Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes

M Itkin; U Heinig; O Tzfadia; A J Bhide; B Shinde; P D Cardenas; S E Bocobza; T Unger; S Malitsky; R Finkers; Y Tikunov; A Bovy; Y Chikate; P Singh; I Rogachev; J Beekwilder; A P Giri; A Aharoni

doi:10.1126/science.1240230

Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes

M Itkin, U Heinig, O Tzfadia, A J Bhide, B Shinde, P D Cardenas, S E Bocobza, T Unger, S Malitsky, R Finkers, Y Tikunov, A Bovy, Y Chikate, P Singh, I Rogachev, J Beekwilder, A P Giri, A Aharoni

Mycobacteriology

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Steroidal glycoalkaloids (SGAs) such as α-solanine found in solanaceous food plants--as, for example, potato--are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.

Original language	English
Journal	Science
Volume	341
Issue number	6142
Pages (from-to)	175-179
Number of pages	5
ISSN	0036-8075
DOIs	https://doi.org/10.1126/science.1240230
Publication status	Published - 2013

Keywords

Crops, Agricultural/genetics
Gene Expression Profiling
Gene Expression Regulation, Plant
Gene Silencing
Genes, Plant
Lycopersicon esculentum/genetics
Multigene Family
Nutritive Value/genetics
Solanaceous Alkaloids/biosynthesis
Solanum tuberosum/genetics

Access to Document

10.1126/science.1240230

Cite this

Itkin, M., Heinig, U., Tzfadia, O., Bhide, A. J., Shinde, B., Cardenas, P. D., Bocobza, S. E., Unger, T., Malitsky, S., Finkers, R., Tikunov, Y., Bovy, A., Chikate, Y., Singh, P., Rogachev, I., Beekwilder, J., Giri, A. P., & Aharoni, A. (2013). Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes. Science, 341(6142), 175-179. https://doi.org/10.1126/science.1240230

@article{cb119f6d3d1d486bac31638492416537,

title = "Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes",

abstract = "Steroidal glycoalkaloids (SGAs) such as α-solanine found in solanaceous food plants--as, for example, potato--are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.",

keywords = "Crops, Agricultural/genetics, Gene Expression Profiling, Gene Expression Regulation, Plant, Gene Silencing, Genes, Plant, Lycopersicon esculentum/genetics, Multigene Family, Nutritive Value/genetics, Solanaceous Alkaloids/biosynthesis, Solanum tuberosum/genetics",

author = "M Itkin and U Heinig and O Tzfadia and Bhide, {A J} and B Shinde and Cardenas, {P D} and Bocobza, {S E} and T Unger and S Malitsky and R Finkers and Y Tikunov and A Bovy and Y Chikate and P Singh and I Rogachev and J Beekwilder and Giri, {A P} and A Aharoni",

year = "2013",

doi = "10.1126/science.1240230",

language = "English",

volume = "341",

pages = "175--179",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "6142",

}

Itkin, M, Heinig, U, Tzfadia, O, Bhide, AJ, Shinde, B, Cardenas, PD, Bocobza, SE, Unger, T, Malitsky, S, Finkers, R, Tikunov, Y, Bovy, A, Chikate, Y, Singh, P, Rogachev, I, Beekwilder, J, Giri, AP & Aharoni, A 2013, 'Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes', Science, vol. 341, no. 6142, pp. 175-179. https://doi.org/10.1126/science.1240230

TY - JOUR

T1 - Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes

AU - Itkin, M

AU - Heinig, U

AU - Tzfadia, O

AU - Bhide, A J

AU - Shinde, B

AU - Cardenas, P D

AU - Bocobza, S E

AU - Unger, T

AU - Malitsky, S

AU - Finkers, R

AU - Tikunov, Y

AU - Bovy, A

AU - Chikate, Y

AU - Singh, P

AU - Rogachev, I

AU - Beekwilder, J

AU - Giri, A P

AU - Aharoni, A

PY - 2013

Y1 - 2013

N2 - Steroidal glycoalkaloids (SGAs) such as α-solanine found in solanaceous food plants--as, for example, potato--are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.

AB - Steroidal glycoalkaloids (SGAs) such as α-solanine found in solanaceous food plants--as, for example, potato--are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.

KW - Crops, Agricultural/genetics

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Plant

KW - Gene Silencing

KW - Genes, Plant

KW - Lycopersicon esculentum/genetics

KW - Multigene Family

KW - Nutritive Value/genetics

KW - Solanaceous Alkaloids/biosynthesis

KW - Solanum tuberosum/genetics

U2 - 10.1126/science.1240230

DO - 10.1126/science.1240230

M3 - A1: Web of Science-article

C2 - 23788733

SN - 0036-8075

VL - 341

SP - 175

EP - 179

JO - Science

JF - Science

IS - 6142

ER -

Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes

Abstract

Keywords

Access to Document

Fingerprint

Cite this