endemic country, confirms the presence of Mycobacterium ulcerans in the environment. FEMS Microbiology Letters, 304(2), 191-194. https://doi.org/10.1111/j.1574-6968.2010.01902.x

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title = "Application of real-time PCR in Ghana, a Buruli ulcer-endemic country, confirms the presence of Mycobacterium ulcerans in the environment",

abstract = "This study reports the first successful application of real-time PCR for the detection of Mycobacterium ulcerans, the causative agent of Buruli ulcer (BU), in Ghana, a BU-endemic country. Environmental samples and organs of small mammals were analyzed. The real-time PCR assays confirmed the presence of M. ulcerans in a water sample collected in a BU-endemic village in the Ashanti Region.",

keywords = "B780-tropical-medicine, Bacterial diseases, Buruli ulcer, Mycobacterium ulcerans, Detection, Real-time, PCR, Water sampling, Environmental factors, Ghana, Africa-West",

author = "K Vandelannoote and L Durnez and D Amissah and S Gryseels and A Dodoo and S Yeboah and P Addo and M Eddyani and H Leirs and A Ablordey and F Portaels",

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Vandelannoote, K, Durnez, L, Amissah, D, Gryseels, S, Dodoo, A, Yeboah, S, Addo, P, Eddyani, M, Leirs, H, Ablordey, A & Portaels, F 2010, 'Application of real-time PCR in Ghana, a Buruli ulcer-endemic country, confirms the presence of Mycobacterium ulcerans in the environment', FEMS Microbiology Letters, vol. 304, no. 2, pp. 191-194. https://doi.org/10.1111/j.1574-6968.2010.01902.x

TY - JOUR

T1 - Application of real-time PCR in Ghana, a Buruli ulcer-endemic country, confirms the presence of Mycobacterium ulcerans in the environment

AU - Vandelannoote, K

AU - Durnez, L

AU - Amissah, D

AU - Gryseels, S

AU - Dodoo, A

AU - Yeboah, S

AU - Addo, P

AU - Eddyani, M

AU - Leirs, H

AU - Ablordey, A

AU - Portaels, F

N1 - FTX abonnement

PY - 2010

Y1 - 2010

N2 - This study reports the first successful application of real-time PCR for the detection of Mycobacterium ulcerans, the causative agent of Buruli ulcer (BU), in Ghana, a BU-endemic country. Environmental samples and organs of small mammals were analyzed. The real-time PCR assays confirmed the presence of M. ulcerans in a water sample collected in a BU-endemic village in the Ashanti Region.

AB - This study reports the first successful application of real-time PCR for the detection of Mycobacterium ulcerans, the causative agent of Buruli ulcer (BU), in Ghana, a BU-endemic country. Environmental samples and organs of small mammals were analyzed. The real-time PCR assays confirmed the presence of M. ulcerans in a water sample collected in a BU-endemic village in the Ashanti Region.

KW - B780-tropical-medicine

KW - Bacterial diseases

KW - Buruli ulcer

KW - Mycobacterium ulcerans

KW - Detection

KW - Real-time

KW - PCR

KW - Water sampling

KW - Environmental factors

KW - Ghana

KW - Africa-West

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000274482300012

U2 - 10.1111/j.1574-6968.2010.01902.x

DO - 10.1111/j.1574-6968.2010.01902.x

M3 - A1: Peer-reviewed journal articles

SN - 0378-1097

VL - 304

SP - 191

EP - 194

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -

Application of real-time PCR in Ghana, a Buruli ulcer-endemic country, confirms the presence of Mycobacterium ulcerans in the environment

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Keywords

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