Comparative genomics of Burkholderia singularis sp. nov., a low G+C content, free-living bacterium that defies taxonomic dissection of the genus Burkholderia

Peter Vandamme, Charlotte Peeters, Birgit De Smet, Erin P Price, Derek S Sarovich, Deborah A Henry, Trevor J Hird, James E A Zlosnik, Mark Mayo, Jeffrey Warner, Anthony Baker, Bart J Currie, Aurélien Carlier

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Abstract

Four Burkholderia pseudomallei-like isolates of human clinical origin were examined by a polyphasic taxonomic approach that included comparative whole genome analyses. The results demonstrated that these isolates represent a rare and unusual, novelBurkholderiaspecies for which we propose the nameB. singularis.The type strain is LMG 28154T(=CCUG 65685T). Its genome sequence has an average mol% G+C content of 64.34%, which is considerably lower than that of otherBurkholderiaspecies. The reduced G+C content of strain LMG 28154Twas characterized by a genome wide AT bias that was not due to reduced GC-biased gene conversion or reductive genome evolution, but might have been caused by an altered DNA base excision repair pathway.B. singulariscan be differentiated from otherBurkholderiaspecies by multilocus sequence analysis, MALDI-TOF mass spectrometry and a distinctive biochemical profile that includes the absence of nitrate reduction, a mucoid appearance on Columbia sheep blood agar, and a slowly positive oxidase reaction. Comparisons with publicly available whole genome sequences demonstrated that strain TSV85, an Australian water isolate, also represents the same species and therefore, to date,B. singularishas been recovered from human or environmental samples on three continents.

Original languageEnglish
Article number1679
JournalFrontiers in Microbiology
Volume8
Number of pages14
ISSN1664-302X
DOIs
Publication statusPublished - 2017
Externally publishedYes

Keywords

  • Journal Article

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