Detection of Leptomonas sp. parasites in clinical isolates of Kala-azar patients from India

P Srivastava; VK Prajapati; M Vanaerschot; G Van der Auwera; JC Dujardin; S Sundar

doi:10.1016/j.meegid.2010.07.009

Detection of Leptomonas sp. parasites in clinical isolates of Kala-azar patients from India

P Srivastava, VK Prajapati, M Vanaerschot, G Van der Auwera, JC Dujardin, S Sundar

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

We report here nine unusual cases of Kala-azar, of which parasites were isolated and found by 18S rRNA gene sequencing to be most similar to Leptomonas species. One of these isolates was used to inoculate Balb/c mice; organs were collected and directly submitted to a genus-specific rDNA-ITS1 PCR analysis: this revealed the presence of both Leptomonas sp. and Leishmania donovani. Therefore, we conclude that there was a mixed infection of Leptomonas sp. and L. donovani in this isolate. We consider that mixed infection may be present in the patients themselves, Leptomonas persisting in them because of the immuno-suppression associated with Kala-azar

Original language	English
Journal	Infection, Genetics and Evolution
Volume	10
Issue number	7
Pages (from-to)	1145-1150
Number of pages	6
ISSN	1567-1348
DOIs	https://doi.org/10.1016/j.meegid.2010.07.009
Publication status	Published - 2010

Keywords

B780-tropical-medicine
Protozoal diseases
Leishmaniasis
Visceral
Kala azar
Leishmania donovani
Leptomonas
Mixed infections
Detection
Gene sequencing
RNA
Immunosuppression
Humans
Mice
Isolation
India
Asia-South

Access to Document

10.1016/j.meegid.2010.07.009

http://lib.itg.be/pdf/itg/2010/2010igev1145.pdf

Cite this

@article{bf314ac2ab3147649d9dd583dd3c0978,

title = "Detection of Leptomonas sp. parasites in clinical isolates of Kala-azar patients from India",

abstract = "We report here nine unusual cases of Kala-azar, of which parasites were isolated and found by 18S rRNA gene sequencing to be most similar to Leptomonas species. One of these isolates was used to inoculate Balb/c mice; organs were collected and directly submitted to a genus-specific rDNA-ITS1 PCR analysis: this revealed the presence of both Leptomonas sp. and Leishmania donovani. Therefore, we conclude that there was a mixed infection of Leptomonas sp. and L. donovani in this isolate. We consider that mixed infection may be present in the patients themselves, Leptomonas persisting in them because of the immuno-suppression associated with Kala-azar",

keywords = "B780-tropical-medicine, Protozoal diseases, Leishmaniasis, Visceral, Kala azar, Leishmania donovani, Leptomonas, Mixed infections, Detection, Gene sequencing, RNA, Immunosuppression, Humans, Mice, Isolation, India, Asia-South",

author = "P Srivastava and VK Prajapati and M Vanaerschot and {Van der Auwera}, G and JC Dujardin and S Sundar",

note = "ITG-P3B; ITG-P4B; ITG-P5A; PARAS; U-PROTO; JIF; DOI; Abstract; PDF; DSPACE",

year = "2010",

doi = "10.1016/j.meegid.2010.07.009",

language = "English",

volume = "10",

pages = "1145--1150",

journal = "Infection, Genetics and Evolution",

issn = "1567-1348",

publisher = "Elsevier",

number = "7",

}

TY - JOUR

T1 - Detection of Leptomonas sp. parasites in clinical isolates of Kala-azar patients from India

AU - Srivastava, P

AU - Prajapati, VK

AU - Vanaerschot, M

AU - Van der Auwera, G

AU - Dujardin, JC

AU - Sundar, S

N1 - ITG-P3B; ITG-P4B; ITG-P5A; PARAS; U-PROTO; JIF; DOI; Abstract; PDF; DSPACE

PY - 2010

Y1 - 2010

N2 - We report here nine unusual cases of Kala-azar, of which parasites were isolated and found by 18S rRNA gene sequencing to be most similar to Leptomonas species. One of these isolates was used to inoculate Balb/c mice; organs were collected and directly submitted to a genus-specific rDNA-ITS1 PCR analysis: this revealed the presence of both Leptomonas sp. and Leishmania donovani. Therefore, we conclude that there was a mixed infection of Leptomonas sp. and L. donovani in this isolate. We consider that mixed infection may be present in the patients themselves, Leptomonas persisting in them because of the immuno-suppression associated with Kala-azar

AB - We report here nine unusual cases of Kala-azar, of which parasites were isolated and found by 18S rRNA gene sequencing to be most similar to Leptomonas species. One of these isolates was used to inoculate Balb/c mice; organs were collected and directly submitted to a genus-specific rDNA-ITS1 PCR analysis: this revealed the presence of both Leptomonas sp. and Leishmania donovani. Therefore, we conclude that there was a mixed infection of Leptomonas sp. and L. donovani in this isolate. We consider that mixed infection may be present in the patients themselves, Leptomonas persisting in them because of the immuno-suppression associated with Kala-azar

KW - B780-tropical-medicine

KW - Protozoal diseases

KW - Leishmaniasis

KW - Visceral

KW - Kala azar

KW - Leishmania donovani

KW - Leptomonas

KW - Mixed infections

KW - Detection

KW - Gene sequencing

KW - RNA

KW - Immunosuppression

KW - Humans

KW - Mice

KW - Isolation

KW - India

KW - Asia-South

U2 - 10.1016/j.meegid.2010.07.009

DO - 10.1016/j.meegid.2010.07.009

M3 - A1: Web of Science-article

C2 - 20633704

SN - 1567-1348

VL - 10

SP - 1145

EP - 1150

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

IS - 7

ER -

Detection of Leptomonas sp. parasites in clinical isolates of Kala-azar patients from India

Abstract

Keywords

Access to Document

Fingerprint

Cite this