Detection of salivary HIV-1-specific IgG antibodies in high-risk populations in Zaire

FM Behets, E Bazepeyo, TC Quinn, A Limbaya, B Kagoyire, N Nzilambi, M Laga, P Piot, RW Ryder, CC Brown

Research output: Contribution to journalA1: Web of Science-article

Abstract

Saliva and blood samples were tested for human immunodeficiency virus-1 (HIV-1) antibodies in two high-risk populations in Kinshasa, Zaire. In a seroprevalence study of 458 sexually transmitted disease (STD) clinic attendees, 142 of 145 seropositive individuals had enzyme-linked immunosorbent assay (ELISA)-positive saliva samples (97.9% sensitivity). All saliva samples from seronegative patients were ELISA-negative (100% specificity). Of the 142 ELISA-positive saliva specimens, 137 were also Western blot-positive (94.5% sensitivity). In a subsequent seroincidence study of 315 initially seronegative female prostitutes followed during 183 woman-years of observation, 9 of 14 women who seroconverted (7.7% seroincidence) had ELISA-positive saliva samples at the time seroconversion was detected. Only three of these saliva specimens could be confirmed by Western blot. Although salivary testing for HIV-1 antibodies using conventional assays was not sensitive in detecting recent seroconversions, screening of salivary samples for HIV-1 antibody provides a convenient alternative method for conducting seroprevalence surveys in populations in whom venipuncture is not possible or convenient
Original languageEnglish
JournalJournal of Acquired Immune Deficiency Syndromes
Volume4
Pages (from-to)183-187
ISSN1525-4135
Publication statusPublished - 1991

Keywords

  • B780-tropical-medicine
  • Congo-Kinshasa
  • Virology
  • Laboratory
  • HIV
  • Saliva
  • Immunoglobulin G
  • Antibodies
  • ELISA
  • Western blot
  • Seroprevalence
  • Seroincidence
  • Kinshasa
  • Africa-Central

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