CD32(+)CD4(+) memory T cells are enriched for total HIV-1 DNA in tissues from humanized mice

Philipp Adams, Virginie Fievez, Rafaela Schober, Mathieu Amand, Gilles Iserentant, Sofie Rutsaert, Geraldine Dessilly, Guido Vanham, Fanny Hedin, Antonio Cosma, Michel Moutschen, Linos Vandekerckhove, Carole Seguin-Devaux

Research output: Contribution to journalA1: Web of Science-article

Abstract

CD32 has raised conflicting results as a putative marker of the HIV-1 reservoir. We measured CD32 expression in tissues from viremic and virally suppressed humanized mice treated relatively early or late after HIV-1 infection with combined antiretroviral therapy. CD32 was expressed in a small fraction of the memory CD4(+) T-cell subsets from different tissues in viremic and aviremic mice, regardless of treatment initiation time. CD32(+) memory CD4(+) T cells were enriched in cell associated (CA) HIV-1 DNA but not in CA HIV-1 RNA as compared to the CD32(-) CD4(+) fraction. Using multidimensional reduction analysis, several memory CD4(+)CD32(+) T-cell clusters were identified expressing HLA-DR, TIGIT, or PD-1. Importantly, although tissue-resident CD32(+)CD4(+) memory cells were enriched with translation-competent reservoirs, most of it was detected in memory CD32-CD4(+) T cells. Our findings support that CD32 labels highly activated/exhausted memory CD4(+) T-cell subsets that contain only a small proportion of the translation-competent reservoir.

Original languageEnglish
Article number101881
JournalIscience
Volume24
Issue number1
Number of pages33
DOIs
Publication statusPublished - 2021

Keywords

  • ANTIRETROVIRAL THERAPY
  • LATENT RESERVOIR
  • INFECTED INDIVIDUALS
  • IMMUNE ACTIVATION
  • ANTIVIRAL THERAPY
  • CD4(+)
  • REPLICATION
  • PERSISTENCE
  • EXPRESSION
  • BLOOD

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