Comparison of chromosome and isoenzyme polymorphism in geographical populations of Leishmania (Viannia) peruviana

JC Dujardin, AL Bañuls, JP Dujardin, J Arevalo, M Tibayrenc, D Le Ray

Research output: Contribution to journalA1: Web of Science-articlepeer-review

Abstract

Five chromosomes and 17 isoenzyme loci were analysed in 4 allopatric populations of Leishmania (Viannia) peruviana, and molecular distances calculated with 2 estimators, Chromosomal Size Difference Index and Jaccard Distance. Chromosome and isoenzyme data were in overall concordance: 13/30 isolates clustered similarly on the dendrograms constructed from the different estimators, and a significant correlation (P<0·001) was observed between the molecular distances calculated from the two sets of characters. This indicates an evolutionary association between chromosomal size polymorphism and isoenzymes. Chromosomes have a faster molecular clock than isoenzymes; twice as many genotypes were identified by chromosome analysis and significant size differences (for a total of up to 500 kb for 5 chromosomes together) were observed within a given zymodeme. Chromosomes most likely represent better indicators of genetic drift than isoenzymes, as suggested by the higher correlation between both estimators of chromosomal size-polymorphism and eco-geography. Some chromosomes might present an adaptive response to environmental variation.
Original languageEnglish
JournalParasitology
Volume117
Pages (from-to)547-554
Number of pages8
ISSN0031-1820
DOIs
Publication statusPublished - 1998

Keywords

  • B780-tropical-medicine
  • Protozoology
  • Leishmania peruviana
  • Genetics
  • Polymorphism
  • Chromosomes
  • Isoenzymes

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