Characterization of Trypanosoma brucei gambiense variant surface glycoprotein LiTat 1.5

L. Van Nieuwenhove; S. Rogé; V. Lejon; Y. Guisez; Philippe Büscher

doi:10.4238/2012.May.9.5

Characterization of Trypanosoma brucei gambiense variant surface glycoprotein LiTat 1.5

L. Van Nieuwenhove, S. Rogé, V. Lejon, Y. Guisez, Philippe Büscher

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

At present, all available diagnostic antibody detection tests for Trypanosoma brucei gambiense human African trypanosomiasis are based on predominant variant surface glycoproteins (VSGs), such as VSG LiTat 1.5. During investigations aiming at replacement of the native VSGs by recombinant proteins or synthetic peptides, the sequence of VSG LiTat 1.5 was derived from cDNA and direct N-terminal amino acid sequencing. Characterization of the VSG based on cysteine distribution in the amino acid sequence revealed an unusual cysteine pattern identical to that of VSG Kinu 1 of T. b. brucei. Even though both VSGs lack the third of four conserved cysteines typical for type A N-terminal domains, they can be classified as type A.

Original language	English
Journal	Genetics and Molecular Research
Volume	11
Issue number	2
Pages (from-to)	1260-1265
Number of pages	6
ISSN	1676-5680
DOIs	https://doi.org/10.4238/2012.May.9.5
Publication status	Published - 2012

Keywords

Protozoal diseases
Trypanosomiasis-African
Sleeping sickness
Trypanosoma brucei gambiense
Vectors
Tsetse flies
Glossina
Characterization
Variants
Surface glycoproteins
VSG
Amino acid sequences
LiTat 1.5
Detection
Card agglutination test
CATT
Laboratory techniques and procedures

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@article{0cd42215698b4e97be0154c0ac044414,

title = "Characterization of Trypanosoma brucei gambiense variant surface glycoprotein LiTat 1.5",

abstract = "At present, all available diagnostic antibody detection tests for Trypanosoma brucei gambiense human African trypanosomiasis are based on predominant variant surface glycoproteins (VSGs), such as VSG LiTat 1.5. During investigations aiming at replacement of the native VSGs by recombinant proteins or synthetic peptides, the sequence of VSG LiTat 1.5 was derived from cDNA and direct N-terminal amino acid sequencing. Characterization of the VSG based on cysteine distribution in the amino acid sequence revealed an unusual cysteine pattern identical to that of VSG Kinu 1 of T. b. brucei. Even though both VSGs lack the third of four conserved cysteines typical for type A N-terminal domains, they can be classified as type A.",

keywords = "Protozoal diseases, Trypanosomiasis-African, Sleeping sickness, Trypanosoma brucei gambiense, Vectors, Tsetse flies, Glossina, Characterization, Variants, Surface glycoproteins, VSG, Amino acid sequences, LiTat 1.5, Detection, Card agglutination test, CATT, Laboratory techniques and procedures",

author = "{Van Nieuwenhove}, L. and S. Rog{\'e} and V. Lejon and Y. Guisez and Philippe B{\"u}scher",

note = "PPU; ITG-B1B; ITG-B2B; ITG-B3A; ITG-BLA; DBM; U-PARDIA; JIF; DOI; PDF; DSPACE",

year = "2012",

doi = "10.4238/2012.May.9.5",

language = "English",

volume = "11",

pages = "1260--1265",

journal = "Genetics and Molecular Research",

issn = "1676-5680",

publisher = "Fundacao de Pesquisas Cientificas de Ribeirao Preto",

number = "2",

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TY - JOUR

T1 - Characterization of Trypanosoma brucei gambiense variant surface glycoprotein LiTat 1.5

AU - Van Nieuwenhove, L.

AU - Rogé, S.

AU - Lejon, V.

AU - Guisez, Y.

AU - Büscher, Philippe

N1 - PPU; ITG-B1B; ITG-B2B; ITG-B3A; ITG-BLA; DBM; U-PARDIA; JIF; DOI; PDF; DSPACE

PY - 2012

Y1 - 2012

N2 - At present, all available diagnostic antibody detection tests for Trypanosoma brucei gambiense human African trypanosomiasis are based on predominant variant surface glycoproteins (VSGs), such as VSG LiTat 1.5. During investigations aiming at replacement of the native VSGs by recombinant proteins or synthetic peptides, the sequence of VSG LiTat 1.5 was derived from cDNA and direct N-terminal amino acid sequencing. Characterization of the VSG based on cysteine distribution in the amino acid sequence revealed an unusual cysteine pattern identical to that of VSG Kinu 1 of T. b. brucei. Even though both VSGs lack the third of four conserved cysteines typical for type A N-terminal domains, they can be classified as type A.

AB - At present, all available diagnostic antibody detection tests for Trypanosoma brucei gambiense human African trypanosomiasis are based on predominant variant surface glycoproteins (VSGs), such as VSG LiTat 1.5. During investigations aiming at replacement of the native VSGs by recombinant proteins or synthetic peptides, the sequence of VSG LiTat 1.5 was derived from cDNA and direct N-terminal amino acid sequencing. Characterization of the VSG based on cysteine distribution in the amino acid sequence revealed an unusual cysteine pattern identical to that of VSG Kinu 1 of T. b. brucei. Even though both VSGs lack the third of four conserved cysteines typical for type A N-terminal domains, they can be classified as type A.

KW - Protozoal diseases

KW - Trypanosomiasis-African

KW - Sleeping sickness

KW - Trypanosoma brucei gambiense

KW - Vectors

KW - Tsetse flies

KW - Glossina

KW - Characterization

KW - Variants

KW - Surface glycoproteins

KW - VSG

KW - Amino acid sequences

KW - LiTat 1.5

KW - Detection

KW - Card agglutination test

KW - CATT

KW - Laboratory techniques and procedures

U2 - 10.4238/2012.May.9.5

DO - 10.4238/2012.May.9.5

M3 - A1: Web of Science-article

C2 - 22614354

SN - 1676-5680

VL - 11

SP - 1260

EP - 1265

JO - Genetics and Molecular Research

JF - Genetics and Molecular Research

IS - 2

ER -

Characterization of Trypanosoma brucei gambiense variant surface glycoprotein LiTat 1.5

Abstract

Keywords

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