Detection of Zika virus replication in human semen by reverse transcription polymerase chain reaction targeting of antisense ribonucleic acid

Ralph Huits; Birgit De Smet; Gilda Grard; Kaat Eggermont; Catherine Minto-Bain; Natalie Jess; Isabelle Leparc-Goffart; Denis Malvy; Lieselotte Cnops

doi:10.1093/infdis/jiaa070

Detection of Zika virus replication in human semen by reverse transcription polymerase chain reaction targeting of antisense ribonucleic acid

Ralph Huits, Birgit De Smet, Gilda Grard, Kaat Eggermont, Catherine Minto-Bain, Natalie Jess, Isabelle Leparc-Goffart, Denis Malvy, Lieselotte Cnops

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Abstract

Background. Persistence of Zika virus (ZIKV) ribonucleic acid (RNA) in semen is common after infection.

Methods. We designed a reverse-transcription polymerase chain reaction assay that targets antisense ZIKV RNA (asRNA) to assess ZIKV replication competence in ZIKV RNA-positive semen samples.

Results. We detected ZIKV asRNA in semen of 9 of 19 men (47.4%) diagnosed with ZIKV infection. All asRNA-positive samples had high ZIKV loads (cycle threshold values

Conclusions. The sensitivity of the asRNA assay for detection of ZIKV replication was higher than that of conventional virus isolation methods (47.4% vs 21.1%, P = .032).

Original language	English
Journal	Journal of Infectious Diseases
Volume	222
Issue number	2
Pages (from-to)	319-323
Number of pages	5
ISSN	0022-1899
DOIs	https://doi.org/10.1093/infdis/jiaa070
Publication status	Published - 2020

Keywords

antisense RNA
reverse-transcription polymerase chain reaction
semen
sexual transmission
Zika virus
NEGATIVE STRAND
PERSISTENCE

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title = "Detection of Zika virus replication in human semen by reverse transcription polymerase chain reaction targeting of antisense ribonucleic acid",

abstract = "Background. Persistence of Zika virus (ZIKV) ribonucleic acid (RNA) in semen is common after infection.Methods. We designed a reverse-transcription polymerase chain reaction assay that targets antisense ZIKV RNA (asRNA) to assess ZIKV replication competence in ZIKV RNA-positive semen samples.Results. We detected ZIKV asRNA in semen of 9 of 19 men (47.4%) diagnosed with ZIKV infection. All asRNA-positive samples had high ZIKV loads (cycle threshold valuesConclusions. The sensitivity of the asRNA assay for detection of ZIKV replication was higher than that of conventional virus isolation methods (47.4% vs 21.1%, P = .032).",

keywords = "antisense RNA, reverse-transcription polymerase chain reaction, semen, sexual transmission, Zika virus, NEGATIVE STRAND, PERSISTENCE",

author = "Ralph Huits and {De Smet}, Birgit and Gilda Grard and Kaat Eggermont and Catherine Minto-Bain and Natalie Jess and Isabelle Leparc-Goffart and Denis Malvy and Lieselotte Cnops",

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year = "2020",

doi = "10.1093/infdis/jiaa070",

language = "English",

volume = "222",

pages = "319--323",

journal = "Journal of Infectious Diseases",

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publisher = "Oxford University Press",

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TY - JOUR

T1 - Detection of Zika virus replication in human semen by reverse transcription polymerase chain reaction targeting of antisense ribonucleic acid

AU - Huits, Ralph

AU - De Smet, Birgit

AU - Grard, Gilda

AU - Eggermont, Kaat

AU - Minto-Bain, Catherine

AU - Jess, Natalie

AU - Leparc-Goffart, Isabelle

AU - Malvy, Denis

AU - Cnops, Lieselotte

N1 - FTX

PY - 2020

Y1 - 2020

N2 - Background. Persistence of Zika virus (ZIKV) ribonucleic acid (RNA) in semen is common after infection.Methods. We designed a reverse-transcription polymerase chain reaction assay that targets antisense ZIKV RNA (asRNA) to assess ZIKV replication competence in ZIKV RNA-positive semen samples.Results. We detected ZIKV asRNA in semen of 9 of 19 men (47.4%) diagnosed with ZIKV infection. All asRNA-positive samples had high ZIKV loads (cycle threshold valuesConclusions. The sensitivity of the asRNA assay for detection of ZIKV replication was higher than that of conventional virus isolation methods (47.4% vs 21.1%, P = .032).

AB - Background. Persistence of Zika virus (ZIKV) ribonucleic acid (RNA) in semen is common after infection.Methods. We designed a reverse-transcription polymerase chain reaction assay that targets antisense ZIKV RNA (asRNA) to assess ZIKV replication competence in ZIKV RNA-positive semen samples.Results. We detected ZIKV asRNA in semen of 9 of 19 men (47.4%) diagnosed with ZIKV infection. All asRNA-positive samples had high ZIKV loads (cycle threshold valuesConclusions. The sensitivity of the asRNA assay for detection of ZIKV replication was higher than that of conventional virus isolation methods (47.4% vs 21.1%, P = .032).

KW - antisense RNA

KW - reverse-transcription polymerase chain reaction

KW - semen

KW - sexual transmission

KW - Zika virus

KW - NEGATIVE STRAND

KW - PERSISTENCE

U2 - 10.1093/infdis/jiaa070

DO - 10.1093/infdis/jiaa070

M3 - A1: Web of Science-article

SN - 0022-1899

VL - 222

SP - 319

EP - 323

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 2

ER -

Detection of Zika virus replication in human semen by reverse transcription polymerase chain reaction targeting of antisense ribonucleic acid

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Keywords

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