Detection of Leishmania spp. based on the gene encoding HSP20

Ana M Montalvo, Jorge Fraga, Omaira Rodríguez, Orestes Blanco, Alejandro Llanos-Cuentas, Ana L García, Braulio M Valencia, Carlos Muskus, Gert Van der Auwera, José M Requena

Research output: Contribution to journalA2: International peer reviewed article (not A1-type)

Abstract

OBJECTIVES: Explore a new target for molecular diagnosis of Leishmania.

MATERIALS AND METHODS: We evaluated the utility of the gene that encodes the heat shock protein 20-kDa (Hsp20) for detecting Leishmania by polymerase chain reaction (PCR). PCR was normalized and analytical parameters were determined, as well as the validity and diagnostic accuracy, and concordance with the PCR - 18S. PCR-Hsp20 with DNA was obtained from a group of clinical samples from different sources.

RESULTS: The analytical parameters were adequate. The sensitivity obtained was 86% and the specificity was 100%. The concordance with the reference method was good (κ = 0.731), which supports its potential use for diagnosis. The possibility of subsequent identification of the species by sequencing the amplified product gives an additional advantage.

CONCLUSIONS: The usefulness of this gene as a new target for the detection of Leishmania was demonstrated. Because of its potential, it is recommended to improve the sensitivity of the method and to evaluate it in different endemic regions.

Original languageSpanish
JournalRevista Peruana de Medicina Experimental y Salud Pública
Volume31
Issue number4
Pages (from-to)635-643
Number of pages9
ISSN1726-4634
Publication statusPublished - 2014

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