Abstract
CD32 has raised conflicting results as a putative marker of the HIV-1 reservoir. We measured CD32 expression in tissues from viremic and virally suppressed humanized mice treated relatively early or late after HIV-1 infection with combined antiretroviral therapy. CD32 was expressed in a small fraction of the memory CD4(+) T-cell subsets from different tissues in viremic and aviremic mice, regardless of treatment initiation time. CD32(+) memory CD4(+) T cells were enriched in cell associated (CA) HIV-1 DNA but not in CA HIV-1 RNA as compared to the CD32(-) CD4(+) fraction. Using multidimensional reduction analysis, several memory CD4(+)CD32(+) T-cell clusters were identified expressing HLA-DR, TIGIT, or PD-1. Importantly, although tissue-resident CD32(+)CD4(+) memory cells were enriched with translation-competent reservoirs, most of it was detected in memory CD32-CD4(+) T cells. Our findings support that CD32 labels highly activated/exhausted memory CD4(+) T-cell subsets that contain only a small proportion of the translation-competent reservoir.
Original language | English |
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Article number | 101881 |
Journal | Iscience |
Volume | 24 |
Issue number | 1 |
Number of pages | 33 |
DOIs | |
Publication status | Published - 2021 |
Keywords
- ANTIRETROVIRAL THERAPY
- LATENT RESERVOIR
- INFECTED INDIVIDUALS
- IMMUNE ACTIVATION
- ANTIVIRAL THERAPY
- CD4(+)
- REPLICATION
- PERSISTENCE
- EXPRESSION
- BLOOD