Early diagnosis and follow-up of acute schistosomiasis in a cluster of infected Belgian travellers by detection of antibodies and Circulating Anodic Antigen (CAA): a diagnostic evaluation study

Pytsje T Hoekstra; Marjan van Esbroeck; Claudia J de Dood; Paul Lam Corstjens; Lieselotte Cnops; Christel Jg van Zeijl-van der Ham; Linda J Wammes; Govert J van Dam; Jan Clerinx; Lisette van Lieshout

doi:10.1016/j.tmaid.2021.102053

Early diagnosis and follow-up of acute schistosomiasis in a cluster of infected Belgian travellers by detection of antibodies and Circulating Anodic Antigen (CAA): a diagnostic evaluation study

Pytsje T Hoekstra
, Marjan van Esbroeck
, Claudia J de Dood
, Paul Lam Corstjens
, Lieselotte Cnops
, Christel Jg van Zeijl-van der Ham
, Linda J Wammes
, Govert J van Dam
, Jan Clerinx
, Lisette van Lieshout

Research output: Contribution to journal › A1: Peer-reviewed journal articles › peer-review

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Abstract

BACKGROUND: In order to evaluate the diagnostic value of schistosome circulating anodic antigen (CAA) detection, serum and urine CAA levels were determined in a single cluster of 34 Belgian tourists at three timepoints within a period of 14 weeks following proven Schistosoma exposure in South Africa and compared with two in-house antibody assays.

METHODS: Samples were collected 4-5 and 7-8 weeks post-exposure and subsequently 5-6 weeks following praziquantel treatment. Schistosoma antibodies were detected by an adult worm antigen-immunofluorescence assay (AWA-IFA) and a soluble egg antigen-enzyme-linked immunosorbent assay (SEA-ELISA), while CAA concentrations were determined by the Up-Converting reporter Particle labelled Lateral Flow (UCP-LF) test.

RESULTS: Antibodies were detected in 25/34 (73%) travellers pre-treatment and in 27/34 (79%) post-treatment, with the AWA-IFA showing better performance than the SEA-ELISA. Pre-treatment, CAA was detected in 13/34 (38%) and 33/34 (97%) of the travellers in urine and serum, respectively. Post-treatment, all except one traveller became serum CAA negative. This in contrast to the detected antibodies, as well as the previously reported diagnostic results of this cluster.

CONCLUSIONS: The UCP-LF CAA serum assay has been demonstrated as the most sensitive method for the diagnosis of early Schistosoma infections and post-treatment monitoring in travellers.

Original language	English
Article number	102053
Journal	Travel Medicine and Infectious Disease
Volume	41
Number of pages	6
ISSN	1477-8939
DOIs	https://doi.org/10.1016/j.tmaid.2021.102053
Publication status	Published - 2021

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2021tmid102053Final published version, 1.49 MBLicence: CC BY

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Hoekstra, P. T., van Esbroeck, M., de Dood, C. J., Corstjens, P. L., Cnops, L., van Zeijl-van der Ham, C. J., Wammes, L. J., van Dam, G. J., Clerinx, J., & van Lieshout, L. (2021). Early diagnosis and follow-up of acute schistosomiasis in a cluster of infected Belgian travellers by detection of antibodies and Circulating Anodic Antigen (CAA): a diagnostic evaluation study. Travel Medicine and Infectious Disease, 41, Article 102053. https://doi.org/10.1016/j.tmaid.2021.102053

@article{7ce83c5981ae4cf4854f6fa890a3a98f,

title = "Early diagnosis and follow-up of acute schistosomiasis in a cluster of infected Belgian travellers by detection of antibodies and Circulating Anodic Antigen (CAA): a diagnostic evaluation study",

abstract = "BACKGROUND: In order to evaluate the diagnostic value of schistosome circulating anodic antigen (CAA) detection, serum and urine CAA levels were determined in a single cluster of 34 Belgian tourists at three timepoints within a period of 14 weeks following proven Schistosoma exposure in South Africa and compared with two in-house antibody assays.METHODS: Samples were collected 4-5 and 7-8 weeks post-exposure and subsequently 5-6 weeks following praziquantel treatment. Schistosoma antibodies were detected by an adult worm antigen-immunofluorescence assay (AWA-IFA) and a soluble egg antigen-enzyme-linked immunosorbent assay (SEA-ELISA), while CAA concentrations were determined by the Up-Converting reporter Particle labelled Lateral Flow (UCP-LF) test.RESULTS: Antibodies were detected in 25/34 (73\%) travellers pre-treatment and in 27/34 (79\%) post-treatment, with the AWA-IFA showing better performance than the SEA-ELISA. Pre-treatment, CAA was detected in 13/34 (38\%) and 33/34 (97\%) of the travellers in urine and serum, respectively. Post-treatment, all except one traveller became serum CAA negative. This in contrast to the detected antibodies, as well as the previously reported diagnostic results of this cluster.CONCLUSIONS: The UCP-LF CAA serum assay has been demonstrated as the most sensitive method for the diagnosis of early Schistosoma infections and post-treatment monitoring in travellers.",

author = "Hoekstra, \{Pytsje T\} and \{van Esbroeck\}, Marjan and \{de Dood\}, \{Claudia J\} and Corstjens, \{Paul Lam\} and Lieselotte Cnops and \{van Zeijl-van der Ham\}, \{Christel Jg\} and Wammes, \{Linda J\} and \{van Dam\}, \{Govert J\} and Jan Clerinx and \{van Lieshout\}, Lisette",

year = "2021",

doi = "10.1016/j.tmaid.2021.102053",

language = "English",

volume = "41",

journal = "Travel Medicine and Infectious Disease",

issn = "1477-8939",

publisher = "Elsevier Inc.",

}

Hoekstra, PT, van Esbroeck, M, de Dood, CJ, Corstjens, PL, Cnops, L, van Zeijl-van der Ham, CJ, Wammes, LJ, van Dam, GJ, Clerinx, J & van Lieshout, L 2021, 'Early diagnosis and follow-up of acute schistosomiasis in a cluster of infected Belgian travellers by detection of antibodies and Circulating Anodic Antigen (CAA): a diagnostic evaluation study', Travel Medicine and Infectious Disease, vol. 41, 102053. https://doi.org/10.1016/j.tmaid.2021.102053

Early diagnosis and follow-up of acute schistosomiasis in a cluster of infected Belgian travellers by detection of antibodies and Circulating Anodic Antigen (CAA): a diagnostic evaluation study. / Hoekstra, Pytsje T; van Esbroeck, Marjan; de Dood, Claudia J et al.
In: Travel Medicine and Infectious Disease, Vol. 41, 102053, 2021.

Research output: Contribution to journal › A1: Peer-reviewed journal articles › peer-review

TY - JOUR

T1 - Early diagnosis and follow-up of acute schistosomiasis in a cluster of infected Belgian travellers by detection of antibodies and Circulating Anodic Antigen (CAA): a diagnostic evaluation study

AU - Hoekstra, Pytsje T

AU - van Esbroeck, Marjan

AU - de Dood, Claudia J

AU - Corstjens, Paul Lam

AU - Cnops, Lieselotte

AU - van Zeijl-van der Ham, Christel Jg

AU - Wammes, Linda J

AU - van Dam, Govert J

AU - Clerinx, Jan

AU - van Lieshout, Lisette

PY - 2021

Y1 - 2021

N2 - BACKGROUND: In order to evaluate the diagnostic value of schistosome circulating anodic antigen (CAA) detection, serum and urine CAA levels were determined in a single cluster of 34 Belgian tourists at three timepoints within a period of 14 weeks following proven Schistosoma exposure in South Africa and compared with two in-house antibody assays.METHODS: Samples were collected 4-5 and 7-8 weeks post-exposure and subsequently 5-6 weeks following praziquantel treatment. Schistosoma antibodies were detected by an adult worm antigen-immunofluorescence assay (AWA-IFA) and a soluble egg antigen-enzyme-linked immunosorbent assay (SEA-ELISA), while CAA concentrations were determined by the Up-Converting reporter Particle labelled Lateral Flow (UCP-LF) test.RESULTS: Antibodies were detected in 25/34 (73%) travellers pre-treatment and in 27/34 (79%) post-treatment, with the AWA-IFA showing better performance than the SEA-ELISA. Pre-treatment, CAA was detected in 13/34 (38%) and 33/34 (97%) of the travellers in urine and serum, respectively. Post-treatment, all except one traveller became serum CAA negative. This in contrast to the detected antibodies, as well as the previously reported diagnostic results of this cluster.CONCLUSIONS: The UCP-LF CAA serum assay has been demonstrated as the most sensitive method for the diagnosis of early Schistosoma infections and post-treatment monitoring in travellers.

AB - BACKGROUND: In order to evaluate the diagnostic value of schistosome circulating anodic antigen (CAA) detection, serum and urine CAA levels were determined in a single cluster of 34 Belgian tourists at three timepoints within a period of 14 weeks following proven Schistosoma exposure in South Africa and compared with two in-house antibody assays.METHODS: Samples were collected 4-5 and 7-8 weeks post-exposure and subsequently 5-6 weeks following praziquantel treatment. Schistosoma antibodies were detected by an adult worm antigen-immunofluorescence assay (AWA-IFA) and a soluble egg antigen-enzyme-linked immunosorbent assay (SEA-ELISA), while CAA concentrations were determined by the Up-Converting reporter Particle labelled Lateral Flow (UCP-LF) test.RESULTS: Antibodies were detected in 25/34 (73%) travellers pre-treatment and in 27/34 (79%) post-treatment, with the AWA-IFA showing better performance than the SEA-ELISA. Pre-treatment, CAA was detected in 13/34 (38%) and 33/34 (97%) of the travellers in urine and serum, respectively. Post-treatment, all except one traveller became serum CAA negative. This in contrast to the detected antibodies, as well as the previously reported diagnostic results of this cluster.CONCLUSIONS: The UCP-LF CAA serum assay has been demonstrated as the most sensitive method for the diagnosis of early Schistosoma infections and post-treatment monitoring in travellers.

U2 - 10.1016/j.tmaid.2021.102053

DO - 10.1016/j.tmaid.2021.102053

M3 - A1: Peer-reviewed journal articles

C2 - 33823289

SN - 1477-8939

VL - 41

JO - Travel Medicine and Infectious Disease

JF - Travel Medicine and Infectious Disease

M1 - 102053

ER -

Hoekstra PT, van Esbroeck M, de Dood CJ, Corstjens PL, Cnops L, van Zeijl-van der Ham CJ et al. Early diagnosis and follow-up of acute schistosomiasis in a cluster of infected Belgian travellers by detection of antibodies and Circulating Anodic Antigen (CAA): a diagnostic evaluation study. Travel Medicine and Infectious Disease. 2021;41:102053. doi: 10.1016/j.tmaid.2021.102053

Early diagnosis and follow-up of acute schistosomiasis in a cluster of infected Belgian travellers by detection of antibodies and Circulating Anodic Antigen (CAA): a diagnostic evaluation study

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