Comparison of sensitivities and specificities of latex agglutination and an enzyme-linked immunosorbent assay for detection of antibodies to the human immunodeficiency virus in African sera

HL Francis, M Kabeya, N Kafuama, C Riggins, R Colebunders, R Ryder, J Curran, I Lebughe, TC Quinn

    Research output: Contribution to journalA2: International peer reviewed article (not A1-type)

    Abstract

    The sensitivities, specificities, and positive and negative predictive values of the Cambridge BioScience Corp. (Worcester, Mass.) human immunodeficiency virus latex agglutination assay were compared by using three different blood preparations. By using the manufacturer's standard test method with diluted sera, the sensitivity of latex agglutination was 100%, the specificity was 99.58%, and the positive and negative predictive values were 99.26 and 100%, respectively. Use of diluted whole blood or undiluted whole blood did not significantly affect the sensitivity (mean, 99.72%), specificity (mean, 99.47%), positive predictive value (mean, 99.07%), or negative predictive value (mean, 99.89%). The latex agglutination assay is a simple, rapid assay for the detection of human immunodeficiency virus that would be useful in Third World countries or other areas where enzyme-linked immunosorbent assays are not available or cannot be used
    Original languageEnglish
    JournalJournal of Clinical Microbiology
    Volume26
    Pages (from-to)2462-2464
    ISSN0095-1137
    Publication statusPublished - 1988

    Keywords

    • B780-tropical-medicine
    • Viral diseases
    • AIDS
    • HIV
    • Diagnosis
    • Laboratory medicine
    • Antibodies
    • Western blot
    • Comparative study
    • ELISA
    • Latex fixation tests
    • Congo-Kinshasa
    • Africa-Central

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