Candidate Treponema pallidum biomarkers uncovered in urine from individuals with syphilis using mass spectrometry

Kara K Osbak; Geert A Van Raemdonck; Martin Dom; Caroline E Cameron; Conor J Meehan; Dieter Deforce; Xaveer Van Ostade; Chris R Kenyon; Maarten Dhaenens

doi:10.2217/fmb-2018-0182

Candidate Treponema pallidum biomarkers uncovered in urine from individuals with syphilis using mass spectrometry

Kara K Osbak, Geert A Van Raemdonck, Martin Dom, Caroline E Cameron, Conor J Meehan, Dieter Deforce, Xaveer Van Ostade, Chris R Kenyon, Maarten Dhaenens

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

AIM: A diagnostic test that could detect Treponema pallidum antigens in urine would facilitate the prompt diagnosis of syphilis.

MATERIALS & METHODS: Urine from 54 individuals with various clinical stages of syphilis and 6 controls were pooled according to disease stage and interrogated with complementary mass spectrometry techniques to uncover potential syphilis biomarkers.

RESULTS & CONCLUSION: In total, 26 unique peptides were uncovered corresponding to four unique T. pallidum proteins that have low genetic sequence similarity to other prokaryotes and human proteins. This is the first account of direct T. pallidum protein detection in human clinical samples using mass spectrometry. The implications of these findings for future diagnostic test development is discussed. Data are available via ProteomeXchange with identifier PXD009707.

Original language	English
Journal	Future Microbiology
Volume	13
Pages (from-to)	1497-1510
Number of pages	14
ISSN	1746-0913
DOIs	https://doi.org/10.2217/fmb-2018-0182
Publication status	Published - 2018

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title = "Candidate Treponema pallidum biomarkers uncovered in urine from individuals with syphilis using mass spectrometry",

abstract = "AIM: A diagnostic test that could detect Treponema pallidum antigens in urine would facilitate the prompt diagnosis of syphilis.MATERIALS & METHODS: Urine from 54 individuals with various clinical stages of syphilis and 6 controls were pooled according to disease stage and interrogated with complementary mass spectrometry techniques to uncover potential syphilis biomarkers.RESULTS & CONCLUSION: In total, 26 unique peptides were uncovered corresponding to four unique T. pallidum proteins that have low genetic sequence similarity to other prokaryotes and human proteins. This is the first account of direct T. pallidum protein detection in human clinical samples using mass spectrometry. The implications of these findings for future diagnostic test development is discussed. Data are available via ProteomeXchange with identifier PXD009707.",

author = "Osbak, {Kara K} and {Van Raemdonck}, {Geert A} and Martin Dom and Cameron, {Caroline E} and Meehan, {Conor J} and Dieter Deforce and Ostade, {Xaveer Van} and Kenyon, {Chris R} and Maarten Dhaenens",

note = "NPP; CUNIT2",

year = "2018",

doi = "10.2217/fmb-2018-0182",

language = "English",

volume = "13",

pages = "1497--1510",

journal = "Future Microbiology",

issn = "1746-0913",

publisher = "Future Medicine Ltd.",

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TY - JOUR

T1 - Candidate Treponema pallidum biomarkers uncovered in urine from individuals with syphilis using mass spectrometry

AU - Osbak, Kara K

AU - Van Raemdonck, Geert A

AU - Dom, Martin

AU - Cameron, Caroline E

AU - Meehan, Conor J

AU - Deforce, Dieter

AU - Ostade, Xaveer Van

AU - Kenyon, Chris R

AU - Dhaenens, Maarten

N1 - NPP; CUNIT2

PY - 2018

Y1 - 2018

N2 - AIM: A diagnostic test that could detect Treponema pallidum antigens in urine would facilitate the prompt diagnosis of syphilis.MATERIALS & METHODS: Urine from 54 individuals with various clinical stages of syphilis and 6 controls were pooled according to disease stage and interrogated with complementary mass spectrometry techniques to uncover potential syphilis biomarkers.RESULTS & CONCLUSION: In total, 26 unique peptides were uncovered corresponding to four unique T. pallidum proteins that have low genetic sequence similarity to other prokaryotes and human proteins. This is the first account of direct T. pallidum protein detection in human clinical samples using mass spectrometry. The implications of these findings for future diagnostic test development is discussed. Data are available via ProteomeXchange with identifier PXD009707.

AB - AIM: A diagnostic test that could detect Treponema pallidum antigens in urine would facilitate the prompt diagnosis of syphilis.MATERIALS & METHODS: Urine from 54 individuals with various clinical stages of syphilis and 6 controls were pooled according to disease stage and interrogated with complementary mass spectrometry techniques to uncover potential syphilis biomarkers.RESULTS & CONCLUSION: In total, 26 unique peptides were uncovered corresponding to four unique T. pallidum proteins that have low genetic sequence similarity to other prokaryotes and human proteins. This is the first account of direct T. pallidum protein detection in human clinical samples using mass spectrometry. The implications of these findings for future diagnostic test development is discussed. Data are available via ProteomeXchange with identifier PXD009707.

U2 - 10.2217/fmb-2018-0182

DO - 10.2217/fmb-2018-0182

M3 - A1: Web of Science-article

C2 - 30311792

SN - 1746-0913

VL - 13

SP - 1497

EP - 1510

JO - Future Microbiology

JF - Future Microbiology

ER -

Candidate Treponema pallidum biomarkers uncovered in urine from individuals with syphilis using mass spectrometry

Abstract

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