Distribution of serotypes and antibiotic resistance of invasive Pseudomonas aeruginosa in a multi-country collection

Shamima Nasrin; Nicolas Hegerle; Shaichi Sen; Joseph Nkeze; Sunil Sen; Jasnehta Permala-Booth; Myeongjin Choi; James Sinclair; Milagritos D Tapia; J Kristie Johnson; Samba O Sow; Joshua T Thaden; Vance G Fowler; Karen A Krogfelt; Annelie Brauner; Efthymia Protonotariou; Eirini Christaki; Yuichiro Shindo; Andrea L Kwa; Sadia Shakoor; Ashika Singh-Moodley; Olga Perovic; Jan Jacobs; Octavie Lunguya; Raphael Simon; Alan S Cross; Sharon M Tennant

doi:10.1186/s12866-021-02427-4

Distribution of serotypes and antibiotic resistance of invasive Pseudomonas aeruginosa in a multi-country collection

Shamima Nasrin, Nicolas Hegerle, Shaichi Sen, Joseph Nkeze, Sunil Sen, Jasnehta Permala-Booth, Myeongjin Choi, James Sinclair, Milagritos D Tapia, J Kristie Johnson, Samba O Sow, Joshua T Thaden, Vance G Fowler, Karen A Krogfelt, Annelie Brauner, Efthymia Protonotariou, Eirini Christaki, Yuichiro Shindo, Andrea L Kwa, Sadia ShakoorAshika Singh-Moodley, Olga Perovic, Jan Jacobs, Octavie Lunguya, Raphael Simon, Alan S Cross, Sharon M Tennant

Tropical Bacteriology

Research output: Contribution to journal › A1: Web of Science-article › peer-review

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Abstract

BACKGROUND: Pseudomonas aeruginosa is an opportunistic pathogen that causes a wide range of acute and chronic infections and is frequently associated with healthcare-associated infections. Because of its ability to rapidly acquire resistance to antibiotics, P. aeruginosa infections are difficult to treat. Alternative strategies, such as a vaccine, are needed to prevent infections. We collected a total of 413 P. aeruginosa isolates from the blood and cerebrospinal fluid of patients from 10 countries located on 4 continents during 2005-2017 and characterized these isolates to inform vaccine development efforts. We determined the diversity and distribution of O antigen and flagellin types and antibiotic susceptibility of the invasive P. aeruginosa. We used an antibody-based agglutination assay and PCR for O antigen typing and PCR for flagellin typing. We determined antibiotic susceptibility using the Kirby-Bauer disk diffusion method.

RESULTS: Of the 413 isolates, 314 (95%) were typed by an antibody-based agglutination assay or PCR (n = 99). Among the 20 serotypes of P. aeruginosa, the most common serotypes were O1, O2, O3, O4, O5, O6, O8, O9, O10 and O11; a vaccine that targets these 10 serotypes would confer protection against more than 80% of invasive P. aeruginosa infections. The most common flagellin type among 386 isolates was FlaB (41%). Resistance to aztreonam (56%) was most common, followed by levofloxacin (42%). We also found that 22% of strains were non-susceptible to meropenem and piperacillin-tazobactam. Ninety-nine (27%) of our collected isolates were resistant to multiple antibiotics. Isolates with FlaA2 flagellin were more commonly multidrug resistant (p = 0.04).

CONCLUSIONS: Vaccines targeting common O antigens and two flagellin antigens, FlaB and FlaA2, would offer an excellent strategy to prevent P. aeruginosa invasive infections.

Original language	English
Article number	13
Journal	BMC Microbiology
Volume	22
Issue number	1
Pages (from-to)	1-12
Number of pages	12
ISSN	1471-2180
DOIs	https://doi.org/10.1186/s12866-021-02427-4
Publication status	Published - 2022

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2022bmic0013Final published version, 1.45 MBLicence: CC BY

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Nasrin, S., Hegerle, N., Sen, S., Nkeze, J., Sen, S., Permala-Booth, J., Choi, M., Sinclair, J., Tapia, M. D., Johnson, J. K., Sow, S. O., Thaden, J. T., Fowler, V. G., Krogfelt, K. A., Brauner, A., Protonotariou, E., Christaki, E., Shindo, Y., Kwa, A. L., ... Tennant, S. M. (2022). Distribution of serotypes and antibiotic resistance of invasive Pseudomonas aeruginosa in a multi-country collection. BMC Microbiology, 22(1), 1-12. Article 13. https://doi.org/10.1186/s12866-021-02427-4

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title = "Distribution of serotypes and antibiotic resistance of invasive Pseudomonas aeruginosa in a multi-country collection",

abstract = "BACKGROUND: Pseudomonas aeruginosa is an opportunistic pathogen that causes a wide range of acute and chronic infections and is frequently associated with healthcare-associated infections. Because of its ability to rapidly acquire resistance to antibiotics, P. aeruginosa infections are difficult to treat. Alternative strategies, such as a vaccine, are needed to prevent infections. We collected a total of 413 P. aeruginosa isolates from the blood and cerebrospinal fluid of patients from 10 countries located on 4 continents during 2005-2017 and characterized these isolates to inform vaccine development efforts. We determined the diversity and distribution of O antigen and flagellin types and antibiotic susceptibility of the invasive P. aeruginosa. We used an antibody-based agglutination assay and PCR for O antigen typing and PCR for flagellin typing. We determined antibiotic susceptibility using the Kirby-Bauer disk diffusion method.RESULTS: Of the 413 isolates, 314 (95%) were typed by an antibody-based agglutination assay or PCR (n = 99). Among the 20 serotypes of P. aeruginosa, the most common serotypes were O1, O2, O3, O4, O5, O6, O8, O9, O10 and O11; a vaccine that targets these 10 serotypes would confer protection against more than 80% of invasive P. aeruginosa infections. The most common flagellin type among 386 isolates was FlaB (41%). Resistance to aztreonam (56%) was most common, followed by levofloxacin (42%). We also found that 22% of strains were non-susceptible to meropenem and piperacillin-tazobactam. Ninety-nine (27%) of our collected isolates were resistant to multiple antibiotics. Isolates with FlaA2 flagellin were more commonly multidrug resistant (p = 0.04).CONCLUSIONS: Vaccines targeting common O antigens and two flagellin antigens, FlaB and FlaA2, would offer an excellent strategy to prevent P. aeruginosa invasive infections.",

author = "Shamima Nasrin and Nicolas Hegerle and Shaichi Sen and Joseph Nkeze and Sunil Sen and Jasnehta Permala-Booth and Myeongjin Choi and James Sinclair and Tapia, {Milagritos D} and Johnson, {J Kristie} and Sow, {Samba O} and Thaden, {Joshua T} and Fowler, {Vance G} and Krogfelt, {Karen A} and Annelie Brauner and Efthymia Protonotariou and Eirini Christaki and Yuichiro Shindo and Kwa, {Andrea L} and Sadia Shakoor and Ashika Singh-Moodley and Olga Perovic and Jan Jacobs and Octavie Lunguya and Raphael Simon and Cross, {Alan S} and Tennant, {Sharon M}",

note = "FTX; DOAJ; (CC BY 4.0); {\textcopyright} 2022. The Author(s).",

year = "2022",

doi = "10.1186/s12866-021-02427-4",

language = "English",

volume = "22",

pages = "1--12",

journal = "BMC Microbiology",

issn = "1471-2180",

publisher = "BioMed Central",

number = "1",

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Nasrin, S, Hegerle, N, Sen, S, Nkeze, J, Sen, S, Permala-Booth, J, Choi, M, Sinclair, J, Tapia, MD, Johnson, JK, Sow, SO, Thaden, JT, Fowler, VG, Krogfelt, KA, Brauner, A, Protonotariou, E, Christaki, E, Shindo, Y, Kwa, AL, Shakoor, S, Singh-Moodley, A, Perovic, O, Jacobs, J, Lunguya, O, Simon, R, Cross, AS & Tennant, SM 2022, 'Distribution of serotypes and antibiotic resistance of invasive Pseudomonas aeruginosa in a multi-country collection', BMC Microbiology, vol. 22, no. 1, 13, pp. 1-12. https://doi.org/10.1186/s12866-021-02427-4

TY - JOUR

T1 - Distribution of serotypes and antibiotic resistance of invasive Pseudomonas aeruginosa in a multi-country collection

AU - Nasrin, Shamima

AU - Hegerle, Nicolas

AU - Sen, Shaichi

AU - Nkeze, Joseph

AU - Sen, Sunil

AU - Permala-Booth, Jasnehta

AU - Choi, Myeongjin

AU - Sinclair, James

AU - Tapia, Milagritos D

AU - Johnson, J Kristie

AU - Sow, Samba O

AU - Thaden, Joshua T

AU - Fowler, Vance G

AU - Krogfelt, Karen A

AU - Brauner, Annelie

AU - Protonotariou, Efthymia

AU - Christaki, Eirini

AU - Shindo, Yuichiro

AU - Kwa, Andrea L

AU - Shakoor, Sadia

AU - Singh-Moodley, Ashika

AU - Perovic, Olga

AU - Jacobs, Jan

AU - Lunguya, Octavie

AU - Simon, Raphael

AU - Cross, Alan S

AU - Tennant, Sharon M

PY - 2022

Y1 - 2022

N2 - BACKGROUND: Pseudomonas aeruginosa is an opportunistic pathogen that causes a wide range of acute and chronic infections and is frequently associated with healthcare-associated infections. Because of its ability to rapidly acquire resistance to antibiotics, P. aeruginosa infections are difficult to treat. Alternative strategies, such as a vaccine, are needed to prevent infections. We collected a total of 413 P. aeruginosa isolates from the blood and cerebrospinal fluid of patients from 10 countries located on 4 continents during 2005-2017 and characterized these isolates to inform vaccine development efforts. We determined the diversity and distribution of O antigen and flagellin types and antibiotic susceptibility of the invasive P. aeruginosa. We used an antibody-based agglutination assay and PCR for O antigen typing and PCR for flagellin typing. We determined antibiotic susceptibility using the Kirby-Bauer disk diffusion method.RESULTS: Of the 413 isolates, 314 (95%) were typed by an antibody-based agglutination assay or PCR (n = 99). Among the 20 serotypes of P. aeruginosa, the most common serotypes were O1, O2, O3, O4, O5, O6, O8, O9, O10 and O11; a vaccine that targets these 10 serotypes would confer protection against more than 80% of invasive P. aeruginosa infections. The most common flagellin type among 386 isolates was FlaB (41%). Resistance to aztreonam (56%) was most common, followed by levofloxacin (42%). We also found that 22% of strains were non-susceptible to meropenem and piperacillin-tazobactam. Ninety-nine (27%) of our collected isolates were resistant to multiple antibiotics. Isolates with FlaA2 flagellin were more commonly multidrug resistant (p = 0.04).CONCLUSIONS: Vaccines targeting common O antigens and two flagellin antigens, FlaB and FlaA2, would offer an excellent strategy to prevent P. aeruginosa invasive infections.

AB - BACKGROUND: Pseudomonas aeruginosa is an opportunistic pathogen that causes a wide range of acute and chronic infections and is frequently associated with healthcare-associated infections. Because of its ability to rapidly acquire resistance to antibiotics, P. aeruginosa infections are difficult to treat. Alternative strategies, such as a vaccine, are needed to prevent infections. We collected a total of 413 P. aeruginosa isolates from the blood and cerebrospinal fluid of patients from 10 countries located on 4 continents during 2005-2017 and characterized these isolates to inform vaccine development efforts. We determined the diversity and distribution of O antigen and flagellin types and antibiotic susceptibility of the invasive P. aeruginosa. We used an antibody-based agglutination assay and PCR for O antigen typing and PCR for flagellin typing. We determined antibiotic susceptibility using the Kirby-Bauer disk diffusion method.RESULTS: Of the 413 isolates, 314 (95%) were typed by an antibody-based agglutination assay or PCR (n = 99). Among the 20 serotypes of P. aeruginosa, the most common serotypes were O1, O2, O3, O4, O5, O6, O8, O9, O10 and O11; a vaccine that targets these 10 serotypes would confer protection against more than 80% of invasive P. aeruginosa infections. The most common flagellin type among 386 isolates was FlaB (41%). Resistance to aztreonam (56%) was most common, followed by levofloxacin (42%). We also found that 22% of strains were non-susceptible to meropenem and piperacillin-tazobactam. Ninety-nine (27%) of our collected isolates were resistant to multiple antibiotics. Isolates with FlaA2 flagellin were more commonly multidrug resistant (p = 0.04).CONCLUSIONS: Vaccines targeting common O antigens and two flagellin antigens, FlaB and FlaA2, would offer an excellent strategy to prevent P. aeruginosa invasive infections.

U2 - 10.1186/s12866-021-02427-4

DO - 10.1186/s12866-021-02427-4

M3 - A1: Web of Science-article

C2 - 34991476

SN - 1471-2180

VL - 22

SP - 1

EP - 12

JO - BMC Microbiology

JF - BMC Microbiology

IS - 1

M1 - 13

ER -

Distribution of serotypes and antibiotic resistance of invasive Pseudomonas aeruginosa in a multi-country collection

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