Diagnostic accuracy and feasibility of serological tests on filter paper samples for outbreak detection of T.b. gambiense Human African Trypanosomiasis

E Hasker, P Lutumba, D Mumba, V Lejon, P Büscher, V Kande, JJ Muyembe, J Menten, J Robays, M Boelaert

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    Control of human African trypanosomiasis (HAT) in the Democratic Republic of Congo is based on mass population screening by mobile teams; a costly and labor-intensive approach. We hypothesized that blood samples collected on filter paper by village health workers and processed in a central laboratory might be a cost-effective alternative. We estimated sensitivity and specificity of micro-card agglutination test for trypanosomiasis (micro-CATT) and enzyme-linked immunosorbent assay (ELISA)/T.b. gambiense on filter paper samples compared with parasitology-based case classification and used the results in a Monte Carlo simulation of a lot quality assurance sampling (LQAS) approach. Micro-CATT and ELISA/T.b. gambiense showed acceptable sensitivity (92.7% [95% CI 87.4-98.0%] and 82.2% [95% CI 75.3-90.4%]) and very high specificity (99.4% [95% CI 99.0-99.9%] and 99.8% [95% CI 99.5-100%]), respectively. Conditional on high sample size per lot (>/= 60%), both tests could reliably distinguish a 2% from a zero prevalence at village level. Alternatively, these tests could be used to identify individual HAT suspects for subsequent confirmation
    Original languageEnglish
    JournalAmerican Journal of Tropical Medicine and Hygiene
    Volume83
    Issue number2
    Pages (from-to)374-379
    Number of pages6
    ISSN0002-9637
    DOIs
    Publication statusPublished - 2010

    Keywords

    • B780-tropical-medicine
    • Protozoal diseases
    • Trypanosomiasis-African
    • Sleeping sickness
    • Trypanosoma brucei gambiense
    • Vectors
    • Tsetse flies
    • Evaluation
    • Rapid diagnostic tests
    • Diagnostics
    • Serological
    • Test kits
    • Filter paper
    • Accuracy
    • Feasibility
    • Detection
    • Outbreaks
    • Sensitivity
    • Specificity
    • CATT
    • ELISA
    • Congo-Kinshasa
    • Africa-Central

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