Differential diagnosis of HIV type 1 group O and M infection by polymerase chain reaction and PstI restriction analysis of the pol gene fragments

L Heyndrickx; W Janssens; L Gürtler; L Zekeng; I Loussert-Ajaka; K Vereecken; B Willems; S Coppens; P Ndumbe; K Fransen; E Saman; M Alary; G van der Groen

doi:10.1089/aid.1998.14.973

Differential diagnosis of HIV type 1 group O and M infection by polymerase chain reaction and PstI restriction analysis of the pol gene fragments

L Heyndrickx, W Janssens, L Gürtler, L Zekeng, I Loussert-Ajaka, K Vereecken, B Willems, S Coppens, P Ndumbe, K Fransen, E Saman, M Alary, G van der Groen

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

HIV-1 group O serological screening or confirmation strategies so far have not proved 100% sensitive and specific, indicating a lack of antibody reactivity or cross-reactivity with group O antigens. Therefore, genetic analysis currently represents the only method by which confirm presumed HIV-1 group O or group O/M infections. We have optimized the sensitivity (100%) and specificity (100%) of an HIV-1 group O/M-specific PCR of a pol gene fragment. In addition, we report on a highly sensitive (97.2%) and specific (100%) method for differentiation between HIV-1 group O and group M viruses, using PCR and PstI enzyme restriction fragment analysis of a pol fragment. Compared with sequencing, these methods are fast, inexpensive, and simple.

Original language	English
Journal	AIDS Research and Human Retroviruses
Volume	14
Issue number	11
Pages (from-to)	973-977
ISSN	0889-2229
DOIs	https://doi.org/10.1089/aid.1998.14.973
Publication status	Published - 1998

Keywords

B780-tropical-medicine
Virology
HIV-1 group O
HIV-1 group M
Laboratory medicine
Diagnosis
PCR

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10.1089/aid.1998.14.973

http://lib.itg.be/pdf/itg/1998/1998arhr0973.pdf

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Heyndrickx, L., Janssens, W., Gürtler, L., Zekeng, L., Loussert-Ajaka, I., Vereecken, K., Willems, B., Coppens, S., Ndumbe, P., Fransen, K., Saman, E., Alary, M., & van der Groen, G. (1998). Differential diagnosis of HIV type 1 group O and M infection by polymerase chain reaction and PstI restriction analysis of the pol gene fragments. AIDS Research and Human Retroviruses, 14(11), 973-977. https://doi.org/10.1089/aid.1998.14.973

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title = "Differential diagnosis of HIV type 1 group O and M infection by polymerase chain reaction and PstI restriction analysis of the pol gene fragments",

abstract = "HIV-1 group O serological screening or confirmation strategies so far have not proved 100% sensitive and specific, indicating a lack of antibody reactivity or cross-reactivity with group O antigens. Therefore, genetic analysis currently represents the only method by which confirm presumed HIV-1 group O or group O/M infections. We have optimized the sensitivity (100%) and specificity (100%) of an HIV-1 group O/M-specific PCR of a pol gene fragment. In addition, we report on a highly sensitive (97.2%) and specific (100%) method for differentiation between HIV-1 group O and group M viruses, using PCR and PstI enzyme restriction fragment analysis of a pol fragment. Compared with sequencing, these methods are fast, inexpensive, and simple.",

keywords = "B780-tropical-medicine, Virology, HIV-1 group O, HIV-1 group M, Laboratory medicine, Diagnosis, PCR",

author = "L Heyndrickx and W Janssens and L G{\"u}rtler and L Zekeng and I Loussert-Ajaka and K Vereecken and B Willems and S Coppens and P Ndumbe and K Fransen and E Saman and M Alary and {van der Groen}, G",

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doi = "10.1089/aid.1998.14.973",

language = "English",

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pages = "973--977",

journal = "AIDS Research and Human Retroviruses",

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Heyndrickx, L, Janssens, W, Gürtler, L, Zekeng, L, Loussert-Ajaka, I, Vereecken, K, Willems, B, Coppens, S, Ndumbe, P, Fransen, K, Saman, E, Alary, M & van der Groen, G 1998, 'Differential diagnosis of HIV type 1 group O and M infection by polymerase chain reaction and PstI restriction analysis of the pol gene fragments', AIDS Research and Human Retroviruses, vol. 14, no. 11, pp. 973-977. https://doi.org/10.1089/aid.1998.14.973

TY - JOUR

T1 - Differential diagnosis of HIV type 1 group O and M infection by polymerase chain reaction and PstI restriction analysis of the pol gene fragments

AU - Heyndrickx, L

AU - Janssens, W

AU - Gürtler, L

AU - Zekeng, L

AU - Loussert-Ajaka, I

AU - Vereecken, K

AU - Willems, B

AU - Coppens, S

AU - Ndumbe, P

AU - Fransen, K

AU - Saman, E

AU - Alary, M

AU - van der Groen, G

PY - 1998

Y1 - 1998

N2 - HIV-1 group O serological screening or confirmation strategies so far have not proved 100% sensitive and specific, indicating a lack of antibody reactivity or cross-reactivity with group O antigens. Therefore, genetic analysis currently represents the only method by which confirm presumed HIV-1 group O or group O/M infections. We have optimized the sensitivity (100%) and specificity (100%) of an HIV-1 group O/M-specific PCR of a pol gene fragment. In addition, we report on a highly sensitive (97.2%) and specific (100%) method for differentiation between HIV-1 group O and group M viruses, using PCR and PstI enzyme restriction fragment analysis of a pol fragment. Compared with sequencing, these methods are fast, inexpensive, and simple.

AB - HIV-1 group O serological screening or confirmation strategies so far have not proved 100% sensitive and specific, indicating a lack of antibody reactivity or cross-reactivity with group O antigens. Therefore, genetic analysis currently represents the only method by which confirm presumed HIV-1 group O or group O/M infections. We have optimized the sensitivity (100%) and specificity (100%) of an HIV-1 group O/M-specific PCR of a pol gene fragment. In addition, we report on a highly sensitive (97.2%) and specific (100%) method for differentiation between HIV-1 group O and group M viruses, using PCR and PstI enzyme restriction fragment analysis of a pol fragment. Compared with sequencing, these methods are fast, inexpensive, and simple.

KW - B780-tropical-medicine

KW - Virology

KW - HIV-1 group O

KW - HIV-1 group M

KW - Laboratory medicine

KW - Diagnosis

KW - PCR

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000074874300007

U2 - 10.1089/aid.1998.14.973

DO - 10.1089/aid.1998.14.973

M3 - A1: Web of Science-article

SN - 0889-2229

VL - 14

SP - 973

EP - 977

JO - AIDS Research and Human Retroviruses

JF - AIDS Research and Human Retroviruses

IS - 11

ER -

Differential diagnosis of HIV type 1 group O and M infection by polymerase chain reaction and PstI restriction analysis of the pol gene fragments

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