Candidate Treponema pallidum biomarkers uncovered in urine from individuals with syphilis using mass spectrometry

Kara K Osbak, Geert A Van Raemdonck, Martin Dom, Caroline E Cameron, Conor J Meehan, Dieter Deforce, Xaveer Van Ostade, Chris R Kenyon, Maarten Dhaenens

Research output: Contribution to journalA1: Web of Science-article

Abstract

AIM: A diagnostic test that could detect Treponema pallidum antigens in urine would facilitate the prompt diagnosis of syphilis.

MATERIALS & METHODS: Urine from 54 individuals with various clinical stages of syphilis and 6 controls were pooled according to disease stage and interrogated with complementary mass spectrometry techniques to uncover potential syphilis biomarkers.

RESULTS & CONCLUSION: In total, 26 unique peptides were uncovered corresponding to four unique T. pallidum proteins that have low genetic sequence similarity to other prokaryotes and human proteins. This is the first account of direct T. pallidum protein detection in human clinical samples using mass spectrometry. The implications of these findings for future diagnostic test development is discussed. Data are available via ProteomeXchange with identifier PXD009707.

Original languageEnglish
JournalFuture Microbiology
Volume13
Pages (from-to)1497-1510
Number of pages14
ISSN1746-0913
DOIs
Publication statusPublished - 2018

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