TY - JOUR
T1 - Detecting two
Schistosoma circulating antigens - CCA and CAA - in urine and serum to improve diagnosis of human schistosomiasis.
AU - Hoekstra, Pytsje T
AU - de Dood, Claudia J
AU - Abdoel, Theresia
AU - Hilt, Stan
AU - van Diepen, Angela
AU - Polman, Katja
AU - Kremsner, Peter
AU - van Lieshout, Lisette
AU - Kreidenweiss, Andrea
AU - Adegnika, Ayola Akim
AU - Fusco, Daniela
AU - Rasomoelina, Tahinamandranto
AU - Rakoto Andrianarivelo, Mala
AU - Rakotozandrindrainy, Raphaël
AU - Rakotoarivelo, Rivo Andry
AU - Sicuri, Elisa
AU - van Dam, Govert J
AU - Corstjens, Paul L A M
N1 - Copyright © 2024 Hoekstra, de Dood, Abdoel, Hilt, van Diepen, Polman, Kremsner, van Lieshout, Kreidenweiss, Adegnika, Fusco, Rasomoelina, Rakoto Andrianarivelo, Rakotozandrindrainy, Rakotoarivelo, Sicuri, van Dam and Corstjens.
PY - 2024
Y1 - 2024
N2 - BACKGROUND: Schistosomiasis is caused by infection with parasitic
Schistosoma worms and affects more than 250 million people globally. The detection of schistosome derived circulating cathodic and anodic antigens (CCA and CAA) has proven highly valuable for detecting active
Schistosoma infections, causing both intestinal and urinary schistosomiasis.
AIM: The combined detection of CCA and CAA was explored to improve accuracy in detecting
Schistosoma infections.
METHODS: Parallel detection of CCA and CAA was performed on two banked sample sets with matching serum and urine samples from
Schistosoma mansoni (
Sm) and
S. haematobium (
Sh) infected individuals using the non-concentration based lateral flow (LF) test comprising the sensitive luminescent up-converting reporter particle (UCP) technology.
RESULTS: Parallel detection of CCA and CAA increased the positivity rate for detecting both
Sm and
Sh infections compared to the detection of either antigen separately, demonstrating the added value of detecting both antigens in a single sample to confirm diagnosis, independent from the
Schistosoma species. Significantly higher CCA concentrations in urine were observed in
Sm infected individuals compared to
Sh infected individuals, while serum CCA-concentrations were similar between species. CAA concentrations were higher in serum compared to those in urine, irrespective of species. When exploring the relationship of CCA and CAA in urine, the CCA/CAA ratio in
Sm infected individuals was significantly higher than in
Sh infected individuals, while no differences were observed in serum.
DISCUSSION AND CONCLUSION: Parallel detection of CCA and CAA via the UCP-LF platform showed added diagnostic value through an increased positivity rate for the detection of
Sm and
Sh infections, compared to only detecting either of the antigens. The combined and quantitative detection of CCA and CAA is indicative for identifying the infecting species, but needs further exploration.
AB - BACKGROUND: Schistosomiasis is caused by infection with parasitic
Schistosoma worms and affects more than 250 million people globally. The detection of schistosome derived circulating cathodic and anodic antigens (CCA and CAA) has proven highly valuable for detecting active
Schistosoma infections, causing both intestinal and urinary schistosomiasis.
AIM: The combined detection of CCA and CAA was explored to improve accuracy in detecting
Schistosoma infections.
METHODS: Parallel detection of CCA and CAA was performed on two banked sample sets with matching serum and urine samples from
Schistosoma mansoni (
Sm) and
S. haematobium (
Sh) infected individuals using the non-concentration based lateral flow (LF) test comprising the sensitive luminescent up-converting reporter particle (UCP) technology.
RESULTS: Parallel detection of CCA and CAA increased the positivity rate for detecting both
Sm and
Sh infections compared to the detection of either antigen separately, demonstrating the added value of detecting both antigens in a single sample to confirm diagnosis, independent from the
Schistosoma species. Significantly higher CCA concentrations in urine were observed in
Sm infected individuals compared to
Sh infected individuals, while serum CCA-concentrations were similar between species. CAA concentrations were higher in serum compared to those in urine, irrespective of species. When exploring the relationship of CCA and CAA in urine, the CCA/CAA ratio in
Sm infected individuals was significantly higher than in
Sh infected individuals, while no differences were observed in serum.
DISCUSSION AND CONCLUSION: Parallel detection of CCA and CAA via the UCP-LF platform showed added diagnostic value through an increased positivity rate for the detection of
Sm and
Sh infections, compared to only detecting either of the antigens. The combined and quantitative detection of CCA and CAA is indicative for identifying the infecting species, but needs further exploration.
U2 - 10.3389/fpara.2024.1460331
DO - 10.3389/fpara.2024.1460331
M3 - Article
C2 - 39817163
SN - 2813-2424
VL - 3
SP - 1460331
JO - Frontiers in Parasitology
JF - Frontiers in Parasitology
ER -