Background. Persistence of Zika virus (ZIKV) ribonucleic acid (RNA) in semen is common after infection.
Methods. We designed a reverse-transcription polymerase chain reaction assay that targets antisense ZIKV RNA (asRNA) to assess ZIKV replication competence in ZIKV RNA-positive semen samples.
Results. We detected ZIKV asRNA in semen of 9 of 19 men (47.4%) diagnosed with ZIKV infection. All asRNA-positive samples had high ZIKV loads (cycle threshold values
Conclusions. The sensitivity of the asRNA assay for detection of ZIKV replication was higher than that of conventional virus isolation methods (47.4% vs 21.1%, P = .032).
- antisense RNA
- reverse-transcription polymerase chain reaction
- sexual transmission
- Zika virus
- NEGATIVE STRAND