Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1-seropositive mothers

PN Nyambi; K Fransen; H De Beenhouwer; EN Chomba; M Temmerman; JO Ndinya-Achola; P Piot; G van der Groen

doi:10.1128/JCM.32.11.2858-2860.1994

Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1-seropositive mothers

PN Nyambi, K Fransen, H De Beenhouwer, EN Chomba, M Temmerman, JO Ndinya-Achola, P Piot, G van der Groen

Institute of Tropical Medicine

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

The human immunodeficiency virus type 1 (HIV-1) DNA PCR results of 94 dried blood spot (DBS) samples on filter paper and corresponding venous blood in EDTA obtained from infants born to HIV-1-seropositive mothers were compared. In addition, the results of HIV-1 DNA PCR on DBS and the HIV-1 RNA PCR from plasma of 70 paired samples were compared. A 100% specificity and a 95% sensitivity for HIV-1 DNA PCR on DBS compared with results for venous blood were observed for the 94 paired samples. The results of the DBS HIV-1 DNA PCR and HIV-1 RNA PCR of 70 corresponding plasma samples correlated perfectly (100%). The DBS HIV-1 DNA PCR method proved reliable for HIV-1 detection.

Original language	English
Journal	Journal of Clinical Microbiology
Volume	32
Issue number	11
Pages (from-to)	2858-2860
Number of pages	3
ISSN	0095-1137
DOIs	https://doi.org/10.1128/JCM.32.11.2858-2860.1994
Publication status	Published - 1994

Keywords

B780-tropical-medicine
Virology
HIV-1
Detection
PCR
DNA
RNA
DBS
Laboratory medicine
Diagnosis
Children
Kenya
Africa-East

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10.1128/JCM.32.11.2858-2860.1994

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Nyambi, PN., Fransen, K., De Beenhouwer, H., Chomba, EN., Temmerman, M., Ndinya-Achola, JO., Piot, P., & van der Groen, G. (1994). Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1-seropositive mothers. Journal of Clinical Microbiology, 32(11), 2858-2860. https://doi.org/10.1128/JCM.32.11.2858-2860.1994

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title = "Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1-seropositive mothers",

abstract = "The human immunodeficiency virus type 1 (HIV-1) DNA PCR results of 94 dried blood spot (DBS) samples on filter paper and corresponding venous blood in EDTA obtained from infants born to HIV-1-seropositive mothers were compared. In addition, the results of HIV-1 DNA PCR on DBS and the HIV-1 RNA PCR from plasma of 70 paired samples were compared. A 100% specificity and a 95% sensitivity for HIV-1 DNA PCR on DBS compared with results for venous blood were observed for the 94 paired samples. The results of the DBS HIV-1 DNA PCR and HIV-1 RNA PCR of 70 corresponding plasma samples correlated perfectly (100%). The DBS HIV-1 DNA PCR method proved reliable for HIV-1 detection.",

keywords = "B780-tropical-medicine, Virology, HIV-1, Detection, PCR, DNA, RNA, DBS, Laboratory medicine, Diagnosis, Children, Kenya, Africa-East",

author = "PN Nyambi and K Fransen and {De Beenhouwer}, H and EN Chomba and M Temmerman and JO Ndinya-Achola and P Piot and {van der Groen}, G",

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doi = "10.1128/JCM.32.11.2858-2860.1994",

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Nyambi, PN, Fransen, K, De Beenhouwer, H, Chomba, EN, Temmerman, M, Ndinya-Achola, JO, Piot, P & van der Groen, G 1994, 'Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1-seropositive mothers', Journal of Clinical Microbiology, vol. 32, no. 11, pp. 2858-2860. https://doi.org/10.1128/JCM.32.11.2858-2860.1994

TY - JOUR

T1 - Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1-seropositive mothers

AU - Nyambi, PN

AU - Fransen, K

AU - De Beenhouwer, H

AU - Chomba, EN

AU - Temmerman, M

AU - Ndinya-Achola, JO

AU - Piot, P

AU - van der Groen, G

N1 - FTX: Abonnement

PY - 1994

Y1 - 1994

N2 - The human immunodeficiency virus type 1 (HIV-1) DNA PCR results of 94 dried blood spot (DBS) samples on filter paper and corresponding venous blood in EDTA obtained from infants born to HIV-1-seropositive mothers were compared. In addition, the results of HIV-1 DNA PCR on DBS and the HIV-1 RNA PCR from plasma of 70 paired samples were compared. A 100% specificity and a 95% sensitivity for HIV-1 DNA PCR on DBS compared with results for venous blood were observed for the 94 paired samples. The results of the DBS HIV-1 DNA PCR and HIV-1 RNA PCR of 70 corresponding plasma samples correlated perfectly (100%). The DBS HIV-1 DNA PCR method proved reliable for HIV-1 detection.

AB - The human immunodeficiency virus type 1 (HIV-1) DNA PCR results of 94 dried blood spot (DBS) samples on filter paper and corresponding venous blood in EDTA obtained from infants born to HIV-1-seropositive mothers were compared. In addition, the results of HIV-1 DNA PCR on DBS and the HIV-1 RNA PCR from plasma of 70 paired samples were compared. A 100% specificity and a 95% sensitivity for HIV-1 DNA PCR on DBS compared with results for venous blood were observed for the 94 paired samples. The results of the DBS HIV-1 DNA PCR and HIV-1 RNA PCR of 70 corresponding plasma samples correlated perfectly (100%). The DBS HIV-1 DNA PCR method proved reliable for HIV-1 detection.

KW - B780-tropical-medicine

KW - Virology

KW - HIV-1

KW - Detection

KW - PCR

KW - DNA

KW - RNA

KW - DBS

KW - Laboratory medicine

KW - Diagnosis

KW - Children

KW - Kenya

KW - Africa-East

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:A1994PM49500043

U2 - 10.1128/JCM.32.11.2858-2860.1994

DO - 10.1128/JCM.32.11.2858-2860.1994

M3 - A1: Web of Science-article

SN - 0095-1137

VL - 32

SP - 2858

EP - 2860

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 11

ER -

Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1-seropositive mothers

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Keywords

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