Development of an enzyme-linked immunosorbent assay to identify host-feeding preferences of Phlebotomus species (Diptera: Psychodidae) in endemic foci of visceral leishmaniasis in Nepal

I Burniston, L Roy, A Picado, M Das, S Rijal, M Rogers, M Coosemans, M Boelaert, C Davies, M Cameron

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    Anthroponotic visceral leishmaniasis, transmitted by Phlebotomus argentipes Annandale & Brunetti (Diptera: Psychodidae) sand flies, is regarded as a major problem of public healthimportance in the Indian subcontinent. Understanding the feeding behavior of the vector can be used to investigate changes in human-vector contact during intervention programs. An enzyme-linked immunosorbent assay (ELISA) was modified to make it suitable to identify the origin of P. argentipes and Phlebotomus papatasi Scopoli (Diptera: Psychodidae) blood meals. The sensitivity and specificity of the precipitin ring test and ELISA were compared, as well as the stability of the tests across different stages of blood meal digestion. The ELISA was more sensitive and specific than the precipitin test for identifying the sources of blood meals. When using the ELISA method with a plate reader, it was possible to obtain 100% sensitivity and specificity. When comparing the techniques across digestion stages, it was found that there was a drop in sensitivity, 48 and 72 h postblood meal for precipitin and visually read ELISA, respectively. However, the sensitivity of the ELISA using a plate reader was not altered by the digestion time. The feeding habits of P. argentipes and P. papatasi from the Terai region of Nepal, determined by the ELISA developed, showed P. papatasi to be highly anthropophilic, and P. argentipes appeared to feed both on humans and animals, in particular bovines.
    Original languageEnglish
    JournalJournal of Medical Entomology
    Volume47
    Issue number5
    Pages (from-to)902-906
    Number of pages5
    ISSN0022-2585
    DOIs
    Publication statusPublished - 2010

    Keywords

    • B780-tropical-medicine
    • Protozoal diseases
    • Leishmaniasis
    • Visceral
    • Kala azar
    • Leishmania donovani
    • Vectors
    • Sandflies
    • Phlebotomus argentipes
    • Phlebotomus papatasi
    • Vector ecology
    • Identification
    • Enzyme-linked immunosorbent assay (ELISA)
    • ELISA
    • Sensitivity
    • Specificity
    • Feeding habits
    • Feeding behavior
    • Nepal
    • Asia-South

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