Diagnostic evaluation of PCR in goats experimentally infected with Trypanosoma vivax

PJL Pereira de Almeida; M Ndao; N Van Meirvenne; S Geerts

doi:10.1016/S0001-706X(97)00677-3

Diagnostic evaluation of PCR in goats experimentally infected with Trypanosoma vivax

PJL Pereira de Almeida
, M Ndao
, N Van Meirvenne
, S Geerts

Institute of Tropical Medicine

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Six goats were experimentally infected with a stock of Trypanosoma vivax. Parasitaemia was weekly monitored by buffy coat and wet blood film examination during a period of 15 weeks and another 3 weeks following drug-treatment. Dried blood samples were tested by the polymerase chain reaction (PCR), using an extraction method with Chelex® 100 (BioRad). PCR proved consistently more sensitive than the parasitological techniques.

Original language	English
Journal	Acta Tropica
Volume	66
Issue number	1
Pages (from-to)	45-50
Number of pages	6
ISSN	0001-706X
DOIs	https://doi.org/10.1016/S0001-706X(97)00677-3
Publication status	Published - 1997

Keywords

B780-tropical-medicine
Protozoal diseases
Animal diseases
Trypanosoma vivax
Experimental
PCR
Goats
Laboratory medicine
Diagnosis
Chelex

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10.1016/S0001-706X(97)00677-3

http://lib.itg.be/pdf/itg/1997/1997atro0045.pdf

Cite this

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title = "Diagnostic evaluation of PCR in goats experimentally infected with Trypanosoma vivax",

abstract = "Six goats were experimentally infected with a stock of Trypanosoma vivax. Parasitaemia was weekly monitored by buffy coat and wet blood film examination during a period of 15 weeks and another 3 weeks following drug-treatment. Dried blood samples were tested by the polymerase chain reaction (PCR), using an extraction method with Chelex{\textregistered} 100 (BioRad). PCR proved consistently more sensitive than the parasitological techniques.",

keywords = "B780-tropical-medicine, Protozoal diseases, Animal diseases, Trypanosoma vivax, Experimental, PCR, Goats, Laboratory medicine, Diagnosis, Chelex",

author = "\{Pereira de Almeida\}, PJL and M Ndao and \{Van Meirvenne\}, N and S Geerts",

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year = "1997",

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T1 - Diagnostic evaluation of PCR in goats experimentally infected with Trypanosoma vivax

AU - Pereira de Almeida, PJL

AU - Ndao, M

AU - Van Meirvenne, N

AU - Geerts, S

N1 - FTX: Abonnement

PY - 1997

Y1 - 1997

N2 - Six goats were experimentally infected with a stock of Trypanosoma vivax. Parasitaemia was weekly monitored by buffy coat and wet blood film examination during a period of 15 weeks and another 3 weeks following drug-treatment. Dried blood samples were tested by the polymerase chain reaction (PCR), using an extraction method with Chelex® 100 (BioRad). PCR proved consistently more sensitive than the parasitological techniques.

AB - Six goats were experimentally infected with a stock of Trypanosoma vivax. Parasitaemia was weekly monitored by buffy coat and wet blood film examination during a period of 15 weeks and another 3 weeks following drug-treatment. Dried blood samples were tested by the polymerase chain reaction (PCR), using an extraction method with Chelex® 100 (BioRad). PCR proved consistently more sensitive than the parasitological techniques.

KW - B780-tropical-medicine

KW - Protozoal diseases

KW - Animal diseases

KW - Trypanosoma vivax

KW - Experimental

KW - PCR

KW - Goats

KW - Laboratory medicine

KW - Diagnosis

KW - Chelex

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:A1997XB86500005

U2 - 10.1016/S0001-706X(97)00677-3

DO - 10.1016/S0001-706X(97)00677-3

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SN - 0001-706X

VL - 66

SP - 45

EP - 50

JO - Acta Tropica

JF - Acta Tropica

IS - 1

ER -

Diagnostic evaluation of PCR in goats experimentally infected with Trypanosoma vivax

Abstract

Keywords

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