Characterization of Trypanosoma brucei gambiense variant surface glycoprotein LiTat 1.5

L. Van Nieuwenhove, S. Rogé, V. Lejon, Y. Guisez, Philippe Büscher

Research output: Contribution to journalA1: Web of Science-article

Abstract

At present, all available diagnostic antibody detection tests for Trypanosoma brucei gambiense human African trypanosomiasis are based on predominant variant surface glycoproteins (VSGs), such as VSG LiTat 1.5. During investigations aiming at replacement of the native VSGs by recombinant proteins or synthetic peptides, the sequence of VSG LiTat 1.5 was derived from cDNA and direct N-terminal amino acid sequencing. Characterization of the VSG based on cysteine distribution in the amino acid sequence revealed an unusual cysteine pattern identical to that of VSG Kinu 1 of T. b. brucei. Even though both VSGs lack the third of four conserved cysteines typical for type A N-terminal domains, they can be classified as type A.
Original languageEnglish
JournalGenetics and Molecular Research
Volume11
Issue number2
Pages (from-to)1260-1265
Number of pages6
ISSN1676-5680
DOIs
Publication statusPublished - 2012

Keywords

  • Protozoal diseases
  • Trypanosomiasis-African
  • Sleeping sickness
  • Trypanosoma brucei gambiense
  • Vectors
  • Tsetse flies
  • Glossina
  • Characterization
  • Variants
  • Surface glycoproteins
  • VSG
  • Amino acid sequences
  • LiTat 1.5
  • Detection
  • Card agglutination test
  • CATT
  • Laboratory techniques and procedures

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