Differences in T-cell responses between Mycobacterium tuberculosis and Mycobacterium africanum-infected patients

Leopold D Tientcheu, Jayne S Sutherland, Bouke de Jong, Beate Kampmann, James Jafali, Ifedayo M Adetifa, Martin Antonio, Hazel M Dockrell, Martin O Ota

Research output: Contribution to journalA1: Web of Science-article

Abstract

In The Gambia, Mycobacterium tuberculosis (Mtb) and Mycobacterium africanum (Maf) are major causes of tuberculosis (TB). Maf is more likely to cause TB in immune suppressed individuals, implying differences in virulence. Despite this, few studies have assessed the underlying immunity to the two pathogens in human. In this study, we analyzed T-cell responses from 19 Maf- and 29 Mtb-infected HIV-negative patients before and after TB chemotherapy following overnight stimulation of whole blood with TB-specific antigens. Before treatment, percentages of early secreted antigenic target-6(ESAT-6)/culture filtrate protein-10(CFP-10) and purified protein derivative-specific single-TNF-α-producing CD4(+) and CD8(+) T cells were significantly higher while single-IL-2-producing T cells were significantly lower in Maf- compared with Mtb-infected patients. Purified protein derivative-specific polyfunctional CD4(+) T cells frequencies were significantly higher before than after treatment, but there was no difference between the groups at both time points. Furthermore, the proportion of CD3(+) CD11b(+) T cells was similar in both groups pretreatment, but was significantly lower with higher TNF-α, IL-2, and IFN-γ production in Mtb- compared with that of Maf-infected patients posttreatment. Our data provide evidence of differences in T-cell responses to two mycobacterial strains with differing virulence, providing some insight into TB pathogenesis with different Mtb strains that could be prospectively explored as biomarkers for TB protection or susceptibility.

Original languageEnglish
JournalEuropean Journal of Immunology
Volume44
Issue number5
Pages (from-to)1387-1398
Number of pages12
ISSN0014-2980
DOIs
Publication statusPublished - 2014

Keywords

  • Adolescent
  • Adult
  • Aged
  • Antigens, Bacterial
  • Bacterial Proteins
  • CD4-Positive T-Lymphocytes
  • Cytokines
  • Female
  • Humans
  • Male
  • Middle Aged
  • Mycobacterium tuberculosis
  • Species Specificity
  • Tuberculosis

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