Comparison of four PCR methods for efficient detection of Trypanosoma cruzi in routine diagnostics

Peter Seiringer; Michael Pritsch; María Flores-Chavez; Edoardo Marchisio; Kerstin Helfrich; Carolin Mengele; Stefan Hohnerlein; Gisela Bretzel; Thomas Löscher; Michael Hoelscher; Nicole Berens-Riha

doi:10.1016/j.diagmicrobio.2017.04.003

Comparison of four PCR methods for efficient detection of Trypanosoma cruzi in routine diagnostics

Peter Seiringer, Michael Pritsch, María Flores-Chavez, Edoardo Marchisio, Kerstin Helfrich, Carolin Mengele, Stefan Hohnerlein, Gisela Bretzel, Thomas Löscher, Michael Hoelscher, Nicole Berens-Riha

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Due to increased migration, Chagas disease has become an international health problem. Reliable diagnosis of chronically infected people is crucial for prevention of non-vectorial transmission as well as treatment. This study compared four distinct PCR methods for detection of Trypanosoma cruzi DNA for the use in well-equipped routine diagnostic laboratories. DNA was extracted of T. cruzi-positive and negative patients' blood samples and cultured T. cruzi, T. rangeli as well as Leishmania spp. One conventional and two real-time PCR methods targeting a repetitive Sat-DNA sequence as well as one conventional PCR method targeting the variable region of the kDNA minicircle were compared for sensitivity, intra- and interassay precision, limit of detection, specificity and cross-reactivity. Considering the performance, costs and ease of use, an algorithm for PCR-diagnosis of patients with a positive serology for T. cruzi antibodies was developed.

Original language	English
Journal	Diagnostic Microbiology and Infectious Disease
Volume	88
Issue number	3
Pages (from-to)	225-232
Number of pages	8
ISSN	0732-8893
DOIs	https://doi.org/10.1016/j.diagmicrobio.2017.04.003
Publication status	Published - 2017

Keywords

Adolescent
Adult
Blood/parasitology
Chagas Disease/diagnosis
Child, Preschool
Female
Humans
Male
Middle Aged
Molecular Diagnostic Techniques/methods
Polymerase Chain Reaction/methods
Sensitivity and Specificity
Trypanosoma cruzi/genetics
Young Adult

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10.1016/j.diagmicrobio.2017.04.003

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Seiringer, P., Pritsch, M., Flores-Chavez, M., Marchisio, E., Helfrich, K., Mengele, C., Hohnerlein, S., Bretzel, G., Löscher, T., Hoelscher, M., & Berens-Riha, N. (2017). Comparison of four PCR methods for efficient detection of Trypanosoma cruzi in routine diagnostics. Diagnostic Microbiology and Infectious Disease, 88(3), 225-232. https://doi.org/10.1016/j.diagmicrobio.2017.04.003

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title = "Comparison of four PCR methods for efficient detection of Trypanosoma cruzi in routine diagnostics",

abstract = "Due to increased migration, Chagas disease has become an international health problem. Reliable diagnosis of chronically infected people is crucial for prevention of non-vectorial transmission as well as treatment. This study compared four distinct PCR methods for detection of Trypanosoma cruzi DNA for the use in well-equipped routine diagnostic laboratories. DNA was extracted of T. cruzi-positive and negative patients' blood samples and cultured T. cruzi, T. rangeli as well as Leishmania spp. One conventional and two real-time PCR methods targeting a repetitive Sat-DNA sequence as well as one conventional PCR method targeting the variable region of the kDNA minicircle were compared for sensitivity, intra- and interassay precision, limit of detection, specificity and cross-reactivity. Considering the performance, costs and ease of use, an algorithm for PCR-diagnosis of patients with a positive serology for T. cruzi antibodies was developed.",

keywords = "Adolescent, Adult, Blood/parasitology, Chagas Disease/diagnosis, Child, Preschool, Female, Humans, Male, Middle Aged, Molecular Diagnostic Techniques/methods, Polymerase Chain Reaction/methods, Sensitivity and Specificity, Trypanosoma cruzi/genetics, Young Adult",

author = "Peter Seiringer and Michael Pritsch and Mar{\'i}a Flores-Chavez and Edoardo Marchisio and Kerstin Helfrich and Carolin Mengele and Stefan Hohnerlein and Gisela Bretzel and Thomas L{\"o}scher and Michael Hoelscher and Nicole Berens-Riha",

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doi = "10.1016/j.diagmicrobio.2017.04.003",

language = "English",

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Seiringer, P, Pritsch, M, Flores-Chavez, M, Marchisio, E, Helfrich, K, Mengele, C, Hohnerlein, S, Bretzel, G, Löscher, T, Hoelscher, M & Berens-Riha, N 2017, 'Comparison of four PCR methods for efficient detection of Trypanosoma cruzi in routine diagnostics', Diagnostic Microbiology and Infectious Disease, vol. 88, no. 3, pp. 225-232. https://doi.org/10.1016/j.diagmicrobio.2017.04.003

TY - JOUR

T1 - Comparison of four PCR methods for efficient detection of Trypanosoma cruzi in routine diagnostics

AU - Seiringer, Peter

AU - Pritsch, Michael

AU - Flores-Chavez, María

AU - Marchisio, Edoardo

AU - Helfrich, Kerstin

AU - Mengele, Carolin

AU - Hohnerlein, Stefan

AU - Bretzel, Gisela

AU - Löscher, Thomas

AU - Hoelscher, Michael

AU - Berens-Riha, Nicole

PY - 2017

Y1 - 2017

N2 - Due to increased migration, Chagas disease has become an international health problem. Reliable diagnosis of chronically infected people is crucial for prevention of non-vectorial transmission as well as treatment. This study compared four distinct PCR methods for detection of Trypanosoma cruzi DNA for the use in well-equipped routine diagnostic laboratories. DNA was extracted of T. cruzi-positive and negative patients' blood samples and cultured T. cruzi, T. rangeli as well as Leishmania spp. One conventional and two real-time PCR methods targeting a repetitive Sat-DNA sequence as well as one conventional PCR method targeting the variable region of the kDNA minicircle were compared for sensitivity, intra- and interassay precision, limit of detection, specificity and cross-reactivity. Considering the performance, costs and ease of use, an algorithm for PCR-diagnosis of patients with a positive serology for T. cruzi antibodies was developed.

AB - Due to increased migration, Chagas disease has become an international health problem. Reliable diagnosis of chronically infected people is crucial for prevention of non-vectorial transmission as well as treatment. This study compared four distinct PCR methods for detection of Trypanosoma cruzi DNA for the use in well-equipped routine diagnostic laboratories. DNA was extracted of T. cruzi-positive and negative patients' blood samples and cultured T. cruzi, T. rangeli as well as Leishmania spp. One conventional and two real-time PCR methods targeting a repetitive Sat-DNA sequence as well as one conventional PCR method targeting the variable region of the kDNA minicircle were compared for sensitivity, intra- and interassay precision, limit of detection, specificity and cross-reactivity. Considering the performance, costs and ease of use, an algorithm for PCR-diagnosis of patients with a positive serology for T. cruzi antibodies was developed.

KW - Adolescent

KW - Adult

KW - Blood/parasitology

KW - Chagas Disease/diagnosis

KW - Child, Preschool

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Molecular Diagnostic Techniques/methods

KW - Polymerase Chain Reaction/methods

KW - Sensitivity and Specificity

KW - Trypanosoma cruzi/genetics

KW - Young Adult

U2 - 10.1016/j.diagmicrobio.2017.04.003

DO - 10.1016/j.diagmicrobio.2017.04.003

M3 - A1: Web of Science-article

C2 - 28456430

SN - 0732-8893

VL - 88

SP - 225

EP - 232

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 3

ER -

Comparison of four PCR methods for efficient detection of Trypanosoma cruzi in routine diagnostics

Abstract

Keywords

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