Detection of HIV-1 antibodies in blood specimens spotted on filter-paper

F Lillo; OE Varnier; E Mantia; A Terragna; G van der Groen; I Van Kerckhoven; PP Mortimer; JV Parry; G Bayliss; H Tamashiro

Detection of HIV-1 antibodies in blood specimens spotted on filter-paper

F Lillo, OE Varnier, E Mantia, A Terragna, G van der Groen, I Van Kerckhoven, PP Mortimer, JV Parry, G Bayliss, H Tamashiro

Institute of Tropical Medicine

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Abstract

Described are the results of an international collaborative study to evaluate the use of whole blood samples spotted on filter-paper (BSP) for the detection of antibodies to human immunodeficiency virus type 1 (HIV-1). BSP samples were collected from 40 patients at risk for HIV-1 infection and tested blindly using commercially available HIV antibody test kits, either specifically manufactured or modified for this purpose. Parallel serum samples were also collected, and the antibody reactivity was defined and confirmed by Western blot. The results demonstrate that recovery of antibodies from BSP samples after elution can be comparable to that from serum. Some kits can be easily adapted to test BSP samples, while others cannot. At present, detection of HIV antibodies in BSP samples should therefore be carried out using kits specifically manufactured for this purpose or by the development of a modified protocol using a panel of BSP and their corresponding serum specimens.

Original language	English
Journal	Bulletin of the World Health Organization
Volume	70
Issue number	3
Pages (from-to)	323-326
Number of pages	4
ISSN	0042-9686
Publication status	Published - 1992

Keywords

B780-tropical-medicine
Virology
HIV-1
Antibody detection
Filter paper

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1992BullWHO323Final published version, 518 KBLicence: Other

https://pmc.ncbi.nlm.nih.gov/articles/PMC2393276/Licence: Other

Cite this

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title = "Detection of HIV-1 antibodies in blood specimens spotted on filter-paper",

abstract = "Described are the results of an international collaborative study to evaluate the use of whole blood samples spotted on filter-paper (BSP) for the detection of antibodies to human immunodeficiency virus type 1 (HIV-1). BSP samples were collected from 40 patients at risk for HIV-1 infection and tested blindly using commercially available HIV antibody test kits, either specifically manufactured or modified for this purpose. Parallel serum samples were also collected, and the antibody reactivity was defined and confirmed by Western blot. The results demonstrate that recovery of antibodies from BSP samples after elution can be comparable to that from serum. Some kits can be easily adapted to test BSP samples, while others cannot. At present, detection of HIV antibodies in BSP samples should therefore be carried out using kits specifically manufactured for this purpose or by the development of a modified protocol using a panel of BSP and their corresponding serum specimens.",

keywords = "B780-tropical-medicine, Virology, HIV-1, Antibody detection, Filter paper",

author = "F Lillo and OE Varnier and E Mantia and A Terragna and {van der Groen}, G and {Van Kerckhoven}, I and PP Mortimer and JV Parry and G Bayliss and H Tamashiro",

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year = "1992",

language = "English",

volume = "70",

pages = "323--326",

journal = "Bulletin of the World Health Organization",

issn = "0042-9686",

publisher = "World Health Organization",

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TY - JOUR

T1 - Detection of HIV-1 antibodies in blood specimens spotted on filter-paper

AU - Lillo, F

AU - Varnier, OE

AU - Mantia, E

AU - Terragna, A

AU - van der Groen, G

AU - Van Kerckhoven, I

AU - Mortimer, PP

AU - Parry, JV

AU - Bayliss, G

AU - Tamashiro, H

N1 - FTX: (CC BY 3.0 IGO) World Health Organization

PY - 1992

Y1 - 1992

N2 - Described are the results of an international collaborative study to evaluate the use of whole blood samples spotted on filter-paper (BSP) for the detection of antibodies to human immunodeficiency virus type 1 (HIV-1). BSP samples were collected from 40 patients at risk for HIV-1 infection and tested blindly using commercially available HIV antibody test kits, either specifically manufactured or modified for this purpose. Parallel serum samples were also collected, and the antibody reactivity was defined and confirmed by Western blot. The results demonstrate that recovery of antibodies from BSP samples after elution can be comparable to that from serum. Some kits can be easily adapted to test BSP samples, while others cannot. At present, detection of HIV antibodies in BSP samples should therefore be carried out using kits specifically manufactured for this purpose or by the development of a modified protocol using a panel of BSP and their corresponding serum specimens.

AB - Described are the results of an international collaborative study to evaluate the use of whole blood samples spotted on filter-paper (BSP) for the detection of antibodies to human immunodeficiency virus type 1 (HIV-1). BSP samples were collected from 40 patients at risk for HIV-1 infection and tested blindly using commercially available HIV antibody test kits, either specifically manufactured or modified for this purpose. Parallel serum samples were also collected, and the antibody reactivity was defined and confirmed by Western blot. The results demonstrate that recovery of antibodies from BSP samples after elution can be comparable to that from serum. Some kits can be easily adapted to test BSP samples, while others cannot. At present, detection of HIV antibodies in BSP samples should therefore be carried out using kits specifically manufactured for this purpose or by the development of a modified protocol using a panel of BSP and their corresponding serum specimens.

KW - B780-tropical-medicine

KW - Virology

KW - HIV-1

KW - Antibody detection

KW - Filter paper

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:A1992JG49100003

M3 - A1: Web of Science-article

SN - 0042-9686

VL - 70

SP - 323

EP - 326

JO - Bulletin of the World Health Organization

JF - Bulletin of the World Health Organization

IS - 3

ER -

Detection of HIV-1 antibodies in blood specimens spotted on filter-paper

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