Detection of mpox virus in ambient air in a sexual health clinic

Joren Raymenants, Liesbeth Van Gestel, Jasmine Coppens, Tessa de Block, Eugene Bangwen, Jojanneke Rutgers, Matilde Hens, Elise De Vos, Sandra Coppens, Els Keyaerts, Emmanuel André, Antonio Mauro Rezende, Marjan Van Esbroeck, Koen Vercauteren, Laurens Liesenborghs

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Although transmitted mainly through direct (sexual) contact, mpox virus (MPXV) can be detected in ambient air. We explored the use of air sampling for diagnosis or (genomic) surveillance of mpox in a sexual health clinic. For six out of six patients who were infected with MPXV, all four of our ambient air PCR tests were positive. For 14 uninfected patients, PCR was positive in three ambient air samples, albeit with higher cycle threshold (Ct) values. Genomic sequencing of samples from two positive patients showed matching sequences between air and clinical samples.

Original languageEnglish
Article number210
JournalArchives of Virology
Issue number8
Publication statusPublished - 2023


  • Humans
  • Monkeypox virus
  • Sexual Health
  • Chromosome Mapping
  • Genomics
  • Polymerase Chain Reaction
  • Monkeypox


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