Bayesian modelling to estimate the test characteristics of coprology, coproantigen ELISA and a novel real-time PCR for the diagnosis of taeniasis

N. Praet, J.J. Verweij, K.E. Mwape, I.K. Phiri, J.B. Muma, G. Zulu, L. van Lieshout, R. Rodriguez-Hidalgo, W. Benitez-Ortiz, P. Dorny, S. Gabriël

Research output: Contribution to journalA1: Web of Science-article

Abstract

OBJECTIVE: To estimate and compare the performances of coprology, copro-Ag ELISA and real-time polymerase chain reaction assay (copro-PCR) for detection of Taenia solium tapeworm carriers. METHODS: The three diagnostic tests were applied on 817 stool samples collected in two Zambian communities where taeniasis is endemic. A Bayesian approach was used to allow estimation of the test characteristics. Two (0.2%; 95% Confidence Interval (CI): 0-0.8), 67 (8.2%; 95% CI: 6.4-10.3) and 10 (1.2%; 95% CI: 0.5-2.2) samples were positive using coprology, copro-Ag ELISA and copro-PCR, respectively. RESULTS: Specificities of 99.9%, 92.0% and 99.0% were determined for coprology, copro-Ag ELISA and copro-PCR, respectively. Sensitivities of 52.5%, 84.5% and 82.7% were determined for coprology, copro-Ag ELISA and copro-PCR, respectively. CONCLUSIONS: We urge for additional studies exploring possible cross-reactions of the copro-Ag ELISA and for the use of more sensitive tests, such as copro-PCR, for the detection of tapeworm carriers, which is a key factor in controlling the parasite in endemic areas.
Original languageEnglish
JournalTropical Medicine and International Health
Volume18
Issue number5
Pages (from-to)608-614
Number of pages7
ISSN1360-2276
DOIs
Publication statusPublished - 2013

Keywords

  • Helminthic diseases
  • Taeniasis
  • Taenia solium
  • Taenia saginata
  • Diagnosis
  • Evaluation
  • Performance
  • Comparison
  • Bayes theorem
  • Modeling
  • Coprology
  • ELISA
  • Real-time
  • Polymerase chain reaction
  • PCR
  • Specificity
  • Sensitivity
  • Probabilities
  • Laboratory techniques and procedures

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