Comparative diagnosis of parasitological, serological, and molecular tests in dourine-suspected horses

FR Gari, H Ashenafi, A Tola, BM Goddeeris, F Claes

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    Study on comparative sensitivity of parasitological, serological, and molecular tests on 237 horses originating from two dourine-suspected districts of Arsi-Bale highlands of Ethiopia was conducted to determine the prevalence of the disease and degree of agreement of the diagnostic tests. Accordingly, the prevalence of the disease was found to be 4.6%, 36.7%, and 47.6% by parasitological Woo test, RoTat 1.2 and 18S PCR tests, respectively. The seroprevalence of the disease was 27.6% in CATT/Trypanosoma evansi test. In Ethiopia, it was for the first time that trypanosomes from dourine suspected horses were demonstrated in 4.6% of the animals using Woo test. The findings of the present study disclosed that dourine is highly prevalent and one of the major diseases of horses in the area. There was no statistically significant difference (P > 0.05) in prevalence of the disease between districts, sexes, and age groups of the animals. However, there was a statistically significant difference (P < 0.05) in the prevalence of the disease between emaciated and animals with good body condition. Assessment of the degree of agreement of the diagnostic tests employed revealed low to fair [Formula: see text] with significantly higher sensitivity by PCR than other tests
    Original languageEnglish
    JournalTropical Animal Health and Production
    Volume42
    Issue number8
    Pages (from-to)1649-1654
    Number of pages6
    ISSN0049-4747
    DOIs
    Publication statusPublished - 2010

    Keywords

    • B780-tropical-medicine
    • Animal diseases
    • Dourine
    • Trypanosoma equiperdum
    • Vectors
    • Glossina
    • Horses
    • Prevalence
    • Comparison
    • Rapid diagnostic tests
    • Parasitology
    • Serology
    • Molecular diagnostic techniques
    • CATT
    • RoTat 1.2
    • PCR
    • Ethiopia
    • Africa-East

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