Diagnostic accuracy of PCR in gambiense sleeping sickness diagnosis, staging and post-treatment follow-up: a 2-year longitudinal study

S Deborggraeve, V Lejon, RA Ekangu, D Mumba Ngoyi, PP Pyana, M Ilunga, JP Mulunda, P Büscher

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    BACKGROUND: The polymerase chain reaction (PCR) has been proposed for diagnosis, staging and post-treatment follow-up of sleeping sickness but no large-scale clinical evaluations of its diagnostic accuracy have taken place yet. METHODOLOGY/PRINCIPAL FINDINGS: An 18S ribosomal RNA gene targeting PCR was performed on blood and cerebrospinal fluid (CSF) of 360 T. brucei gambiense sleeping sickness patients and on blood of 129 endemic controls from the Democratic Republic of Congo. Sensitivity and specificity (with 95% confidence intervals) of PCR for diagnosis, disease staging and treatment failure over 2 years follow-up post-treatment were determined. Reference standard tests were trypanosome detection for diagnosis and trypanosome detection and/or increased white blood cell concentration in CSF for staging and detection of treatment failure. PCR on blood showed a sensitivity of 88.4% (84.4-92.5%) and a specificity of 99.2% (97.7-100%) for diagnosis, while for disease staging the sensitivity and specificity of PCR on cerebrospinal fluid were 88.4% (84.8-91.9%) and 82.9% (71.2-94.6%), respectively. During follow-up after treatment, PCR on blood had low sensitivity to detect treatment failure. In cerebrospinal fluid, PCR positivity vanished slowly and was observed until the end of the 2 year follow-up in around 20% of successfully treated patients. CONCLUSIONS/SIGNIFICANCE: For T.b. gambiense sleeping sickness diagnosis and staging, PCR performed better than, or similar to, the current parasite detection techniques but it cannot be used for post-treatment follow-up. Continued PCR positivity in one out of five cured patients points to persistence of living or dead parasites or their DNA after successful treatment and may necessitate the revision of some paradigms about the pathophysiology of sleeping sickness.
    Original languageEnglish
    JournalPLoS Neglected Tropical Diseases
    Volume5
    Issue number2
    Pages (from-to)e972
    Number of pages7
    ISSN1935-2727
    DOIs
    Publication statusPublished - 2011

    Keywords

    • B780-tropical-medicine
    • Protozoal diseases
    • Trypanosomiasis-African
    • Sleeping sickness
    • Trypanosoma brucei gambiense
    • Vectors
    • Tsetse flies
    • Glossina
    • Diagnosis
    • Cerebrospinal fluid
    • Stage determination
    • Staging
    • Follow-up
    • Polymerase chain reaction
    • PCR
    • Accuracy
    • Sensitivity
    • Specificity
    • Laboratory techniques and procedures
    • Congo-Kinshasa
    • Africa-Central

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