Development of a slide ELISA for canine leishmaniasis and comparison with four serological tests

F Vercammen; D Berkvens; D Le Ray; D Jacquet; T Vervoort

doi:10.1136/vr.141.13.328

Development of a slide ELISA for canine leishmaniasis and comparison with four serological tests

F Vercammen, D Berkvens, D Le Ray, D Jacquet, T Vervoort

Institute of Tropical Medicine

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

A slide ELISA for canine leishmaniasis was developed by using promastigotes of Leishmania infantum, and compared with microimmunodiffusion, immunoelectrophoresis, direct agglutination and indirect immunofluorescence assays. The sensitivity of all the tests was 100 per cent. The specificity of the direct agglutination test was 95 per cent but it was 100 per cent for the three other tests. There was also a positive correlation and a high level of concordance between the titres measured by the different tests.

Original language	English
Journal	Veterinary Record
Volume	141
Issue number	13
Pages (from-to)	328-330
Number of pages	3
ISSN	0042-4900
DOIs	https://doi.org/10.1136/vr.141.13.328
Publication status	Published - 1997

Keywords

B780-tropical-medicine
Protozoal diseases
Leishmaniasis
Serology
Laboratory medicine
Techniques
ELISA
Canine
Dogs
Animal diseases
SELISA

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10.1136/vr.141.13.328

http://lib.itg.be/pdf/itg/1997/1997vrec0328.pdf

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title = "Development of a slide ELISA for canine leishmaniasis and comparison with four serological tests",

abstract = "A slide ELISA for canine leishmaniasis was developed by using promastigotes of Leishmania infantum, and compared with microimmunodiffusion, immunoelectrophoresis, direct agglutination and indirect immunofluorescence assays. The sensitivity of all the tests was 100 per cent. The specificity of the direct agglutination test was 95 per cent but it was 100 per cent for the three other tests. There was also a positive correlation and a high level of concordance between the titres measured by the different tests.",

keywords = "B780-tropical-medicine, Protozoal diseases, Leishmaniasis, Serology, Laboratory medicine, Techniques, ELISA, Canine, Dogs, Animal diseases, SELISA",

author = "F Vercammen and D Berkvens and {Le Ray}, D and D Jacquet and T Vervoort",

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year = "1997",

doi = "10.1136/vr.141.13.328",

language = "English",

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TY - JOUR

T1 - Development of a slide ELISA for canine leishmaniasis and comparison with four serological tests

AU - Vercammen, F

AU - Berkvens, D

AU - Le Ray, D

AU - Jacquet, D

AU - Vervoort, T

N1 - FTX: Abonnement

PY - 1997

Y1 - 1997

N2 - A slide ELISA for canine leishmaniasis was developed by using promastigotes of Leishmania infantum, and compared with microimmunodiffusion, immunoelectrophoresis, direct agglutination and indirect immunofluorescence assays. The sensitivity of all the tests was 100 per cent. The specificity of the direct agglutination test was 95 per cent but it was 100 per cent for the three other tests. There was also a positive correlation and a high level of concordance between the titres measured by the different tests.

AB - A slide ELISA for canine leishmaniasis was developed by using promastigotes of Leishmania infantum, and compared with microimmunodiffusion, immunoelectrophoresis, direct agglutination and indirect immunofluorescence assays. The sensitivity of all the tests was 100 per cent. The specificity of the direct agglutination test was 95 per cent but it was 100 per cent for the three other tests. There was also a positive correlation and a high level of concordance between the titres measured by the different tests.

KW - B780-tropical-medicine

KW - Protozoal diseases

KW - Leishmaniasis

KW - Serology

KW - Laboratory medicine

KW - Techniques

KW - ELISA

KW - Canine

KW - Dogs

KW - Animal diseases

KW - SELISA

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:A1997YB48400007

U2 - 10.1136/vr.141.13.328

DO - 10.1136/vr.141.13.328

M3 - A1: Web of Science-article

SN - 0042-4900

VL - 141

SP - 328

EP - 330

JO - Veterinary Record

JF - Veterinary Record

IS - 13

ER -

Development of a slide ELISA for canine leishmaniasis and comparison with four serological tests

Abstract

Keywords

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