Detection of mpox virus in ambient air in a sexual health clinic

Joren Raymenants; Liesbeth Van Gestel; Jasmine Coppens; Tessa de Block; Eugene Bangwen; Jojanneke Rutgers; Matilde Hens; Elise De Vos; Sandra Coppens; Els Keyaerts; Emmanuel André; Antonio Mauro Rezende; Marjan Van Esbroeck; Koen Vercauteren; Laurens Liesenborghs

doi:10.1007/s00705-023-05837-z

Detection of mpox virus in ambient air in a sexual health clinic

Joren Raymenants, Liesbeth Van Gestel, Jasmine Coppens, Tessa de Block, Eugene Bangwen, Jojanneke Rutgers, Matilde Hens, Elise De Vos, Sandra Coppens, Els Keyaerts, Emmanuel André, Antonio Mauro Rezende, Marjan Van Esbroeck, Koen Vercauteren, Laurens Liesenborghs

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Abstract

Although transmitted mainly through direct (sexual) contact, mpox virus (MPXV) can be detected in ambient air. We explored the use of air sampling for diagnosis or (genomic) surveillance of mpox in a sexual health clinic. For six out of six patients who were infected with MPXV, all four of our ambient air PCR tests were positive. For 14 uninfected patients, PCR was positive in three ambient air samples, albeit with higher cycle threshold (Ct) values. Genomic sequencing of samples from two positive patients showed matching sequences between air and clinical samples.

Original language	English
Article number	210
Journal	Archives of Virology
Volume	168
Issue number	8
ISSN	0304-8608
DOIs	https://doi.org/10.1007/s00705-023-05837-z
Publication status	Published - 2023

Keywords

Humans
Monkeypox virus
Sexual Health
Chromosome Mapping
Genomics
Polymerase Chain Reaction
Monkeypox

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10.1007/s00705-023-05837-zLicence: CC BY

2023avir0210Final published version, 1.2 MBLicence: CC BY

FWO MPX: Op zoek naar het mechanisme achter Monkeypox virus transmissie: een multidisciplinaire 'one health' studie over een opkomende zoönose
Bottieau, E., Liesenborghs, L. & Liesenborghs, L.
Research Fund - Flanders
1/01/22 → 31/12/25
Project: Research Project

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Raymenants, J., Van Gestel, L., Coppens, J., de Block, T., Bangwen, E., Rutgers, J., Hens, M., De Vos, E., Coppens, S., Keyaerts, E., André, E., Rezende, A. M., Van Esbroeck, M., Vercauteren, K., & Liesenborghs, L. (2023). Detection of mpox virus in ambient air in a sexual health clinic. Archives of Virology, 168(8), Article 210. https://doi.org/10.1007/s00705-023-05837-z

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title = "Detection of mpox virus in ambient air in a sexual health clinic",

abstract = "Although transmitted mainly through direct (sexual) contact, mpox virus (MPXV) can be detected in ambient air. We explored the use of air sampling for diagnosis or (genomic) surveillance of mpox in a sexual health clinic. For six out of six patients who were infected with MPXV, all four of our ambient air PCR tests were positive. For 14 uninfected patients, PCR was positive in three ambient air samples, albeit with higher cycle threshold (Ct) values. Genomic sequencing of samples from two positive patients showed matching sequences between air and clinical samples.",

keywords = "Humans, Monkeypox virus, Sexual Health, Chromosome Mapping, Genomics, Polymerase Chain Reaction, Monkeypox",

author = "Joren Raymenants and {Van Gestel}, Liesbeth and Jasmine Coppens and {de Block}, Tessa and Eugene Bangwen and Jojanneke Rutgers and Matilde Hens and {De Vos}, Elise and Sandra Coppens and Els Keyaerts and Emmanuel Andr{\'e} and Rezende, {Antonio Mauro} and {Van Esbroeck}, Marjan and Koen Vercauteren and Laurens Liesenborghs",

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year = "2023",

doi = "10.1007/s00705-023-05837-z",

language = "English",

volume = "168",

journal = "Archives of Virology",

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T1 - Detection of mpox virus in ambient air in a sexual health clinic

AU - Raymenants, Joren

AU - Van Gestel, Liesbeth

AU - Coppens, Jasmine

AU - de Block, Tessa

AU - Bangwen, Eugene

AU - Rutgers, Jojanneke

AU - Hens, Matilde

AU - De Vos, Elise

AU - Coppens, Sandra

AU - Keyaerts, Els

AU - André, Emmanuel

AU - Rezende, Antonio Mauro

AU - Van Esbroeck, Marjan

AU - Vercauteren, Koen

AU - Liesenborghs, Laurens

PY - 2023

Y1 - 2023

N2 - Although transmitted mainly through direct (sexual) contact, mpox virus (MPXV) can be detected in ambient air. We explored the use of air sampling for diagnosis or (genomic) surveillance of mpox in a sexual health clinic. For six out of six patients who were infected with MPXV, all four of our ambient air PCR tests were positive. For 14 uninfected patients, PCR was positive in three ambient air samples, albeit with higher cycle threshold (Ct) values. Genomic sequencing of samples from two positive patients showed matching sequences between air and clinical samples.

AB - Although transmitted mainly through direct (sexual) contact, mpox virus (MPXV) can be detected in ambient air. We explored the use of air sampling for diagnosis or (genomic) surveillance of mpox in a sexual health clinic. For six out of six patients who were infected with MPXV, all four of our ambient air PCR tests were positive. For 14 uninfected patients, PCR was positive in three ambient air samples, albeit with higher cycle threshold (Ct) values. Genomic sequencing of samples from two positive patients showed matching sequences between air and clinical samples.

KW - Humans

KW - Monkeypox virus

KW - Sexual Health

KW - Chromosome Mapping

KW - Genomics

KW - Polymerase Chain Reaction

KW - Monkeypox

U2 - 10.1007/s00705-023-05837-z

DO - 10.1007/s00705-023-05837-z

M3 - A1: Web of Science-article

C2 - 37486383

SN - 0304-8608

VL - 168

JO - Archives of Virology

JF - Archives of Virology

IS - 8

M1 - 210

ER -

Detection of mpox virus in ambient air in a sexual health clinic

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Keywords

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FWO MPX: Op zoek naar het mechanisme achter Monkeypox virus transmissie: een multidisciplinaire 'one health' studie over een opkomende zoönose

Cite this