Detection of Leptomonas sp. parasites in clinical isolates of Kala-azar patients from India

P Srivastava, VK Prajapati, M Vanaerschot, G Van der Auwera, JC Dujardin, S Sundar

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    We report here nine unusual cases of Kala-azar, of which parasites were isolated and found by 18S rRNA gene sequencing to be most similar to Leptomonas species. One of these isolates was used to inoculate Balb/c mice; organs were collected and directly submitted to a genus-specific rDNA-ITS1 PCR analysis: this revealed the presence of both Leptomonas sp. and Leishmania donovani. Therefore, we conclude that there was a mixed infection of Leptomonas sp. and L. donovani in this isolate. We consider that mixed infection may be present in the patients themselves, Leptomonas persisting in them because of the immuno-suppression associated with Kala-azar
    Original languageEnglish
    JournalInfection, Genetics and Evolution
    Volume10
    Issue number7
    Pages (from-to)1145-1150
    Number of pages6
    ISSN1567-1348
    DOIs
    Publication statusPublished - 2010

    Keywords

    • B780-tropical-medicine
    • Protozoal diseases
    • Leishmaniasis
    • Visceral
    • Kala azar
    • Leishmania donovani
    • Leptomonas
    • Mixed infections
    • Detection
    • Gene sequencing
    • RNA
    • Immunosuppression
    • Humans
    • Mice
    • Isolation
    • India
    • Asia-South

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