Abstract
Two nested PCRs for the detection of Mycobacterium ulcerans were compared by using a collection of 65 clinical specimens. The first method amplifies the gene coding for 16S rRNA, and the second method amplifies a repetitive DNA sequence. The sensitivities of bacterioscopy, culture, 16S rRNA gene PCR, and repetitive-sequence PCR were 29, 34, 80, and 85%, respectively. Compared to the 16S rRNA gene PCR, the repetitive-sequence PCR was faster, easier to perform, and less expensive.
| Original language | English |
|---|---|
| Journal | Journal of Clinical Microbiology |
| Volume | 37 |
| Issue number | 1 |
| Pages (from-to) | 206-208 |
| Number of pages | 3 |
| ISSN | 0095-1137 |
| DOIs | |
| Publication status | Published - 1999 |
Keywords
- B780-tropical-medicine
- Bacteriology
- Mycobacterium ulcerans
- Buruli ulcer
- Laboratory medicine
- Techniques
- Diagnosis
- PCR