Cryopreserved reticulocytes derived from hematopoietic stem cells can be invaded by cryopreserved Plasmodium vivax isolates

F. Noulin, C. Borlon, P. Van den Eede, L. Boel, C.M. Verfaillie, Umberto D'Alessandro, A. Erhart

Research output: Contribution to journalA1: Web of Science-article

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Abstract

The development of a system for the continuous culture of Plasmodium vivax in vitro would benefit from the use of reticulocytes derived from differentiated hematopoietic stem cells (HCS). At present, the need to use both fresh reticulocytes and fresh P. vivax isolates represents a major obstacle towards this goal, particularly for laboratories located in non-endemic countries. Here, we describe a new method for the cryopreservation of HSC-derived reticulocytes to be used for both P. falciparum and P. vivax invasion tests. Cryopreserved P. falciparum and P. vivax isolates could invade both fresh and cryopreserved HSC-derived reticulocytes with similar efficiency. This new technique allows the storage of HSC-derived reticulocytes which can be used for later invasion tests and represents an important step towards the establishment of a continuous P. vivax culture.
Original languageEnglish
JournalPLoS ONE
Volume7
Issue number7
Pages (from-to)e40798
ISSN1932-6203
DOIs
Publication statusPublished - 2012

Keywords

  • Protozoal diseases
  • Malaria
  • Plasmodium falciparum
  • Plasmodium vivax
  • Vectors
  • Mosquitoes
  • Anopheles
  • Cryopreservation
  • Isolation
  • Culture techniques
  • Reticulocytes
  • Hematopoietic stem cells
  • Methods
  • Storage
  • Laboratory techniques and procedures
  • Author

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